pFL64-

名称	编号	规格	价格	手册
pFL64-

质粒编号:
质粒名称: pFL64-
其他名称:
质粒大小（bp）: 4763
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: X70273
其他属性: pFL64- was constructed from pGEM-3Zf(-) (Promega) and pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site and the yeast replication origin in the ClaI site. The 1.4 kb BglI fragment of the pGEM3Zf- plasmid bearing the 0.45 kb phage f1 region was used to replace the corresponding BglI fragment in pFL44. The order of the major features in this plasmid is:EcoRI/MCS/HindIII – URA3 – 2 micron ori – pMB1 ori – ampR – f1 ori. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA. Restriction digests of the clone give the following sizes (kb): BamHI–4.8; BglII–3.7, 1.1; ClaI–4.2, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 397138 Hosts: E.coli HB101, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pUC19, yeast 2-micron plasmid, pGEM-3Zf-.
质粒图谱:

质粒序列:

1	tcgcgcgttt	cggtgatgac	ggtgaaaacc	tctgacacat	gcagctcccg	50
51	gagacggtca	cagcttgtct	gtaagcggat	gccgggagca	gacaagcccg	100
101	tcagggcgcg	tgagcgggtg	ttggcgggtg	tcggggctgg	cttaactatg	150
151	cggcatcaga	gcagattgta	ctgagagtgc	accatatgcg	gtgtgaaata	200
201	ccgcacagat	gcgtaaggag	aaaataccgc	atcaggccgc	gccctgtagc	250
251	ggcgcattaa	gcgcggcggg	tgtggtggtt	acgcgcagcg	tgaccgctac	300
301	acttgccagc	gccctagcgc	ccgctccttt	cgctttcttc	ccttcctttc	350
351	tcgccacgtt	cgccggcttt	ccccgtcaag	ctctaaatcg	ggggctccct	400
401	ttagggttcc	gatttagtgc	tttacggcac	ctcgacccca	aaaaacttga	450
451	ttagggtgat	ggttcacgta	gtgggccatc	gccctgatag	acggtttttc	500
501	gccctttgac	gttggagtcc	acgttcttta	atagtggact	cttgttccaa	550
551	actggaacaa	cactcaaccc	tatctcggtc	tattcttttg	atttataagg	600
601	gattttgccg	atttcggcct	attggttaaa	atgagctgat	ttaacaaaaa	650
651	tttaacgcga	attttaacaa	aatattaacg	tgccattcgc	cattcaggct	700
701	gcgcaactgt	tgggaagggc	gatcggtgcg	ggcctcttcg	ctattacgcc	750
751	agctggcgaa	agggggatgt	gctgcaaggc	gattaagttg	ggtaacgcca	800
801	gggttttccc	agtcacgacg	ttgtaaaacg	acggccagtg	aattcgagct	850
851	cggtacccgg	ggatcctcta	gagtcgacct	gcaggcatgc	aagcttggcg	900
901	taatcatggt	catagctgtt	tcctgtgtga	aattgttatc	cgctcacaat	950
951	tccacacaac	atacgagccg	gaagcataaa	gtgtaaagcc	tggggtgcct	1000
1001	aatgagtgag	ctaactcaca	ttaattgcgt	tgcgctcact	gcccgctttc	1050
1051	cagtcgggaa	acctgtcgtg	ccagcagatc	tgagcttttt	ctttccaatt	1100
1101	tttttttttt	gtgagtttag	tatacatgca	tttacttata	atacagtttt	1150
1151	ttagttttgc	tggccgcatc	ttctcaaata	tgcttcccag	cctgcttttc	1200
1201	tgtaacgttc	accctctacc	ttagcatccc	ttccctttgc	aaatagtcct	1250
1251	cttccaacaa	taataatgtc	agatcctgta	gagaccacat	catccacggt	1300
1301	tctatactgt	tgacccaatg	cgtctccctt	gtcatctaaa	cccacaccgg	1350
1351	gtgtcataat	caaccaatcg	taaccttcat	ctcttccacc	catgtctctt	1400
1401	tgagcaataa	agccgataac	aaaatctttg	tcgctcttcg	caatgtcaac	1450
1451	agtaccctta	gtatattctc	cagtagatag	ggagcccttg	catgacaatt	1500
1501	ctgctaacat	caaaaggcct	ctaggttcct	ttgttacttc	ttctgccgcc	1550
1551	tgcttcaaac	cgctaacaat	acctgggccc	accacaccgt	gtgcattcgt	1600
1601	aatgtctgcc	cattctgcta	ttctgtatac	acccgcagag	tactgcaatt	1650
1651	tgactgtatt	accaatgtca	gcaaattttc	tgtcttcgaa	gagtaaaaaa	1700
1701	ttgtacttgg	cggataatgc	ctttagcggc	ttaactgtgc	cctccatgga	1750
1751	aaaatcagtc	aagatatcca	catgtgtttt	tagtaaacaa	attttgggac	1800
1801	ctaatgcttc	aactaactcc	agtaattcct	tggtggtacg	aacatccaat	1850
1851	gaagcacaca	agtttgtttg	cttttcgtgc	atgatattaa	atagcttggc	1900
1901	agcaacagga	ctaggatgag	tagcagcacg	ttccttatat	gtagctttcg	1950
1951	acatgattta	tcttcgtttc	cggtttttgt	tctgtgcagt	tgggttaaga	2000
2001	atactgggca	atttcatgtt	tcttcaacac	tacatatgcg	tatatatacc	2050
2051	aatctaagtc	tgtgctcctt	ccttcgttct	tccttctgtt	cggagattac	2100
2101	cgaatcaaaa	aaatttcaaa	gaaaccgaaa	tcaaaaaaaa	gaataaaaaa	2150
2151	aaaatgatga	attgaaaagc	cagatctgct	gcattaatga	atcggccaac	2200
2201	gcgcggggag	aggcggtttg	cgtattgggc	gctcttccgc	ttcctcgctc	2250
2251	actgactcgc	tgcgctcggt	cgttcggctg	cggcgagcgg	tatcagcatc	2300
2301	gatgcatact	ttgtgaacag	aaagtgatag	cgttgatgat	tcttcattgg	2350
2351	tcagaaaatt	atgaacggtt	tcttctattt	tgtctctata	tactacgtat	2400
2401	aggaaatgtt	tacattttcg	tattgttttc	gattcactct	atgaatagtt	2450
2451	cttactacaa	tttttttgtc	taaagagtaa	tactagagat	aaacataaaa	2500
2501	aatgtagagg	tcgagtttag	atgcaagttc	aaggagcgaa	aggtggatgg	2550
2551	gtaggttata	tagggatata	gcacagagat	atatagcaaa	gagatacttt	2600
2601	tgagcaatgt	ttgtggaagc	ggtattcgca	atattttagt	agctcgttac	2650
2651	agtccggtgc	gtttttggtt	ttttgaaagt	gcgtcttcag	agcgcttttg	2700
2701	gttttcaaaa	gcgctctgaa	gttcctatac	tttctagcta	gagaatagga	2750
2751	acttcggaat	aggaacttca	aagcgtttcc	gaaaacgagc	gcttccgaaa	2800
2801	atgcaacgcg	agctgccatc	gatgctcact	caaaggtcgg	taatacggtt	2850
2851	atccacagaa	tcaggggata	acgcaggaaa	gaacatgtga	gcaaaaggcc	2900
2901	agcaaaaggc	caggaaccgt	aaaaaggccg	cgttgctggc	gtttttccat	2950
2951	aggctccgcc	cccctgacga	gcatcacaaa	aatcgacgct	caagtcagag	3000
3001	gtggcgaaac	ccgacaggac	tataaagata	ccaggcgttt	ccccctggaa	3050
3051	gctccctcgt	gcgctctcct	gttccgaccc	tgccgcttac	cggatacctg	3100
3101	tccgcctttc	tcccttcggg	aagcgtggcg	ctttctcata	gctcacgctg	3150
3151	taggtatctc	agttcggtgt	aggtcgttcg	ctccaagctg	ggctgtgtgc	3200
3201	acgaaccccc	cgttcagccc	gaccgctgcg	ccttatccgg	taactatcgt	3250
3251	cttgagtcca	acccggtaag	acacgactta	tcgccactgg	cagcagccac	3300
3301	tggtaacagg	attagcagag	cgaggtatgt	aggcggtgct	acagagttct	3350
3351	tgaagtggtg	gcctaactac	ggctacacta	gaaggacagt	atttggtatc	3400
3401	tgcgctctgc	tgaagccagt	taccttcgga	aaaagagttg	gtagctcttg	3450
3451	atccggcaaa	caaaccaccg	ctggtagcgg	tggttttttt	gtttgcaagc	3500
3501	agcagattac	gcgcagaaaa	aaaggatctc	aagaagatcc	tttgatcttt	3550
3551	tctacggggt	ctgacgctca	gtggaacgaa	aactcacgtt	aagggatttt	3600
3601	ggtcatgaga	ttatcaaaaa	ggatcttcac	ctagatcctt	ttaaattaaa	3650
3651	aatgaagttt	taaatcaatc	taaagtatat	atgagtaaac	ttggtctgac	3700
3701	agttaccaat	gcttaatcag	tgaggcacct	atctcagcga	tctgtctatt	3750
3751	tcgttcatcc	atagttgcct	gactccccgt	cgtgtagata	actacgatac	3800
3801	gggagggctt	accatctggc	cccagtgctg	caatgatacc	gcgagaccca	3850
3851	cgctcaccgg	ctccagattt	atcagcaata	aaccagccag	ccggaagggc	3900
3901	cgagcgcaga	agtggtcctg	caactttatc	cgcctccatc	cagtctatta	3950
3951	attgttgccg	ggaagctaga	gtaagtagtt	cgccagttaa	tagtttgcgc	4000
4001	aacgttgttg	gcattgctac	aggcatcgtg	gtgtcacgct	cgtcgtttgg	4050
4051	tatggcttca	ttcagctccg	gttcccaacg	atcaaggcga	gttacatgat	4100
4101	cccccatgtt	gtgcaaaaaa	gcggttagct	ccttcggtcc	tccgatcgtt	4150
4151	gtcagaagta	agttggccgc	agtgttatca	ctcatggtta	tggcagcact	4200
4201	gcataattct	cttactgtca	tgccatcggt	aagatgcttt	tctgtgactg	4250
4251	gtgagtactc	aaccaagtca	ttctgagaat	agtgtatgcg	gcgaccgagt	4300
4301	tgctcttgcc	cggcgtcaat	acgggataat	agtgtatgac	atagcagaac	4350
4351	tttaaaagtg	ctcatcattg	gaaaacgttc	ttcggggcga	aaactctcaa	4400
4401	ggatcttacc	gctgttgaga	tccagttcga	tgtaacccac	tcgtgcaccc	4450
4451	aactgatctt	cagcatcttt	tactttcacc	agcgtttctg	ggtgagcaaa	4500
4501	aacaggaagg	caaaatgccg	caaaaaaggg	aataagggcg	acacggaaat	4550
4551	gttgaatact	catactcttc	ctttttcaat	attattgaag	catttatcag	4600
4601	ggttattgtc	tcatgagcgg	atacatattt	gaatgtattt	agaaaaataa	4650
4651	acaaataggg	gttccgcgca	catttccccg	aaaagtgcca	cctgacgtct	4700
4701	aagaaaccat	tattatcatg	acattaacct	ataaaaatag	gcgtatcacg	4750
4751	aggccctttc	gtc	4763