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- 技术资料
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-17836R |
| 英文名称 | MRS2 Rabbit pAb |
| 中文名称 | MRS2蛋白抗体 |
| 英文别名 | Gm902; HPT; Magnesium transporter MRS2 homolog, mitochondrial; MGC78523; MRS2 like protein; MRS2 like, magnesium homeostasis factor; MRS2 like, magnesium homeostasis factor(S. cerevisiae); MRS2 magnesium homeostasis factor homolog(S. cerevisiae); MRS2L; RPT |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Pig, Cow, Horse, Rabbit, Sheep, Cat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MRS2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Vitamins / minerals Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Signal Transduction > Metabolism > Mitochondrial Signal Transduction > Metabolism > Vitamins / Minerals |
| 亚细胞定位 | Mitochondrion inner membrane; Multi-pass membrane protein (by similarity). |
| 相似性 | Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family. _x000D_ |
| 功能 | Magnesium transporter that may mediate the influx of magnesium into the mitochondrial matrix._x000D_ |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | MRS2 is a magnesium transporter that may mediate the influx of magnesium into the mitochondrial matrix. There are 3 isoforms produced by alternative splicing. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRS2 ) Polyclonal Antibody, Unconjugated (bs-17836R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining._x000D_
_x000D_

Sample: Pancreas (mouse) Lysate at 40 ug
_x000D_ Primary: Anti- MRS2(bs-17836R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50kD
_x000D_ Observed band size: 50 kD
_x000D_
_x000D_ Primary: Anti- MRS2(bs-17836R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50kD
_x000D_ Observed band size: 50 kD
_x000D_

Sample:
_x000D_ Lane 1: Mouse Muscle tissue lysates
_x000D_ Lane 2: Mouse Heart tissue lysates
_x000D_ Lane 3: Rat Muscle tissue lysates
_x000D_ Lane 4: Rat Heart tissue lysates
_x000D_ Primary: Anti-MRS2 (bs-17836R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kDa
_x000D_ Observed band size: 50 kDa
_x000D_ Lane 1: Mouse Muscle tissue lysates
_x000D_ Lane 2: Mouse Heart tissue lysates
_x000D_ Lane 3: Rat Muscle tissue lysates
_x000D_ Lane 4: Rat Heart tissue lysates
_x000D_ Primary: Anti-MRS2 (bs-17836R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kDa
_x000D_ Observed band size: 50 kDa
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MRS2 | MRS2L Rabbit pAb(bs-17836R)-50ul/100ul/200ul
¥1180 - 2800






