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phospho-eIF2B epsilon (Ser539)

Rabbit pAb(bs-14519R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-14519R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-14519R
    英文名称phospho-eIF2B epsilon (Ser539) Rabbit pAb
    中文名称磷酸化eIF2B蛋白抗体
    英文别名eIF2B epsilon(phospho S539); CACH; CLE; EI2BE_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor; eIF 2B GDP GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2 beta Epsilon; Eukaryotic Translation Initiation Factor 2 beta Epsilon; Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa; Eukaryotic translation initiation factor 2B subunit 5 epsilon; LVWM; Translation initiation factor eIF 2B subunit epsilon; Translation initiation factor eIF-2B subunit epsilon.
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human EFHC1 around the phosphorylation site of Ser539
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量80 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation

    Epigenetics and Nuclear Signaling > RNAi > Eukaryotic Initiation factors (eIF's)

    亚基Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2.
    组织特异性Widely expressed. Not detected in lymphocytes.
    翻译后修饰Phosphorylated at Ser-544 by DYRK2; this is required for subsequent phosphorylation by GSK3B (By similarity). Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function.
    Polyubiquitinated, probably by NEDD4 (By similarity).
    相似性Belongs to the eIF-2B gamma/epsilon subunits family.
    Contains 1 W2 domain.
    功能Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009]

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-eIF2B epsilon (Ser539)) Polyclonal Antibody, Unconjugated (bs-14519R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-eIF2B epsilon (Ser539)) Polyclonal Antibody, Unconjugated (bs-14519R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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    该产品被引用文献

    [IF={{ 15.304 }}] {Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635} {WB} {Mouse}

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    文献支持
    phospho-eIF2B epsilon (Ser539) Rabbit pAb(bs-14519R)-50ul/100ul/200ul
    ￥1180 - 2800