Tissue/cell: Rat eye tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-NRL Polyclonal Antibody, Unconjugated(bs-19351R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-19351R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-19351R |
| 英文名称 | Neural retinal specific leucine zipper/NRL Rabbit pAb |
| 中文名称 | 神经视网膜特定亮氨酸拉链蛋白抗体 |
| 英文别名 | D14S46E; Neural retina-specific leucine zipper protein; Neural retinal specific leucine zipper; NRL; NRL MAF; NRL_HUMAN; RP27. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Neural retinal specific leucine zipper/NRL |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 26 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > Leucine Zipper Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Neuroscience > Sensory System > Visual system |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Neural retina. |
| 相似性 | Belongs to the bZIP family.
Contains 1 bZIP domain. |
| 功能 | Transcription factor which regulates the expression of several rod-specific genes, in cluding RHO and PDE6B. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a basic motif-leucine zipper transcription factor of the Maf subfamily. The encoded protein is conserved among vertebrates and is a critical intrinsic regulator of photoceptor development and function. Mutations in this gene have been associated with retinitis pigmentosa and retinal degenerative diseases. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample: Raji (human)cell Lysate at 40 ug
Primary: Anti-NRL(bs-19351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 30 kD
Primary: Anti-NRL(bs-19351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 30 kD
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
secondary antibody review -- data from 99 publications
data
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
. Berton, T.R., Matsumoto, T., Page, A., Conti, C.J., Deng, C.X., Jorcano, J.L., and Johnson, D.G. 2003. Tumor formation in mice with conditional inactivation of Brca1 in epithelial tissues. Oncogene 22:5415
技术资料暂无技术资料 索取技术资料
Neural retinal specific leucine zipper/NRL Rabbit pAb(bs-19351R)-50ul/100ul/200ul
￥1180 - 2800
