pET-30Ek/LIC

名称	编号	规格	价格	手册
pET-30Ek/LIC(pET30Ek/LIC)(60908-2795)	60908-2795

质粒编号: 60908-2795
质粒名称: pET-30Ek/LIC
其他名称:
质粒大小（bp）: 5439
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-His, N-S, N-EK, C-His
5′ 测序引物: T7

5′ 测序引物序列:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site

质粒图谱:

Feature Name	Start	End
T7_terminator	129	1
T7_Terminal_primer	69	87
EK	250	236
lacI	319	1925
T7_transl_en_RBS	389	373
lacO	434	407
T7_promoter	452	434
tet (300 – 563)	488	751
pBRrevBam_primer	559	540
tet (576 – 851)	1984	2259
ROP	2734	2925
pGEX_3_primer	2941	2919
pBR322_origin	3959	3340
KanR2	4065	4880

ORF	Start	End
ORF frame 2	344	1093
ORF frame 3	966	1925
ORF frame 3	4065	4880

Enzyme Name	Cut
XhoI	158
NotI	166
EagI	166
HindIII	173
SalI	179
SacI	190
EcoRI	192
BamHI	198
EcoRV	206
NcoI	211
KpnI	255
BglII	258
BstBI	285
NdeI	363
XbaI	401
BclI	1207
ApaI	1404
HpaI	1699
FspI	2275
NruI	4153

质粒序列:

1	atccggatat	agttcctcct	ttcagcaaaa	aacccctcaa	gacccgttta	50
51	gaggccccaa	ggggttatgc	tagttattgc	tcagcggtgg	cagcagccaa	100
101	ctcagcttcc	tttcgggctt	tgttagcagc	cggatctcag	tggtggtggt	150
151	ggtggtgctc	gagtgcggcc	gcaagcttgt	cgacggagct	cgaattcgga	200
201	tccgatatcg	ccatggttga	ggagaagccc	gggctcttgt	cgtcgtcatc	250
251	ggtacccaga	tctgggctgt	ccatgtgctg	gcgttcgaat	ttagcagcag	300
301	cggtttcttt	cataccagaa	ccgcgtggca	ccagaccaga	agaatgatga	350
351	tgatgatggt	gcatatgtat	atctccttct	taaagttaaa	caaaattatt	400
401	tctagagggg	aattgttatc	cgctcacaat	tcccctatag	tgagtcgtat	450
451	taatttcgcg	ggatcgagat	cgatctcgat	cctctacgcc	ggacgcatcg	500
501	tggccggcat	caccggcgcc	acaggtgcgg	ttgctggcgc	ctatatcgcc	550
551	gacatcaccg	atggggaaga	tcgggctcgc	cacttcgggc	tcatgagcgc	600
601	ttgtttcggc	gtgggtatgg	tggcaggccc	cgtggccggg	ggactgttgg	650
651	gcgccatctc	cttgcatgca	ccattccttg	cggcggcggt	gctcaacggc	700
701	ctcaacctac	tactgggctg	cttcctaatg	caggagtcgc	ataagggaga	750
751	gcgtcgagat	cccggacacc	atcgaatggc	gcaaaacctt	tcgcggtatg	800
801	gcatgatagc	gcccggaaga	gagtcaattc	agggtggtga	atgtgaaacc	850
851	agtaacgtta	tacgatgtcg	cagagtatgc	cggtgtctct	tatcagaccg	900
901	tttcccgcgt	ggtgaaccag	gccagccacg	tttctgcgaa	aacgcgggaa	950
951	aaagtggaag	cggcgatggc	ggagctgaat	tacattccca	accgcgtggc	1000
1001	acaacaactg	gcgggcaaac	agtcgttgct	gattggcgtt	gccacctcca	1050
1051	gtctggccct	gcacgcgccg	tcgcaaattg	tcgcggcgat	taaatctcgc	1100
1101	gccgatcaac	tgggtgccag	cgtggtggtg	tcgatggtag	aacgaagcgg	1150
1151	cgtcgaagcc	tgtaaagcgg	cggtgcacaa	tcttctcgcg	caacgcgtca	1200
1201	gtgggctgat	cattaactat	ccgctggatg	accaggatgc	cattgctgtg	1250
1251	gaagctgcct	gcactaatgt	tccggcgtta	tttcttgatg	tctctgacca	1300
1301	gacacccatc	aacagtatta	ttttctccca	tgaagacggt	acgcgactgg	1350
1351	gcgtggagca	tctggtcgca	ttgggtcacc	agcaaatcgc	gctgttagcg	1400
1401	ggcccattaa	gttctgtctc	ggcgcgtctg	cgtctggctg	gctggcataa	1450
1451	atatctcact	cgcaatcaaa	ttcagccgat	agcggaacgg	gaaggcgact	1500
1501	ggagtgccat	gtccggtttt	caacaaacca	tgcaaatgct	gaatgagggc	1550
1551	atcgttccca	ctgcgatgct	ggttgccaac	gatcagatgg	cgctgggcgc	1600
1601	aatgcgcgcc	attaccgagt	ccgggctgcg	cgttggtgcg	gacatctcgg	1650
1651	tagtgggata	cgacgatacc	gaagacagct	catgttatat	cccgccgtta	1700
1701	accaccatca	aacaggattt	tcgcctgctg	gggcaaacca	gcgtggaccg	1750
1751	cttgctgcaa	ctctctcagg	gccaggcggt	gaagggcaat	cagctgttgc	1800
1801	ccgtctcact	ggtgaaaaga	aaaaccaccc	tggcgcccaa	tacgcaaacc	1850
1851	gcctctcccc	gcgcgttggc	cgattcatta	atgcagctgg	cacgacaggt	1900
1901	ttcccgactg	gaaagcgggc	agtgagcgca	acgcaattaa	tgtaagttag	1950
1951	ctcactcatt	aggcaccggg	atctcgaccg	atgcccttga	gagccttcaa	2000
2001	cccagtcagc	tccttccggt	gggcgcgggg	catgactatc	gtcgccgcac	2050
2051	ttatgactgt	cttctttatc	atgcaactcg	taggacaggt	gccggcagcg	2100
2101	ctctgggtca	ttttcggcga	ggaccgcttt	cgctggagcg	cgacgatgat	2150
2151	cggcctgtcg	cttgcggtat	tcggaatctt	gcacgccctc	gctcaagcct	2200
2201	tcgtcactgg	tcccgccacc	aaacgtttcg	gcgagaagca	ggccattatc	2250
2251	gccggcatgg	cggccccacg	ggtgcgcatg	atcgtgctcc	tgtcgttgag	2300
2301	gacccggcta	ggctggcggg	gttgccttac	tggttagcag	aatgaatcac	2350
2351	cgatacgcga	gcgaacgtga	agcgactgct	gctgcaaaac	gtctgcgacc	2400
2401	tgagcaacaa	catgaatggt	cttcggtttc	cgtgtttcgt	aaagtctgga	2450
2451	aacgcggaag	tcagcgccct	gcaccattat	gttccggatc	tgcatcgcag	2500
2501	gatgctgctg	gctaccctgt	ggaacaccta	catctgtatt	aacgaagcgc	2550
2551	tggcattgac	cctgagtgat	ttttctctgg	tcccgccgca	tccataccgc	2600
2601	cagttgttta	ccctcacaac	gttccagtaa	ccgggcatgt	tcatcatcag	2650
2651	taacccgtat	cgtgagcatc	ctctctcgtt	tcatcggtat	cattaccccc	2700
2701	atgaacagaa	atccccctta	cacggaggca	tcagtgacca	aacaggaaaa	2750
2751	aaccgccctt	aacatggccc	gctttatcag	aagccagaca	ttaacgcttc	2800
2801	tggagaaact	caacgagctg	gacgcggatg	aacaggcaga	catctgtgaa	2850
2851	tcgcttcacg	accacgctga	tgagctttac	cgcagctgcc	tcgcgcgttt	2900
2901	cggtgatgac	ggtgaaaacc	tctgacacat	gcagctcccg	gagacggtca	2950
2951	cagcttgtct	gtaagcggat	gccgggagca	gacaagcccg	tcagggcgcg	3000
3001	tcagcgggtg	ttggcgggtg	tcggggcgca	gccatgaccc	agtcacgtag	3050
3051	cgatagcgga	gtgtatactg	gcttaactat	gcggcatcag	agcagattgt	3100
3101	actgagagtg	caccatatat	gcggtgtgaa	ataccgcaca	gatgcgtaag	3150
3151	gagaaaatac	cgcatcaggc	gctcttccgc	ttcctcgctc	actgactcgc	3200
3201	tgcgctcggt	cgttcggctg	cggcgagcgg	tatcagctca	ctcaaaggcg	3250
3251	gtaatacggt	tatccacaga	atcaggggat	aacgcaggaa	agaacatgtg	3300
3301	agcaaaaggc	cagcaaaagg	ccaggaaccg	taaaaaggcc	gcgttgctgg	3350
3351	cgtttttcca	taggctccgc	ccccctgacg	agcatcacaa	aaatcgacgc	3400
3401	tcaagtcaga	ggtggcgaaa	cccgacagga	ctataaagat	accaggcgtt	3450
3451	tccccctgga	agctccctcg	tgcgctctcc	tgttccgacc	ctgccgctta	3500
3501	ccggatacct	gtccgccttt	ctcccttcgg	gaagcgtggc	gctttctcat	3550
3551	agctcacgct	gtaggtatct	cagttcggtg	taggtcgttc	gctccaagct	3600
3601	gggctgtgtg	cacgaacccc	ccgttcagcc	cgaccgctgc	gccttatccg	3650
3651	gtaactatcg	tcttgagtcc	aacccggtaa	gacacgactt	atcgccactg	3700
3701	gcagcagcca	ctggtaacag	gattagcaga	gcgaggtatg	taggcggtgc	3750
3751	tacagagttc	ttgaagtggt	ggcctaacta	cggctacact	agaaggacag	3800
3801	tatttggtat	ctgcgctctg	ctgaagccag	ttaccttcgg	aaaaagagtt	3850
3851	ggtagctctt	gatccggcaa	acaaaccacc	gctggtagcg	gtggtttttt	3900
3901	tgtttgcaag	cagcagatta	cgcgcagaaa	aaaaggatct	caagaagatc	3950
3951	ctttgatctt	ttctacgggg	tctgacgctc	agtggaacga	aaactcacgt	4000
4001	taagggattt	tggtcatgaa	caataaaact	gtctgcttac	ataaacagta	4050
4051	atacaagggg	tgttatgagc	catattcaac	gggaaacgtc	ttgctctagg	4100
4101	ccgcgattaa	attccaacat	ggatgctgat	ttatatgggt	ataaatgggc	4150
4151	tcgcgataat	gtcgggcaat	caggtgcgac	aatctatcga	ttgtatggga	4200
4201	agcccgatgc	gccagagttg	tttctgaaac	atggcaaagg	tagcgttgcc	4250
4251	aatgatgtta	cagatgagat	ggtcagacta	aactggctga	cggaatttat	4300
4301	gcctcttccg	accatcaagc	attttatccg	tactcctgat	gatgcatggt	4350
4351	tactcaccac	tgcgatcccc	gggaaaacag	cattccaggt	attagaagaa	4400
4401	tatcctgatt	caggtgaaaa	tattgttgat	gcgctggcag	tgttcctgcg	4450
4451	ccggttgcat	tcgattcctg	tttgtaattg	tccttttaac	agcgatcgcg	4500
4501	tatttcgtct	cgctcaggcg	caatcacgaa	tgaataacgg	tttggttgat	4550
4551	gcgagtgatt	ttgatgacga	gcgtaatggc	tggcctgttg	aacaagtctg	4600
4601	gaaagaaatg	cataaacttt	tgccattctc	accggattca	gtcgtcactc	4650
4651	atggtgattt	ctcacttgat	aaccttattt	ttgacgaggg	gaaattaata	4700
4701	ggttgtattg	atgttggacg	agtcggaatc	gcagaccgat	accaggatct	4750
4751	tgccatccta	tggaactgcc	tcggtgagtt	ttctccttca	ttacagaaac	4800
4801	ggctttttca	aaaatatggt	attgataatc	ctgatatgaa	taaattgcag	4850
4851	tttcatttga	tgctcgatga	gtttttctaa	gaattaattc	atgagcggat	4900
4901	acatatttga	atgtatttag	aaaaataaac	aaataggggt	tccgcgcaca	4950
4951	tttccccgaa	aagtgccacc	tgaaattgta	aacgttaata	ttttgttaaa	5000
5001	attcgcgtta	aatttttgtt	aaatcagctc	attttttaac	caataggccg	5050
5051	aaatcggcaa	aatcccttat	aaatcaaaag	aatagaccga	gatagggttg	5100
5101	agtgttgttc	cagtttggaa	caagagtcca	ctattaaaga	acgtggactc	5150
5151	caacgtcaaa	gggcgaaaaa	ccgtctatca	gggcgatggc	ccactacgtg	5200
5201	aaccatcacc	ctaatcaagt	tttttggggt	cgaggtgccg	taaagcacta	5250
5251	aatcggaacc	ctaaagggag	cccccgattt	agagcttgac	ggggaaagcc	5300
5301	ggcgaacgtg	gcgagaaagg	aagggaagaa	agcgaaagga	gcgggcgcta	5350
5351	gggcgctggc	aagtgtagcg	gtcacgctgc	gcgtaaccac	cacacccgcc	5400
5401	gcgcttaatg	cgccgctaca	gggcgcgtcc	cattcgcca	5439