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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8556R |
| 英文名称 | GNAI2/G protein alpha inhibitor 1 Rabbit pAb |
| 中文名称 | G蛋白α抑制蛋白1抗体 |
| 英文别名 | G alpha i-1; G Protein alpha Inhibitor 1; Adenylate cyclase inhibiting G alpha; Gi; Gi1 protein alpha subunit; Gi2 protein alpha subunit; GIP; GNAI1; GNAI2; GNAI2B; Guanine nucleotide binding protein G(i) alpha 1 subunit; Guanine nucleotide binding protein G(i) alpha 2 subunit; GNAI2_HUMAN; Adenylate cyclase inhibiting G alpha protein; Adenylate cyclase-inhibiting G alpha protein; GNAI 2; Gnai2; GTP binding regulatory protein Gi alpha 2 chain; Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2; Guanine nucleotide-binding protein G(i) subunit alpha-2; H LUCA15.1; H LUCA16.1; WUGSC:H LUCA15.1; WUGSC:H LUCA16.1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human G Protein alpha Inhibitor 1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 40 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Signaling Pathway > G Protein Signaling > Heterotrimeric G Proteins > G Proteins |
| 亚基 | G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1. Interacts with RGS12 and RGS14. Interacts with UNC5B. |
| 亚细胞定位 | Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Cell membrane. Note=Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. |
| 相似性 | Belongs to the G-alpha family. G(i/o/t/z) subfamily. |
| 功能 | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. Isoform sGi2: Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The G protein family of signal transducers includes 5 heterotrimers, which are most clearly distinguished by their different alpha chains; they have virtually identical beta chains and similar gamma chains. G protein alpha inhibitor chains 1 and 2 are subunits of the G(i) G protein, whose role is in inhibiting adenylate cyclase activity in response to bound GTP. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验[IF={{ 2.4 }}] {Afifiyan, N., et al. "The role of Tec kinase signaling pathways in the development of Mallory Denk Bodies in balloon cells in alcoholic hepatitis." Experimental and Molecular Pathology (2017)} {IHC} {="Human"}
Protein purification: tropomyosin
Overview TROPOMYOSIN An ubiquitous actin binding protein arranged in alpha-helical coiled-coil of 410 Angstrom length
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
‐88. Ernst, V., Levin, D.H., Foulkes, J.G., and London, I.M. 1982. Effects of skeletal muscle protein phosphatase inhibitor‐2 on protein synthesis and protein
Protein purification: skeletal muscle myosin
consists of an alpha-helical coiled-coil rod of 150 nm in length. The most important mechano-enzyme for muscle contraction. Material Source: rabbit skeletal muscle
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