pET-3a

名称	编号	规格	价格	手册
pET-3a(pET3a)(60908-2758）	60908-2758

质粒编号: 60908-2758
质粒名称: pET-3a
其他名称: pET3a
质粒大小（bp）: 4640
抗性: Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: 非病毒
启动子: T7
稳转或瞬转: 瞬时表达
组成型或诱导性: 组成型
表达水平: 高
蛋白标签: 无
5’测序引物: T7

5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性:

质粒图谱:

质粒序列:

1	TAATACGACT	CACTATAGGG	AGACCACAAC	GGTTTCCCTC	TAGAAATAAT	50
51	TTTGTTTAAC	TTTAAGAAGG	AGATATACAT	ATGGCTAGCA	TGACTGGTGG	100
101	ACAGCAAATG	GGTCGCGGAT	CCGGCTGCTA	ACAAAGCCCG	AAAGGAAGCT	150
151	GAGTTGGCTG	CTGCCACCGC	TGAGCAATAA	CTAGCATAAC	CCCTTGGGGC	200
201	CTCTAAACGG	GTCTTGAGGG	GTTTTTTGCT	GAAAGGAGGA	ACTATATCCG	250
251	GATATCCACA	GGACGGGTGT	GGTCGCCATG	ATCGCGTAGT	CGATAGTGGC	300
301	TCCAAGTAGC	GAAGCGAGCA	GGACTGGGCG	GCGGCCAAAG	CGGTCGGACA	350
351	GTGCTCCGAG	AACGGGTGCG	CATAGAAATT	GCATCAACGC	ATATAGCGCT	400
401	AGCAGCACGC	CATAGTGACT	GGCGATGCTG	TCGGAATGGA	CGATATCCCG	450
451	CAAGAGGCCC	GGCAGTACCG	GCATAACCAA	GCCTATGCCT	ACAGCATCCA	500
501	GGGTGACGGT	GCCGAGGATG	ACGATGAGCG	CATTGTTAGA	TTTCATACAC	550
551	GGTGCCTGAC	TGCGTTAGCA	ATTTAACTGT	GATAAACTAC	CGCATTAAAG	600
601	CTTATCGATG	ATAAGCTGTC	AAACATGAGA	ATTCTTGAAG	ACGAAAGGGC	650
651	CTCGTGATAC	GCCTATTTTT	ATAGGTTAAT	GTCATGATAA	TAATGGTTTC	700
701	TTAGACGTCA	GGTGGCACTT	TTCGGGGAAA	TGTGCGCGGA	ACCCCTATTT	750
751	GTTTATTTTT	CTAAATACAT	TCAAATATGT	ATCCGCTCAT	GAGACAATAA	800
801	CCCTGATAAA	TGCTTCAATA	ATATTGAAAA	AGGAAGAGTA	TGAGTATTCA	850
851	ACATTTCCGT	GTCGCCCTTA	TTCCCTTTTT	TGCGGCATTT	TGCCTTCCTG	900
901	TTTTTGCTCA	CCCAGAAACG	CTGGTGAAAG	TAAAAGATGC	TGAAGATCAG	950
951	TTGGGTGCAC	GAGTGGGTTA	CATCGAACTG	GATCTCAACA	GCGGTAAGAT	1000
1001	CCTTGAGAGT	TTTCGCCCCG	AAGAACGTTT	TCCAATGATG	AGCACTTTTA	1050
1051	AAGTTCTGCT	ATGTGGCGCG	GTATTATCCC	GTGTTGACGC	CGGGCAAGAG	1100
1101	CAACTCGGTC	GCCGCATACA	CTATTCTCAG	AATGACTTGG	TTGAGTACTC	1150
1151	ACCAGTCACA	GAAAAGCATC	TTACGGATGG	CATGACAGTA	AGAGAATTAT	1200
1201	GCAGTGCTGC	CATAACCATG	AGTGATAACA	CTGCGGCCAA	CTTACTTCTG	1250
1251	ACAACGATCG	GAGGACCGAA	GGAGCTAACC	GCTTTTTTGC	ACAACATGGG	1300
1301	GGATCATGTA	ACTCGCCTTG	ATCGTTGGGA	ACCGGAGCTG	AATGAAGCCA	1350
1351	TACCAAACGA	CGAGCGTGAC	ACCACGATGC	CTGCAGCAAT	GGCAACAACG	1400
1401	TTGCGCAAAC	TATTAACTGG	CGAACTACTT	ACTCTAGCTT	CCCGGCAACA	1450
1451	ATTAATAGAC	TGGATGGAGG	CGGATAAAGT	TGCAGGACCA	CTTCTGCGCT	1500
1501	CGGCCCTTCC	GGCTGGCTGG	TTTATTGCTG	ATAAATCTGG	AGCCGGTGAG	1550
1551	CGTGGGTCTC	GCGGTATCAT	TGCAGCACTG	GGGCCAGATG	GTAAGCCCTC	1600
1601	CCGTATCGTA	GTTATCTACA	CGACGGGGAG	TCAGGCAACT	ATGGATGAAC	1650
1651	GAAATAGACA	GATCGCTGAG	ATAGGTGCCT	CACTGATTAA	GCATTGGTAA	1700
1701	CTGTCAGACC	AAGTTTACTC	ATATATACTT	TAGATTGATT	TAAAACTTCA	1750
1751	TTTTTAATTT	AAAAGGATCT	AGGTGAAGAT	CCTTTTTGAT	AATCTCATGA	1800
1801	CCAAAATCCC	TTAACGTGAG	TTTTCGTTCC	ACTGAGCGTC	AGACCCCGTA	1850
1851	GAAAAGATCA	AAGGATCTTC	TTGAGATCCT	TTTTTTCTGC	GCGTAATCTG	1900
1901	CTGCTTGCAA	ACAAAAAAAC	CACCGCTACC	AGCGGTGGTT	TGTTTGCCGG	1950
1951	ATCAAGAGCT	ACCAACTCTT	TTTCCGAAGG	TAACTGGCTT	CAGCAGAGCG	2000
2001	CAGATACCAA	ATACTGTCCT	TCTAGTGTAG	CCGTAGTTAG	GCCACCACTT	2050
2051	CAAGAACTCT	GTAGCACCGC	CTACATACCT	CGCTCTGCTA	ATCCTGTTAC	2100
2101	CAGTGGCTGC	TGCCAGTGGC	GATAAGTCGT	GTCTTACCGG	GTTGGACTCA	2150
2151	AGACGATAGT	TACCGGATAA	GGCGCAGCGG	TCGGGCTGAA	CGGGGGGTTC	2200
2201	GTGCACACAG	CCCAGCTTGG	AGCGAACGAC	CTACACCGAA	CTGAGATACC	2250
2251	TACAGCGTGA	GCTATGAGAA	AGCGCCACGC	TTCCCGAAGG	GAGAAAGGCG	2300
2301	GACAGGTATC	CGGTAAGCGG	CAGGGTCGGA	ACAGGAGAGC	GCACGAGGGA	2350
2351	GCTTCCAGGG	GGAAACGCCT	GGTATCTTTA	TAGTCCTGTC	GGGTTTCGCC	2400
2401	ACCTCTGACT	TGAGCGTCGA	TTTTTGTGAT	GCTCGTCAGG	GGGGCGGAGC	2450
2451	CTATGGAAAA	ACGCCAGCAA	CGCGGCCTTT	TTACGGTTCC	TGGCCTTTTG	2500
2501	CTGGCCTTTT	GCTCACATGT	TCTTTCCTGC	GTTATCCCCT	GATTCTGTGG	2550
2551	ATAACCGTAT	TACCGCCTTT	GAGTGAGCTG	ATACCGCTCG	CCGCAGCCGA	2600
2601	ACGACCGAGC	GCAGCGAGTC	AGTGAGCGAG	GAAGCGGAAG	AGCGCCTGAT	2650
2651	GCGGTATTTT	CTCCTTACGC	ATCTGTGCGG	TATTTCACAC	CGCATATATG	2700
2701	GTGCACTCTC	AGTACAATCT	GCTCTGATGC	CGCATAGTTA	AGCCAGTATA	2750
2751	CACTCCGCTA	TCGCTACGTG	ACTGGGTCAT	GGCTGCGCCC	CGACACCCGC	2800
2801	CAACACCCGC	TGACGCGCCC	TGACGGGCTT	GTCTGCTCCC	GGCATCCGCT	2850
2851	TACAGACAAG	CTGTGACCGT	CTCCGGGAGC	TGCATGTGTC	AGAGGTTTTC	2900
2901	ACCGTCATCA	CCGAAACGCG	CGAGGCAGCT	GCGGTAAAGC	TCATCAGCGT	2950
2951	GGTCGTGAAG	CGATTCACAG	ATGTCTGCCT	GTTCATCCGC	GTCCAGCTCG	3000
3001	TTGAGTTTCT	CCAGAAGCGT	TAATGTCTGG	CTTCTGATAA	AGCGGGCCAT	3050
3051	GTTAAGGGCG	GTTTTTTCCT	GTTTGGTCAC	TGATGCCTCC	GTGTAAGGGG	3100
3101	GATTTCTGTT	CATGGGGGTA	ATGATACCGA	TGAAACGAGA	GAGGATGCTC	3150
3151	ACGATACGGG	TTACTGATGA	TGAACATGCC	CGGTTACTGG	AACGTTGTGA	3200
3201	GGGTAAACAA	CTGGCGGTAT	GGATGCGGCG	GGACCAGAGA	AAAATCACTC	3250
3251	AGGGTCAATG	CCAGCGCTTC	GTTAATACAG	ATGTAGGTGT	TCCACAGGGT	3300
3301	AGCCAGCAGC	ATCCTGCGAT	GCAGATCCGG	AACATAATGG	TGCAGGGCGC	3350
3351	TGACTTCCGC	GTTTCCAGAC	TTTACGAAAC	ACGGAAACCG	AAGACCATTC	3400
3401	ATGTTGTTGC	TCAGGTCGCA	GACGTTTTGC	AGCAGCAGTC	GCTTCACGTT	3450
3451	CGCTCGCGTA	TCGGTGATTC	ATTCTGCTAA	CCAGTAAGGC	AACCCCGCCA	3500
3501	GCCTAGCCGG	GTCCTCAACG	ACAGGAGCAC	GATCATGCGC	ACCCGTGGCC	3550
3551	AGGACCCAAC	GCTGCCCGAG	ATGCGCCGCG	TGCGGCTGCT	GGAGATGGCG	3600
3601	GACGCGATGG	ATATGTTCTG	CCAAGGGTTG	GTTTGCGCAT	TCACAGTTCT	3650
3651	CCGCAAGAAT	TGATTGGCTC	CAATTCTTGG	AGTGGTGAAT	CCGTTAGCGA	3700
3701	GGTGCCGCCG	GCTTCCATTC	AGGTCGAGGT	GGCCCGGCTC	CATGCACCGC	3750
3751	GACGCAACGC	GGGGAGGCAG	ACAAGGTATA	GGGCGGCGCC	TACAATCCAT	3800
3801	GCCAACCCGT	TCCATGTGCT	CGCCGAGGCG	GCATAAATCG	CCGTGACGAT	3850
3851	CAGCGGTCCA	GTGATCGAAG	TTAGGCTGGT	AAGAGCCGCG	AGCGATCCTT	3900
3901	GAAGCTGTCC	CTGATGGTCG	TCATCTACCT	GCCTGGACAG	CATGGCCTGC	3950
3951	AACGCGGGCA	TCCCGATGCC	GCCGGAAGCG	AGAAGAATCA	TAATGGGGAA	4000
4001	GGCCATCCAG	CCTCGCGTCG	CGAACGCCAG	CAAGACGTAG	CCCAGCGCGT	4050
4051	CGGCCGCCAT	GCCGGCGATA	ATGGCCTGCT	TCTCGCCGAA	ACGTTTGGTG	4100
4101	GCGGGACCAG	TGACGAAGGC	TTGAGCGAGG	GCGTGCAAGA	TTCCGAATAC	4150
4151	CGCAAGCGAC	AGGCCGATCA	TCGTCGCGCT	CCAGCGAAAG	CGGTCCTCGC	4200
4201	CGAAAATGAC	CCAGAGCGCT	GCCGGCACCT	GTCCTACGAG	TTGCATGATA	4250
4251	AAGAAGACAG	TCATAAGTGC	GGCGACGATA	GTCATGCCCC	GCGCCCACCG	4300
4301	GAAGGAGCTG	ACTGGGTTGA	AGGCTCTCAA	GGGCATCGGT	CGACGCTCTC	4350
4351	CCTTATGCGA	CTCCTGCATT	AGGAAGCAGC	CCAGTAGTAG	GTTGAGGCCG	4400
4401	TTGAGCACCG	CCGCCGCAAG	GAATGGTGCA	TGCAAGGAGA	TGGCGCCCAA	4450
4451	CAGTCCCCCG	GCCACGGGGC	CTGCCACCAT	ACCCACGCCG	AAACAAGCGC	4500
4501	TCATGAGCCC	GAAGTGGCGA	GCCCGATCTT	CCCCATCGGT	GATGTCGGCG	4550
4551	ATATAGGCGC	CAGCAACCGC	ACCTGTGGCG	CCGGTGATGC	CGGCCACGAT	4600
4601	GCGTCCGGCG	TAGAGGATCG	AGATCTCGAT	CCCGCGAAAT	4640