Sample:
_x000D_
NIH/3T3(Mouse) Cell Lysate at 30 ug
_x000D_
Primary: Anti-phospho-GATA4 (Ser262) (bs-6879R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 49 kD
_x000D_
Observed band size: 49 kD
- ￥1180 - 2800
- Bioss
- bs-6879R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6879R |
| 英文名称 | phospho-GATA4 (Ser262) Rabbit pAb |
| 中文名称 | 磷酸化GATA结合蛋白4抗体 |
| 英文别名 | GATA4(phospho S262); p-GATA-4(Ser 262); p-GATA-4(Ser262); GATA binding factor 4; GATA 4; GATA4; GATA-4; Transcription factor GATA-4; GATA-binding factor 4; Gata4; GATA4_HUMAN; GATA-binding factor 4. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Chicken, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human GATA4 around the phosphorylation site of Ser262 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 49 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Tumor suppressors Cardiovascular > Cardiovascular Markers Cardiovascular > Cardiovascular Markers > Cell Markers > Cardiomyocytes Cardiovascular > Heart > Hypertrophy > Transcription factors Developmental Biology > Lineage specification > Endoderm Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Stem Cells > Lineage Markers > Endoderm Stem Cells > Mesenchymal Stem Cells > Myogenesis |
| 亚基 | Interacts with ZNF260. Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1. Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes._x000D_ |
| 亚细胞定位 | Nucleus._x000D_ |
| 翻译后修饰 | Methylation at Lys-300 attenuates transcriptional activity._x000D_ |
| 相似性 | Contains 2 GATA-type zinc fingers._x000D_ |
| 功能 | Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement._x000D_ |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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GATA4 (p-S262) Rabbit pAb(bs-6879R)-50ul/100ul/200ul
￥1180 - 2800
