pET-32Ek/LIC

名称	编号	规格	价格	手册
pET-32Ek/LIC(pET32Ek/LIC)(60908-2799)	60908-2799

质粒编号: 60908-2799
质粒名称: pET-32Ek/LIC
其他名称:
质粒大小（bp）: 5917
抗性: Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: 高
蛋白标签: thioredoxin (N端); His (中间和C端), N-EK
5′ 测序引物:T7或者Trx-F

5′ 测序引物序列: T7: 5′-TAATACGACTCACTATAGGG-3′; Trx-F: 5′ TTCCTCGACGCTAACCTG 3′
GenBank号:
其他属性: For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site.
质粒图谱:

Feature Name	Start	End
T7_terminator	129	1
T7_Terminal_primer	69	87
EK	250	236
S15	310	266
lacI	319	2270
6xHIS	361	344
T7_transl_en_RBS	734	718
lacO	779	752
T7_promoter	797	779
tet (300 – 563)	833	1096
pBRrevBam_primer	904	885
tet (576 – 851)	2329	2604
ROP	3079	3270
pGEX_3_primer	3286	3264
pBR322_origin	4304	3685
Ampicillin	5319	4459
AmpR_promoter	5389	5361

ORF	Start	End
ORF frame 1	709	137
ORF frame 2	344	1438
ORF frame 3	1311	2270
ORF frame 2	5319	4459

Enzyme Name	Cut
XhoI	158
NotI	166
EagI	166
HindIII	173
SalI	179
SacI	190
EcoRI	192
BamHI	198
EcoRV	206
NcoI	211
XmaI	228
SmaI	230
KpnI	255
BglII	258
BstBI	285
MscI	368
XbaI	746
BclI	1552
ApaI	1749
HpaI	2044
PstI	4777

质粒序列:

1	atccggatat	agttcctcct	ttcagcaaaa	aacccctcaa	gacccgttta	50
51	gaggccccaa	ggggttatgc	tagttattgc	tcagcggtgg	cagcagccaa	100
101	ctcagcttcc	tttcgggctt	tgttagcagc	cggatctcag	tggtggtggt	150
151	ggtggtgctc	gagtgcggcc	gcaagcttgt	cgacggagct	cgaattcgga	200
201	tccgatatcg	ccatggttga	ggagaagccc	gggctcttgt	cgtcgtcatc	250
251	ggtacccaga	tctgggctgt	ccatgtgctg	gcgttcgaat	ttagcagcag	300
301	cggtttcttt	cataccagaa	ccgcgtggca	ccagaccaga	agaatgatga	350
351	tgatgatggt	gcatatggcc	agaaccagaa	ccggccaggt	tagcgtcgag	400
401	gaactctttc	aactgacctt	tagacagtgc	acccactttg	gttgccgcca	450
451	cttcaccgtt	tttgaacagc	agcagagtcg	ggataccacg	gatgccatat	500
501	ttcggcgcag	tgccagggtt	ttgatcgatg	ttcagttttg	caacggtcag	550
551	tttgccctga	tattcgtcag	cgatttcatc	cagaatcggg	gcgatcattt	600
601	tgcacggacc	gcaccactct	gcccagaaat	cgacgaggat	cgccccgtcc	650
651	gctttgagta	catccgtgtc	aaaactgtcg	tcagtcaggt	gaataatttt	700
701	atcgctcata	tgtatatctc	cttcttaaag	ttaaacaaaa	ttatttctag	750
751	aggggaattg	ttatccgctc	acaattcccc	tatagtgagt	cgtattaatt	800
801	tcgcgggatc	gagatcgatc	tcgatcctct	acgccggacg	catcgtggcc	850
851	ggcatcaccg	gcgccacagg	tgcggttgct	ggcgcctata	tcgccgacat	900
901	caccgatggg	gaagatcggg	ctcgccactt	cgggctcatg	agcgcttgtt	950
951	tcggcgtggg	tatggtggca	ggccccgtgg	ccgggggact	gttgggcgcc	1000
1001	atctccttgc	atgcaccatt	ccttgcggcg	gcggtgctca	acggcctcaa	1050
1051	cctactactg	ggctgcttcc	taatgcagga	gtcgcataag	ggagagcgtc	1100
1101	gagatcccgg	acaccatcga	atggcgcaaa	acctttcgcg	gtatggcatg	1150
1151	atagcgcccg	gaagagagtc	aattcagggt	ggtgaatgtg	aaaccagtaa	1200
1201	cgttatacga	tgtcgcagag	tatgccggtg	tctcttatca	gaccgtttcc	1250
1251	cgcgtggtga	accaggccag	ccacgtttct	gcgaaaacgc	gggaaaaagt	1300
1301	ggaagcggcg	atggcggagc	tgaattacat	tcccaaccgc	gtggcacaac	1350
1351	aactggcggg	caaacagtcg	ttgctgattg	gcgttgccac	ctccagtctg	1400
1401	gccctgcacg	cgccgtcgca	aattgtcgcg	gcgattaaat	ctcgcgccga	1450
1451	tcaactgggt	gccagcgtgg	tggtgtcgat	ggtagaacga	agcggcgtcg	1500
1501	aagcctgtaa	agcggcggtg	cacaatcttc	tcgcgcaacg	cgtcagtggg	1550
1551	ctgatcatta	actatccgct	ggatgaccag	gatgccattg	ctgtggaagc	1600
1601	tgcctgcact	aatgttccgg	cgttatttct	tgatgtctct	gaccagacac	1650
1651	ccatcaacag	tattattttc	tcccatgaag	acggtacgcg	actgggcgtg	1700
1701	gagcatctgg	tcgcattggg	tcaccagcaa	atcgcgctgt	tagcgggccc	1750
1751	attaagttct	gtctcggcgc	gtctgcgtct	ggctggctgg	cataaatatc	1800
1801	tcactcgcaa	tcaaattcag	ccgatagcgg	aacgggaagg	cgactggagt	1850
1851	gccatgtccg	gttttcaaca	aaccatgcaa	atgctgaatg	agggcatcgt	1900
1901	tcccactgcg	atgctggttg	ccaacgatca	gatggcgctg	ggcgcaatgc	1950
1951	gcgccattac	cgagtccggg	ctgcgcgttg	gtgcggacat	ctcggtagtg	2000
2001	ggatacgacg	ataccgaaga	cagctcatgt	tatatcccgc	cgttaaccac	2050
2051	catcaaacag	gattttcgcc	tgctggggca	aaccagcgtg	gaccgcttgc	2100
2101	tgcaactctc	tcagggccag	gcggtgaagg	gcaatcagct	gttgcccgtc	2150
2151	tcactggtga	aaagaaaaac	caccctggcg	cccaatacgc	aaaccgcctc	2200
2201	tccccgcgcg	ttggccgatt	cattaatgca	gctggcacga	caggtttccc	2250
2251	gactggaaag	cgggcagtga	gcgcaacgca	attaatgtaa	gttagctcac	2300
2301	tcattaggca	ccgggatctc	gaccgatgcc	cttgagagcc	ttcaacccag	2350
2351	tcagctcctt	ccggtgggcg	cggggcatga	ctatcgtcgc	cgcacttatg	2400
2401	actgtcttct	ttatcatgca	actcgtagga	caggtgccgg	cagcgctctg	2450
2451	ggtcattttc	ggcgaggacc	gctttcgctg	gagcgcgacg	atgatcggcc	2500
2501	tgtcgcttgc	ggtattcgga	atcttgcacg	ccctcgctca	agccttcgtc	2550
2551	actggtcccg	ccaccaaacg	tttcggcgag	aagcaggcca	ttatcgccgg	2600
2601	catggcggcc	ccacgggtgc	gcatgatcgt	gctcctgtcg	ttgaggaccc	2650
2651	ggctaggctg	gcggggttgc	cttactggtt	agcagaatga	atcaccgata	2700
2701	cgcgagcgaa	cgtgaagcga	ctgctgctgc	aaaacgtctg	cgacctgagc	2750
2751	aacaacatga	atggtcttcg	gtttccgtgt	ttcgtaaagt	ctggaaacgc	2800
2801	ggaagtcagc	gccctgcacc	attatgttcc	ggatctgcat	cgcaggatgc	2850
2851	tgctggctac	cctgtggaac	acctacatct	gtattaacga	agcgctggca	2900
2901	ttgaccctga	gtgatttttc	tctggtcccg	ccgcatccat	accgccagtt	2950
2951	gtttaccctc	acaacgttcc	agtaaccggg	catgttcatc	atcagtaacc	3000
3001	cgtatcgtga	gcatcctctc	tcgtttcatc	ggtatcatta	cccccatgaa	3050
3051	cagaaatccc	ccttacacgg	aggcatcagt	gaccaaacag	gaaaaaaccg	3100
3101	cccttaacat	ggcccgcttt	atcagaagcc	agacattaac	gcttctggag	3150
3151	aaactcaacg	agctggacgc	ggatgaacag	gcagacatct	gtgaatcgct	3200
3201	tcacgaccac	gctgatgagc	tttaccgcag	ctgcctcgcg	cgtttcggtg	3250
3251	atgacggtga	aaacctctga	cacatgcagc	tcccggagac	ggtcacagct	3300
3301	tgtctgtaag	cggatgccgg	gagcagacaa	gcccgtcagg	gcgcgtcagc	3350
3351	gggtgttggc	gggtgtcggg	gcgcagccat	gacccagtca	cgtagcgata	3400
3401	gcggagtgta	tactggctta	actatgcggc	atcagagcag	attgtactga	3450
3451	gagtgcacca	tatatgcggt	gtgaaatacc	gcacagatgc	gtaaggagaa	3500
3501	aataccgcat	caggcgctct	tccgcttcct	cgctcactga	ctcgctgcgc	3550
3551	tcggtcgttc	ggctgcggcg	agcggtatca	gctcactcaa	aggcggtaat	3600
3601	acggttatcc	acagaatcag	gggataacgc	aggaaagaac	atgtgagcaa	3650
3651	aaggccagca	aaaggccagg	aaccgtaaaa	aggccgcgtt	gctggcgttt	3700
3701	ttccataggc	tccgcccccc	tgacgagcat	cacaaaaatc	gacgctcaag	3750
3751	tcagaggtgg	cgaaacccga	caggactata	aagataccag	gcgtttcccc	3800
3801	ctggaagctc	cctcgtgcgc	tctcctgttc	cgaccctgcc	gcttaccgga	3850
3851	tacctgtccg	cctttctccc	ttcgggaagc	gtggcgcttt	ctcatagctc	3900
3901	acgctgtagg	tatctcagtt	cggtgtaggt	cgttcgctcc	aagctgggct	3950
3951	gtgtgcacga	accccccgtt	cagcccgacc	gctgcgcctt	atccggtaac	4000
4001	tatcgtcttg	agtccaaccc	ggtaagacac	gacttatcgc	cactggcagc	4050
4051	agccactggt	aacaggatta	gcagagcgag	gtatgtaggc	ggtgctacag	4100
4101	agttcttgaa	gtggtggcct	aactacggct	acactagaag	gacagtattt	4150
4151	ggtatctgcg	ctctgctgaa	gccagttacc	ttcggaaaaa	gagttggtag	4200
4201	ctcttgatcc	ggcaaacaaa	ccaccgctgg	tagcggtggt	ttttttgttt	4250
4251	gcaagcagca	gattacgcgc	agaaaaaaag	gatctcaaga	agatcctttg	4300
4301	atcttttcta	cggggtctga	cgctcagtgg	aacgaaaact	cacgttaagg	4350
4351	gattttggtc	atgagattat	caaaaaggat	cttcacctag	atccttttaa	4400
4401	attaaaaatg	aagttttaaa	tcaatctaaa	gtatatatga	gtaaacttgg	4450
4451	tctgacagtt	accaatgctt	aatcagtgag	gcacctatct	cagcgatctg	4500
4501	tctatttcgt	tcatccatag	ttgcctgact	ccccgtcgtg	tagataacta	4550
4551	cgatacggga	gggcttacca	tctggcccca	gtgctgcaat	gataccgcga	4600
4601	gacccacgct	caccggctcc	agatttatca	gcaataaacc	agccagccgg	4650
4651	aagggccgag	cgcagaagtg	gtcctgcaac	tttatccgcc	tccatccagt	4700
4701	ctattaattg	ttgccgggaa	gctagagtaa	gtagttcgcc	agttaatagt	4750
4751	ttgcgcaacg	ttgttgccat	tgctgcaggc	atcgtggtgt	cacgctcgtc	4800
4801	gtttggtatg	gcttcattca	gctccggttc	ccaacgatca	aggcgagtta	4850
4851	catgatcccc	catgttgtgc	aaaaaagcgg	ttagctcctt	cggtcctccg	4900
4901	atcgttgtca	gaagtaagtt	ggccgcagtg	ttatcactca	tggttatggc	4950
4951	agcactgcat	aattctctta	ctgtcatgcc	atccgtaaga	tgcttttctg	5000
5001	tgactggtga	gtactcaacc	aagtcattct	gagaatagtg	tatgcggcga	5050
5051	ccgagttgct	cttgcccggc	gtcaatacgg	gataataccg	cgccacatag	5100
5101	cagaacttta	aaagtgctca	tcattggaaa	acgttcttcg	gggcgaaaac	5150
5151	tctcaaggat	cttaccgctg	ttgagatcca	gttcgatgta	acccactcgt	5200
5201	gcacccaact	gatcttcagc	atcttttact	ttcaccagcg	tttctgggtg	5250
5251	agcaaaaaca	ggaaggcaaa	atgccgcaaa	aaagggaata	agggcgacac	5300
5301	ggaaatgttg	aatactcata	ctcttccttt	ttcaatatta	ttgaagcatt	5350
5351	tatcagggtt	attgtctcat	gagcggatac	atatttgaat	gtatttagaa	5400
5401	aaataaacaa	ataggggttc	cgcgcacatt	tccccgaaaa	gtgccacctg	5450
5451	aaattgtaaa	cgttaatatt	ttgttaaaat	tcgcgttaaa	tttttgttaa	5500
5501	atcagctcat	tttttaacca	ataggccgaa	atcggcaaaa	tcccttataa	5550
5551	atcaaaagaa	tagaccgaga	tagggttgag	tgttgttcca	gtttggaaca	5600
5601	agagtccact	attaaagaac	gtggactcca	acgtcaaagg	gcgaaaaacc	5650
5651	gtctatcagg	gcgatggccc	actacgtgaa	ccatcaccct	aatcaagttt	5700
5701	tttggggtcg	aggtgccgta	aagcactaaa	tcggaaccct	aaagggagcc	5750
5751	cccgatttag	agcttgacgg	ggaaagccgg	cgaacgtggc	gagaaaggaa	5800
5801	gggaagaaag	cgaaaggagc	gggcgctagg	gcgctggcaa	gtgtagcggt	5850
5851	cacgctgcgc	gtaaccacca	cacccgccgc	gcttaatgcg	ccgctacagg	5900
5901	gcgcgtccca	ttcgcca	5917