pIC7

名称	编号	规格	价格	手册
pIC7

质粒编号:
质粒名称: pIC7
其他名称:
质粒大小（bp）: 2668
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: L08880
其他属性: These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled from pUC8 and pIC7 by F. Pfeiffer For construction of pIC7, a synthetic oligonucleotide has been used to replace the pUC8 polylinker and thus to construct a new cloning vector with a different polylinker. The other pIC-vectors are based on this new pIC7 polylinker, which was combined with the existing pUC9 and pUC19 polylinkers in the following arrangements: pIC19 and pICEM19 vectors: pIC19R: EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI pIC19H: HindIII- Poly (pUC9) -EcoRI – Poly (pIC7) -HindIII pIC20-vectors: pIC20R: EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI pIC20H: HindIII- Poly(pUC19) -EcoRI – Poly (pIC7) -HindIII To produce greater versatility of insertional inactivation of beta-galactosidase activity for subcloning and sequencing, a chemically synthesized oligonucleotide, specifying nine restriction sites including BglII, XhoI, NruI, ClaI, SacI and EcorV in various configurations with existing polylinkers, was created. These improved polylinkers were inserted into plasmids for routine cloning of ds-DNA and into chimeric phage/plasmids for biological production of ss-DNA. The most versatile polyrecognition pattern specifies 17 restriction sites in the beta-galactosidase alpha-complementing gene fragment. Clone pIC7 was used to produce all the other polylinker-carrying vectors. A general purpose cloning vector for inserting DNA into polylinker in lacZ'. Medium is 1227 LB plus ampicillin. Hosts: E.coli TB1, E.coli. Related vectors: pIC7, pUC8.
质粒图谱:

质粒序列:

1	gcgcccaata	cgcaaaccgc	ctctccccgc	gcgttggccg	attcattaat	50
51	gcagctggca	cgacaggttt	cccgactgga	aagcgggcag	tgagcgcaac	100
101	gcaattaatg	tgagttagct	cactcattag	gcaccccagg	ctttacactt	150
151	tatgcttccg	gctcgtatgt	tgtgtggaat	tgtgagcgga	taacaatttc	200
201	acacaggaaa	cagctatgac	catgattacg	aattcatcga	tatctagatc	250
251	tcgagctcgc	gaaagcttgg	cactggccgt	cgttttacaa	cgtcgtgact	300
301	gggaaaaccc	tggcgttacc	caacttaatc	gccttgcagc	acatccccct	350
351	ttcgccagct	ggcgtaatag	cgaagaggcc	cgcaccgatc	gcccttccca	400
401	acagttgcgc	agcctgaatg	gcgaatggcg	cctgatgcgg	tattttctcc	450
451	ttacgcatct	gtgcggtatt	tcacaccgca	tatggtgcac	tctcagtaca	500
501	atctgctctg	atgccgcata	gttaagccag	ccccgacacc	cgccaacacc	550
551	cgctgacgcg	ccctgacggg	cttgtctgct	cccggcatcc	gcttacagac	600
601	aagctgtgac	cgtctccggg	agctgcatgt	gtcagaggtt	ttcaccgtca	650
651	tcaccgaaac	gcgcgagacg	aaagggcctc	gtgatacgcc	tatttttata	700
701	ggttaatgtc	atgataataa	tggtttctta	gacgtcaggt	ggcacttttc	750
751	ggggaaatgt	gcgcggaacc	cctatttgtt	tatttttcta	aatacattca	800
801	aatatgtatc	cgctcatgag	acaataaccc	tgataaatgc	ttcaataata	850
851	ttgaaaaagg	aagagtatga	gtattcaaca	tttccgtgtc	gcccttattc	900
901	ccttttttgc	ggcattttgc	cttcctgttt	ttgctcaccc	agaaacgctg	950
951	gtgaaagtaa	aagatgctga	agatcagttg	ggtgcacgag	tgggttacat	1000
1001	cgaactggat	ctcaacagcg	gtaagatcct	tgagagtttt	cgccccgaag	1050
1051	aacgttttcc	aatgatgagc	acttttaaag	ttctgctatg	tggcgcggta	1100
1101	ttatcccgta	ttgacgccgg	gcaagagcaa	ctcggtcgcc	gcatacacta	1150
1151	ttctcagaat	gacttggttg	agtactcacc	agtcacagaa	aagcatctta	1200
1201	cggatggcat	gacagtaaga	gaattatgca	gtgctgccat	aaccatgagt	1250
1251	gataacactg	cggccaactt	acttctgaca	acgatcggag	gaccgaagga	1300
1301	gctaaccgct	tttttgcaca	acatggggga	tcatgtaact	cgccttgatc	1350
1351	gttgggaacc	ggagctgaat	gaagccatac	caaacgacga	gcgtgacacc	1400
1401	acgatgcctg	tagcaatggc	aacaacgttg	cgcaaactat	taactggcga	1450
1451	actacttact	ctagcttccc	ggcaacaatt	aatagactgg	atggaggcgg	1500
1501	ataaagttgc	aggaccactt	ctgcgctcgg	cccttccggc	tggctggttt	1550
1551	attgctgata	aatctggagc	cggtgagcgt	gggtctcgcg	gtatcattgc	1600
1601	agcactgggg	ccagatggta	agccctcccg	tatcgtagtt	atctacacga	1650
1651	cggggagtca	ggcaactatg	gatgaacgaa	atagacagat	cgctgagata	1700
1701	ggtgcctcac	tgattaagca	ttggtaactg	tcagaccaag	tttactcata	1750
1751	tatactttag	attgatttaa	aacttcattt	ttaatttaaa	aggatctagg	1800
1801	tgaagatcct	ttttgataat	ctcatgacca	aaatccctta	acgtgagttt	1850
1851	tcgttccact	gagcgtcaga	ccccgtagaa	aagatcaaag	gatcttcttg	1900
1901	agatcctttt	tttctgcgcg	taatctgctg	cttgcaaaca	aaaaaaccac	1950
1951	cgctaccagc	ggtggtttgt	ttgccggatc	aagagctacc	aactcttttt	2000
2001	ccgaaggtaa	ctggcttcag	cagagcgcag	ataccaaata	ctgtccttct	2050
2051	agtgtagccg	tagttaggcc	accacttcaa	gaactctgta	gcaccgccta	2100
2101	catacctcgc	tctgctaatc	ctgttaccag	tggctgctgc	cagtggcgat	2150
2151	aagtcgtgtc	ttaccgggtt	ggactcaaga	cgatagttac	cggataaggc	2200
2201	gcagcggtcg	ggctgaacgg	ggggttcgtg	cacacagccc	agcttggagc	2250
2251	gaacgaccta	caccgaactg	agatacctac	agcgtgagct	atgagaaagc	2300
2301	gccacgcttc	ccgaagggag	aaaggcggac	aggtatccgg	taagcggcag	2350
2351	ggtcggaaca	ggagagcgca	cgagggagct	tccaggggga	aacgcctggt	2400
2401	atctttatag	tcctgtcggg	tttcgccacc	tctgacttga	gcgtcgattt	2450
2451	ttgtgatgct	cgtcaggggg	gcggagccta	tggaaaaacg	ccagcaacgc	2500
2501	ggccttttta	cggttcctgg	ccttttgctg	gccttttgct	cacatgttct	2550
2551	ttcctgcgtt	atcccctgat	tctgtggata	accgtattac	cgcctttgag	2600
2601	tgagctgata	ccgctcgccg	cagccgaacg	accgagcgca	gcgagtcagt	2650
2651	gagcgaggaa	gcggaaga	2668