_x000D_
Sample:
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Heart (Mouse) Lysate at 40 ug
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Primary: Anti-LMAN1L (bs-18305R) at 1/300 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 55 kD
_x000D_
Observed band size: 55 kD
- ￥1180 - 2800
- Bioss
- bs-18305R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-18305R |
| 英文名称 | LMAN1L Rabbit pAb |
| 中文名称 | 凝集素甘露糖结合蛋白1样蛋白抗体 |
| 英文别名 | LMAN1L; ERGIC-53-like protein; ERGIC-53L; ERGIC53-like protein; ERGL; lectin, mannose-binding, 1 like; LMAN1-like protein; protein ERGIC-53-like; UNQ2784/PRO7174. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LMAN1L |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LMAN1L) Polyclonal Antibody, Unconjugated (bs-18305R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验相关实验
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
. Berton, T.R., Matsumoto, T., Page, A., Conti, C.J., Deng, C.X., Jorcano, J.L., and Johnson, D.G. 2003. Tumor formation in mice with conditional inactivation of Brca1 in epithelial tissues. Oncogene 22:5415
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LMAN1L | ERGIC-53L Rabbit pAb(bs-18305R)-50ul/100ul/200ul
￥1180 - 2800
