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- bs-7808R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7808R |
| 英文名称 | KNTC1 Rabbit pAb |
| 中文名称 | 着丝点关联蛋白1抗体 |
| 英文别名 | HsROD; Kinetochore associated 1; KNTC1; ROD; Rough Deal homolog, centromere/kinetochore protein; KNTC1_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human KNTC1/Kinetochore |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 251 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Interacts with ZW10. This interaction is required forstable association with the kinetochore. Component of the RZZcomplex composed of KNTC1/ROD, ZW10 and ZWILCH. |
| 亚细胞定位 | Cytoplasm. Nucleus. Chromosome, centromere,kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Dynamic patternof localization during the cell cycle. At interphase, uniformlydistributed throughout the cytoplasm and nucleus. By prophase anduntil late stages of prometaphase, a fraction of the total pool isconcentrated at kinetochores. By metaphase, detected atkinetochores, along spindle fibers and most prominently at thepoles. By late anaphase until the end of telophase, no longerdetectable on kinetochores or along spindle fibers, but stillpresent at the spindle poles. |
| 组织特异性 | High expression in testis. |
| 功能 | Essential component of the mitotic checkpoint, whichprevents cells from prematurely exiting mitosis. Required for theassembly of the dynein-dynactin and MAD1-MAD2 complexes ontokinetochores. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The kinetochore is the protein structure in eukaryotes which assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis. The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA; and an outer kinetochore, which interacts with microtubules. Even the simplest kinetochores consist of more than 45 different proteins, many of which are conserved throughout eukaryote species. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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