Sample: Ovary (Mouse)Lysate at 40 ug
Primary: Anti-Zonapellucida glycoprotein 4(bs-4470R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
- ￥1180 - 2800
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- bs-4470R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4470R |
| 英文名称 | Zona pellucida glycoprotein 4 Rabbit pAb |
| 中文名称 | 卵透明带糖蛋白4抗体 |
| 英文别名 | Processed zona pellucida sperm-binding protein 4; ZBP; Zona pellucida B protein; Zona pellucida glycoprotein 4; Zona pellucida protein B; Zona pellucida sperm binding protein 4; Zp-4; ZP1; ZP4; ZP4_HUMAN; ZPB. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ZP4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 57 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Secreted > extracellular space > extracellular matrix and Cell membrane. |
| 组织特异性 | Oocytes. |
| 翻译后修饰 | Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. |
| 相似性 | Belongs to the ZP domain family. ZPB subfamily. Contains 1 P-type (trefoil) domain. Contains 1 ZP domain. |
| 功能 | The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample: Uterus(Mouse)Lysate at 40 ug
Primary: Anti-Zonapellucida glycoprotein 4(bs-4470R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
Primary: Anti-Zonapellucida glycoprotein 4(bs-4470R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
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文献和实验相关实验
【申请上传】Current Protocols in Immunology
Autoimmunity Unit 15.16 The Day-3 Thymectomy Model for Induction of Multiple Organ-Specific Autoimmune Diseases Unit 15.17 Autoimmune Ovarian Disease Induced by Immunization with Zona Pellucida (ZP3 Peptide Unit 15.18 Animal Models of Airway Sensitization
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Zona pellucida glycoprotein 4 Rabbit pAb(bs-4470R)-50ul/100ul/200ul
￥1180 - 2800
