pIC19R

名称	编号	规格	价格	手册
pIC19R

质粒编号:
质粒名称: pIC19R
其他名称:
质粒大小（bp）: 2695
抗性: Ampicillin.
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: L08911
其他属性: These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled from pUC9 and pIC7 by F. Pfeiffer For construction of pIC7, a synthetic oligonucleotide has been used to replace the pUC8 polylinker and thus to construct a new cloning vector with a different polylinker. The other pIC-vectors are based on this new pIC7 polylinker, which was combined with the existing pUC9 and pUC19 polylinkers in the following arrangements: pIC19 and pICEM19 vectors: pIC19R: EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI pIC19H: HindIII- Poly (pUC9) -EcoRI – Poly (pIC7) -HindIII pIC20-vectors: pIC20R: EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI pIC20H: HindIII- Poly(pUC19) -EcoRI – Poly (pIC7) -HindIII To produce greater versatility of insertional inactivation of beta-galactosidase activity for subcloning and sequencing, a chemically synthesized oligonucleotide, specifying nine restriction sites including BglII, XhoI, NruI, ClaI, SacI and EcorV in various configurations with existing polylinkers, was created. These improved polylinkers were inserted into plasmids for routine cloning of ds-DNA and into chimeric phage/plasmids for biological production of ss-DNA. The most versatile polyrecognition pattern specifies 17 restriction sites in the beta-galactosidase alpha-complementing gene fragment. Clone pIC7 was used to produce all the other polylinker-carrying vectors. A general purpose plasmid vector with polylinker containing 13 sites in lacZ'. Medium is 1227 LB plus ampicillin. Hosts: E.coli TB1, E.coli. Related vectors: pIC7, pUC9, pIC19H
质粒图谱:

质粒序列:

1	gcgcccaata	cgcaaaccgc	ctctccccgc	gcgttggccg	attcattaat	50
51	gcagctggca	cgacaggttt	cccgactgga	aagcgggcag	tgagcgcaac	100
101	gcaattaatg	tgagttagct	cactcattag	gcaccccagg	ctttacactt	150
151	tatgcttccg	gctcgtatgt	tgtgtggaat	tgtgagcgga	taacaatttc	200
201	acacaggaaa	cagctatgac	catgattacg	aattcatcga	tatctagatc	250
251	tcgagctcgc	gaaagcttgg	ctgcaggtcg	acggatcccc	gggaattcac	300
301	tggccgtcgt	tttacaacgt	cgtgactggg	aaaaccctgg	cgttacccaa	350
351	cttaatcgcc	ttgcagcaca	tccccctttc	gccagctggc	gtaatagcga	400
401	agaggcccgc	accgatcgcc	cttcccaaca	gttgcgcagc	ctgaatggcg	450
451	aatggcgcct	gatgcggtat	tttctcctta	cgcatctgtg	cggtatttca	500
501	caccgcatat	ggtgcactct	cagtacaatc	tgctctgatg	ccgcatagtt	550
551	aagccagccc	cgacacccgc	caacacccgc	tgacgcgccc	tgacgggctt	600
601	gtctgctccc	ggcatccgct	tacagacaag	ctgtgaccgt	ctccgggagc	650
651	tgcatgtgtc	agaggttttc	accgtcatca	ccgaaacgcg	cgagacgaaa	700
701	gggcctcgtg	atacgcctat	ttttataggt	taatgtcatg	ataataatgg	750
751	tttcttagac	gtcaggtggc	acttttcggg	gaaatgtgcg	cggaacccct	800
801	atttgtttat	ttttctaaat	acattcaaat	atgtatccgc	tcatgagaca	850
851	ataaccctga	taaatgcttc	aataatattg	aaaaaggaag	agtatgagta	900
901	ttcaacattt	ccgtgtcgcc	cttattccct	tttttgcggc	attttgcctt	950
951	cctgtttttg	ctcacccaga	aacgctggtg	aaagtaaaag	atgctgaaga	1000
1001	tcagttgggt	gcacgagtgg	gttacatcga	actggatctc	aacagcggta	1050
1051	agatccttga	gagttttcgc	cccgaagaac	gttttccaat	gatgagcact	1100
1101	tttaaagttc	tgctatgtgg	cgcggtatta	tcccgtattg	acgccgggca	1150
1151	agagcaactc	ggtcgccgca	tacactattc	tcagaatgac	ttggttgagt	1200
1201	actcaccagt	cacagaaaag	catcttacgg	atggcatgac	agtaagagaa	1250
1251	ttatgcagtg	ctgccataac	catgagtgat	aacactgcgg	ccaacttact	1300
1301	tctgacaacg	atcggaggac	cgaaggagct	aaccgctttt	ttgcacaaca	1350
1351	tgggggatca	tgtaactcgc	cttgatcgtt	gggaaccgga	gctgaatgaa	1400
1401	gccataccaa	acgacgagcg	tgacaccacg	atgcctgtag	caatggcaac	1450
1451	aacgttgcgc	aaactattaa	ctggcgaact	acttactcta	gcttcccggc	1500
1501	aacaattaat	agactggatg	gaggcggata	aagttgcagg	accacttctg	1550
1551	cgctcggccc	ttccggctgg	ctggtttatt	gctgataaat	ctggagccgg	1600
1601	tgagcgtggg	tctcgcggta	tcattgcagc	actggggcca	gatggtaagc	1650
1651	cctcccgtat	cgtagttatc	tacacgacgg	ggagtcaggc	aactatggat	1700
1701	gaacgaaata	gacagatcgc	tgagataggt	gcctcactga	ttaagcattg	1750
1751	gtaactgtca	gaccaagttt	actcatatat	actttagatt	gatttaaaac	1800
1801	ttcattttta	atttaaaagg	atctaggtga	agatcctttt	tgataatctc	1850
1851	atgaccaaaa	tcccttaacg	tgagttttcg	ttccactgag	cgtcagaccc	1900
1901	cgtagaaaag	atcaaaggat	cttcttgaga	tccttttttt	ctgcgcgtaa	1950
1951	tctgctgctt	gcaaacaaaa	aaaccaccgc	taccagcggt	ggtttgtttg	2000
2001	ccggatcaag	agctaccaac	tctttttccg	aaggtaactg	gcttcagcag	2050
2051	agcgcagata	ccaaatactg	tccttctagt	gtagccgtag	ttaggccacc	2100
2101	acttcaagaa	ctctgtagca	ccgcctacat	acctcgctct	gctaatcctg	2150
2151	ttaccagtgg	ctgctgccag	tggcgataag	tcgtgtctta	ccgggttgga	2200
2201	ctcaagacga	tagttaccgg	ataaggcgca	gcggtcgggc	tgaacggggg	2250
2251	gttcgtgcac	acagcccagc	ttggagcgaa	cgacctacac	cgaactgaga	2300
2301	tacctacagc	gtgagctatg	agaaagcgcc	acgcttcccg	aagggagaaa	2350
2351	ggcggacagg	tatccggtaa	gcggcagggt	cggaacagga	gagcgcacga	2400
2401	gggagcttcc	agggggaaac	gcctggtatc	tttatagtcc	tgtcgggttt	2450
2451	cgccacctct	gacttgagcg	tcgatttttg	tgatgctcgt	caggggggcg	2500
2501	gagcctatgg	aaaaacgcca	gcaacgcggc	ctttttacgg	ttcctggcct	2550
2551	tttgctggcc	ttttgctcac	atgttctttc	ctgcgttatc	ccctgattct	2600
2601	gtggataacc	gtattaccgc	ctttgagtga	gctgataccg	ctcgccgcag	2650
2651	ccgaacgacc	gagcgcagcg	agtcagtgag	cgaggaagcg	gaaga	2695