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- bs-5234R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5234R |
| 英文名称 | phospho-Bcl-xL (Thr115) Rabbit pAb |
| 中文名称 | 磷酸化Bcl-xL蛋白抗体 |
| 英文别名 | BCL2L1(phospho T115); BCL2L1(phospho Thr115); p-Bcl-xL(Thr115); p-BCL2L1(Thr115); Apoptosis regulator Bcl X; BclX; Bcl-X; Bcl 2 like 1; Bcl 2 like 1 protein; Bcl xL; BCL X/L; BCL XL/S; Bcl xS; Bcl2 like1; BCL2-related gene; BCL2-related protein, long isoform, included; BCLXL, included; BCL2-related protein, short isoform, included; BCLXS, included; BCL2L; Bclx; DKFZp781P2092; MGC113803; MGC99998; B2CL1_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human BCL2L1 around the phosphorylation site of Thr115 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 26 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cancer > Cell Death > Apoptosis > Apoptosis Markers > Bcl 2 family Cancer > Cell Death > Apoptosis > Metabolism Cancer > Invasion/microenvironment > Apoptosis > Bcl 2 family Cancer > Oncoproteins/suppressors > Oncoproteins > Cell survival & death Cancer > Tumor biomarkers > Oncoproteins Cell Biology > Apoptosis > Intracellular > Bcl2 Family Metabolism > Pathways and Processes > Metabolism processes > Apoptosis Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Mitochondrial |
| 亚基 | Homodimer. Isoform Bcl-X(L) forms heterodimers with BAX, BAK or BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Interacts with BCL2L11. Interacts with DMN1L; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with BAD and BBC3. Interacts (isoform Bcl-X(L)) with SIVA1 (isoform 1); the interaction inhibits the anti-apoptotic activity. Interacts with BECN1 and PGAM5. Interacts (isoform Bcl-X(L)) with BAX (isoform Sigma). Isoform Bcl-X(L) interacts with IKZF3. Interacts with HEBP2. |
| 亚细胞定位 | Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, centrosome. Note=Mitochondrial membranes and perinuclear envelope. Localizes to the centrosome when phosphorylated at Ser-49. |
| 组织特异性 | Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. |
| 翻译后修饰 | Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. |
| 相似性 | Belongs to the Bcl-2 family. |
| 功能 | Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | bs-1336P is one synthetic peptide derived from human Bcl-xL. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Independently Validated Antibody, image provided by Science Direct, badge number 029725:Formalin-fixed and paraffin embedded human prostate cancer labeled with Rabbit Anti-phospho-Bcl-xL (Thr115) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:250 overnight at 4C followed by conjugation to the secondary antibody Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody for two hours and DAB staining
Independently Validated Antibody, image provided by Science Direct, badge number 029725:Formalin-fixed and paraffin embedded human ovary labeled with Rabbit Anti-phospho-Bcl-xL (Thr115) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:250 overnight at 4C followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-xL (Thr115)) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Bcl-xL (Thr115)) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouset brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Bcl-xL (Thr115)) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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phospho-Bcl-xL (Thr115) Rabbit pAb(bs-5234R)-50ul/100ul/200ul
￥1180 - 2800
