- 询价
- ExCell Bio
- 中国
- CS012-0066
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
ExCell Bio
- 规格：
8 tubes/strip, 125 strips/box, 10 boxs/CS
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文献和实验36 x 0.2ml Standard Micro-tubes 72 x 0.1ml Strip tubes Heating/Cooling Rate: 2.5°C/second (tube temperature) 5.0°C/second (air temperature
culture tubes w/ an appropriate selection media for your plasmid of interest, label 1.5mL tubes and place inside culture tube as a cap.Prepare PCR master mix: buffer, MgCl2, dNTPs, primers, sucrose red, and enzyme- Aliquot 25µL of master mix into a PCR
Inverse PCR for PAC-end sequencing
In strip tubes: 2.5ul Big Dye Mix 1ul Template DNA 1ul primer (one of the primers used for amplification) 3.5ul H20 cap Spin down 10" to get all products mixed at bottom Cycle sequencing - 35 cycles 94C - 15" 45C - 5" 72C
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