Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NROB2 Polyclonal Antibody, Unconjugated(bs-4311R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-4311R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4311R |
| 英文名称 | NR0B2 Rabbit pAb |
| 中文名称 | 核受体0相关蛋白B家族2抗体 |
| 英文别名 | NR0B2; NR0B2_HUMAN; Nr0b2a; Nuclear receptor subfamily 0 group B member 2; Nuclear receptor subfamily 0, group B, member 2a; Orphan nuclear receptor SHP; SHP; SHP-1; Shp1; Small heterodimer partner. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NR0B2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 28 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Retinoic & Retinoid Signal Transduction > Metabolism > Lipid metabolism Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Retinoic & Retinoid |
| 亚基 | Interacts with RARA, RXRA, THRB, NR5A1, NR5A2, NR1I3, PPARA, PPARG and EID1. May also interact with HNF4A (By similarity). Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. |
| 亚细胞定位 | Nucleus. Cytoplasm. Note=Colocalizes with NEUROD1 in the nucleus. |
| 组织特异性 | Liver. Low levels of expression were detected in heart and pancreas. |
| 相似性 | Belongs to the nuclear hormone receptor family. NR0 subfamily. |
| 功能 | Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SHP is an orphan nuclear receptor containing the dimerization and ligand-binding domains found in other nuclear receptors, but lacking the conserved DNA binding domain. SHP is specifically expressed in liver and other tissues, including fetal liver and adrenal gland, as well as adult spleen and small intestine. In addition, SHP is highy expressed in the murine macrophage cell line RAW 264.7 but suppressed by oxLDL and 13-HODE, which is a ligand for PPARg. SHP interacts with nuclear receptors, including thyroid receptor, retinoic acid receptors (RAR and RXR) and estrogen receptors (ERa and ERb). SHP functions as a negative regulator of these receptors by at least three mechanisms: inhibition of DNA binding via dimerization, direct antagonism of coactivator function through competition and possibly transrepression via recruitment of putative corepressors. In oxLDL-treated, resting macrophage cells, SHP acts as a transcription coactivator of NFkB, suggesting that SHP is a modulatory component in the regulation of the transcriptional activities of NFkB. Lastly, negative feedback regulation of a hepatic bile acid transporter, NTCP, is controlled by bile acid-activated FXR via induction of SHP to protect the hepatocyte from bile acid-mediated damage in cholestatic conditions. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR0B2) Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR0B2) Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Small intestine tissue lysates
Primary: Anti-NR0B2 (bs-4311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Small intestine tissue lysates
Primary: Anti-NR0B2 (bs-4311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
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文献和实验该产品被引用文献
[IF={{ 6.876 }}] {Song Guochao. et al. Potential therapeutic action of tauroursodeoxycholic acid against cholestatic liver injury via hepatic Fxr/Nrf2 and CHOP-DR5-caspase-8 pathway. CLIN SCI. 2023 Apr;137(7):561-577} {WB} {Mouse}
[IF={{ 6.7 }}] {Liling Yang. et al.Liang-Ge-San attenuates virus-induced acute lung injury by targeting FXR-mediated ACE2 downregulation to modulate the formation of the cytokine storm..PHYTOMEDICINE.2025 May:140:156584.} {IF} {}
[IF={{ 4.414 }}] {Liu Y et al. Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD. J Nutr Biochem. 2019 Apr 4;69:53-62.} {WB} {Mouse}
[IF={{ 3.628 }}] {Jing Zhao. et al. The choleretic role of tauroursodeoxycholic acid exacerbates alpha-naphthylisothiocyanate induced cholestatic liver injury through the FXR/BSEP pathway. J APPL TOXICOL. 2023 Feb;:} {WB} {Mouse}
[IF={{ 3.5 }}] {Xiliang Du. et al. Expression of hepatic genes involved in bile acid metabolism in dairy cows with fatty liver. J DAIRY SCI. 2024 May;:} {WB} {Bovine}
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NR0B2 Rabbit pAb(bs-4311R)-50ul/100ul/200ul
￥1180 - 2800
