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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-18638R |
| 英文名称 | MAK3/C14orf35 Rabbit pAb |
| 中文名称 | 14号染色体开放阅框35抗体 |
| 英文别名 | C14orf35; Chromosome 14 open reading frame 35; FLJ35355; MAK 3; Mak3p; MGC141884; MGC141885; N acetyltransferase 12(GCN5 related putative); N acetyltransferase 12; N acetyltransferase MAK3 homolog; NAT12; Putative N acetyltransferase. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MAK3/C14orf35 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 39 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of NAA35, NAA38 and NAA30. |
| 亚细胞定位 | Cytoplasmic |
| 相似性 | Belongs to the acetyltransferase family. MAK3 subfamily. _x000D_ Contains 1 N-acetyltransferase domain._x000D_ |
| 功能 | Catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. Catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. Necessary for the lysosomal localization and function of ARL8B sugeesting that ARL8B is a NatC substrate. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | MAK3 belongs to the acetyltransferase family, MAK3 subfamily. It contains one N-acetyltransferase domain. It is a probable N-acetyltransferase. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验expression by a CREB-induced microRNA". Nature neuroscience 10 (12): 1513–1514. doi:10.1038/nn2010. ISSN 1097-6256. PMID 17994015. edit ^ You Y, Moreira BG, Behlke MA, Owczarzy R (2006). "Design of LNA probes that improve mismatch discrimination". Nucleic
of H2O. Add 5 units of Taq DNA polymerase. Amplify for 30-35 cycles in a thermal cycler. Take 5 µl of PCR product and add 2 µl of 6X DNA loading buffer. Adjust the volume to 12 µl with H2O. Load the samples onto a 1% (w/v) agarose gel (~10-15 cm
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. Harvest ESCs as a single cell suspension by trypsin-mediated disaggregation (Step 22). Resuspend the cells to a concentration of 5 x 104 cells per mL of OP9 medium. 30. Seed 1 mL of ESCs per 10-cm dish of OP9 cells, and return
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