pET-46Ek/LIC

名称	编号	规格	价格	手册
pET-46Ek/LIC(pET46Ek/LIC)（60908-2814）	60908-2814

质粒编号: 60908-2814
质粒名称: pET-46Ek/LIC
其他名称:
质粒大小（bp）: 5200
抗性:Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: 高
蛋白标签: N-His, N-EK, C-S
5′ 测序引物序列: T7: 5′-TAATACGACTCACTATAGGG-3′

3′ 测序引物序列: T7t: 5′-TGCTAGTTATTGCTCAGCGG-3′
GenBank号:
其他属性: For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site.
质粒图谱:

Feature Name	Start	End
Ampicillin	1442	582
AmpR_promoter	1513	1485
pBR322_origin	1600	2219
pGEX_3_primer	2618	2640
ROP	2825	2634
lacI	4488	3397
tet (887 – 636)	4831	4580
pBRrevBam_primer	4763	4782
T7_promoter	4870	4888
lacO	4888	4915
T7_transl_en_RBS	4933	4949
EK	4984	4998
T7_Terminal_primer	5132	5114
T7_terminator	5133	5168

ORF	Start	End
ORF frame 3	1442	582
ORF frame 2	4356	3397
ORF frame 1	4729	4229

Enzyme Name	Cut
FspI	877
HpaI	3622
ApaI	3921
BclI	4110
ClaI	4849
XbaI	4916
NcoI	4955
PmlI	4980
XhoI	5020
PacI	5095

质粒序列:

1	tggcgaatgg	gacgcgccct	gtagcggcgc	attaagcgcg	gcgggtgtgg	50
51	tggttacgcg	cagcgtgacc	gctacacttg	ccagcgccct	agcgcccgct	100
101	cctttcgctt	tcttcccttc	ctttctcgcc	acgttcgccg	gctttccccg	150
151	tcaagctcta	aatcgggggc	tccctttagg	gttccgattt	agtgctttac	200
201	ggcacctcga	ccccaaaaaa	cttgattagg	gtgatggttc	acgtagtggg	250
251	ccatcgccct	gatagacggt	ttttcgccct	ttgacgttgg	agtccacgtt	300
301	ctttaatagt	ggactcttgt	tccaaactgg	aacaacactc	aaccctatct	350
351	cggtctattc	ttttgattta	taagggattt	tgccgatttc	ggcctattgg	400
401	ttaaaaaatg	agctgattta	acaaaaattt	aacgcgaatt	ttaacaaaat	450
451	attaacgttt	acaatttctg	gcggcacgat	ggcatgagat	tatcaaaaag	500
501	gatcttcacc	tagatccttt	taaattaaaa	atgaagtttt	aaatcaatct	550
551	aaagtatata	tgagtaaact	tggtctgaca	gttaccaatg	cttaatcagt	600
601	gaggcaccta	tctcagcgat	ctgtctattt	cgttcatcca	tagttgcctg	650
651	actccccgtc	gtgtagataa	ctacgatacg	ggagggctta	ccatctggcc	700
701	ccagtgctgc	aatgataccg	cgagacccac	gctcaccggc	tccagattta	750
751	tcagcaataa	accagccagc	cggaagggcc	gagcgcagaa	gtggtcctgc	800
801	aactttatcc	gcctccatcc	agtctattaa	ttgttgccgg	gaagctagag	850
851	taagtagttc	gccagttaat	agtttgcgca	acgttgttgc	cattgctaca	900
901	ggcatcgtgg	tgtcacgctc	gtcgtttggt	atggcttcat	tcagctccgg	950
951	ttcccaacga	tcaaggcgag	ttacatgatc	ccccatgttg	tgcaaaaaag	1000
1001	cggttagctc	cttcggtcct	ccgatcgttg	tcagaagtaa	gttggccgca	1050
1051	gtgttatcac	tcatggttat	ggcagcactg	cataattctc	ttactgtcat	1100
1101	gccatccgta	agatgctttt	ctgtgactgg	tgagtactca	accaagtcat	1150
1151	tctgagaata	gtgtatgcgg	cgaccgagtt	gctcttgccc	ggcgtcaata	1200
1201	cgggataata	ccgcgccaca	tagcagaact	ttaaaagtgc	tcatcattgg	1250
1251	aaaacgttct	tcggggcgaa	aactctcaag	gatcttaccg	ctgttgagat	1300
1301	ccagttcgat	gtaacccact	cgtgcaccca	actgatcttc	agcatctttt	1350
1351	actttcacca	gcgtttctgg	gtgagcaaaa	acaggaaggc	aaaatgccgc	1400
1401	aaaaaaggga	ataagggcga	cacggaaatg	ttgaatactc	atactcttcc	1450
1451	tttttcaatc	atgattgaag	catttatcag	ggttattgtc	tcatgagcgg	1500
1501	atacatattt	gaatgtattt	agaaaaataa	acaaataggt	catgaccaaa	1550
1551	atcccttaac	gtgagttttc	gttccactga	gcgtcagacc	ccgtagaaaa	1600
1601	gatcaaagga	tcttcttgag	atcctttttt	tctgcgcgta	atctgctgct	1650
1651	tgcaaacaaa	aaaaccaccg	ctaccagcgg	tggtttgttt	gccggatcaa	1700
1701	gagctaccaa	ctctttttcc	gaaggtaact	ggcttcagca	gagcgcagat	1750
1751	accaaatact	gtccttctag	tgtagccgta	gttaggccac	cacttcaaga	1800
1801	actctgtagc	accgcctaca	tacctcgctc	tgctaatcct	gttaccagtg	1850
1851	gctgctgcca	gtggcgataa	gtcgtgtctt	accgggttgg	actcaagacg	1900
1901	atagttaccg	gataaggcgc	agcggtcggg	ctgaacgggg	ggttcgtgca	1950
1951	cacagcccag	cttggagcga	acgacctaca	ccgaactgag	atacctacag	2000
2001	cgtgagctat	gagaaagcgc	cacgcttccc	gaagggagaa	aggcggacag	2050
2051	gtatccggta	agcggcaggg	tcggaacagg	agagcgcacg	agggagcttc	2100
2101	cagggggaaa	cgcctggtat	ctttatagtc	ctgtcgggtt	tcgccacctc	2150
2151	tgacttgagc	gtcgattttt	gtgatgctcg	tcaggggggc	ggagcctatg	2200
2201	gaaaaacgcc	agcaacgcgg	cctttttacg	gttcctggcc	ttttgctggc	2250
2251	cttttgctca	catgttcttt	cctgcgttat	cccctgattc	tgtggataac	2300
2301	cgtattaccg	cctttgagtg	agctgatacc	gctcgccgca	gccgaacgac	2350
2351	cgagcgcagc	gagtcagtga	gcgaggaagc	ggaagagcgc	ctgatgcggt	2400
2401	attttctcct	tacgcatctg	tgcggtattt	cacaccgcat	atatggtgca	2450
2451	ctctcagtac	aatctgctct	gatgccgcat	agttaagcca	gtatacactc	2500
2501	cgctatcgct	acgtgactgg	gtcatggctg	cgccccgaca	cccgccaaca	2550
2551	cccgctgacg	cgccctgacg	ggcttgtctg	ctcccggcat	ccgcttacag	2600
2601	acaagctgtg	accgtctccg	ggagctgcat	gtgtcagagg	ttttcaccgt	2650
2651	catcaccgaa	acgcgcgagg	cagctgcggt	aaagctcatc	agcgtggtcg	2700
2701	tgaagcgatt	cacagatgtc	tgcctgttca	tccgcgtcca	gctcgttgag	2750
2751	tttctccaga	agcgttaatg	tctggcttct	gataaagcgg	gccatgttaa	2800
2801	gggcggtttt	ttcctgtttg	gtcactgatg	cctccgtgta	agggggattt	2850
2851	ctgttcatgg	gggtaatgat	accgatgaaa	cgagagagga	tgctcacgat	2900
2901	acgggttact	gatgatgaac	atgcccggtt	actggaacgt	tgtgagggta	2950
2951	aacaactggc	ggtatggatg	cggcgggacc	agagaaaaat	cactcagggt	3000
3001	caatgccagc	gcttcgttaa	tacagatgta	ggtgttccac	agggtagcca	3050
3051	gcagcatcct	gcgatgcaga	tccggaacat	aatggtgcag	ggcgctgact	3100
3101	tccgcgtttc	cagactttac	gaaacacgga	aaccgaagac	cattcatgtt	3150
3151	gttgctcagg	tcgcagacgt	tttgcagcag	cagtcgcttc	acgttcgctc	3200
3201	gcgtatcggt	gattcattct	gctaaccagt	aaggcaaccc	cgccagccta	3250
3251	gccgggtcct	caacgacagg	agcacgatca	tgctagtcat	gccccgcgcc	3300
3301	caccggaagg	agctgactgg	gttgaaggct	ctcaagggca	tcggtcgaga	3350
3351	tcccggtgcc	taatgagtga	gctaacttac	attaattgcg	ttgcgctcac	3400
3401	tgcccgcttt	ccagtcggga	aacctgtcgt	gccagctgca	ttaatgaatc	3450
3451	ggccaacgcg	cggggagagg	cggtttgcgt	attgggcgcc	agggtggttt	3500
3501	ttcttttcac	cagtgagacg	ggcaacagct	gattgccctt	caccgcctgg	3550
3551	ccctgagaga	gttgcagcaa	gcggtccacg	ctggtttgcc	ccagcaggcg	3600
3601	aaaatcctgt	ttgatggtgg	ttaacggcgg	gatataacat	gagctgtctt	3650
3651	cggtatcgtc	gtatcccact	accgagatgt	ccgcaccaac	gcgcagcccg	3700
3701	gactcggtaa	tggcgcgcat	tgcgcccagc	gccatctgat	cgttggcaac	3750
3751	cagcatcgca	gtgggaacga	tgccctcatt	cagcatttgc	atggtttgtt	3800
3801	gaaaaccgga	catggcactc	cagtcgcctt	cccgttccgc	tatcggctga	3850
3851	atttgattgc	gagtgagata	tttatgccag	ccagccagac	gcagacgcgc	3900
3901	cgagacagaa	cttaatgggc	ccgctaacag	cgcgatttgc	tggtgaccca	3950
3951	atgcgaccag	atgctccacg	cccagtcgcg	taccgtcttc	atgggagaaa	4000
4001	ataatactgt	tgatgggtgt	ctggtcagag	acatcaagaa	ataacgccgg	4050
4051	aacattagtg	caggcagctt	ccacagcaat	ggcatcctgg	tcatccagcg	4100
4101	gatagttaat	gatcagccca	ctgacgcgtt	gcgcgagaag	attgtgcacc	4150
4151	gccgctttac	aggcttcgac	gccgcttcgt	tctaccatcg	acaccaccac	4200
4201	gctggcaccc	agttgatcgg	cgcgagattt	aatcgccgcg	acaatttgcg	4250
4251	acggcgcgtg	cagggccaga	ctggaggtgg	caacgccaat	cagcaacgac	4300
4301	tgtttgcccg	ccagttgttg	tgccacgcgg	ttgggaatgt	aattcagctc	4350
4351	cgccatcgcc	gcttccactt	tttcccgcgt	tttcgcagaa	acgtggctgg	4400
4401	cctggttcac	cacgcgggaa	acggtctgat	aagagacacc	ggcatactct	4450
4451	gcgacatcgt	ataacgttac	tggtttcaca	ttcaccaccc	tgaattgact	4500
4501	ctcttccggg	cgctatcatg	ccataccgcg	aaaggttttg	cgccattcga	4550
4551	tggtgtccgg	gatctcgacg	ctctccctta	tgcgactcct	gcattaggaa	4600
4601	gcagcccagt	agtaggttga	ggccgttgag	caccgccgcc	gcaaggaatg	4650
4651	gtgcatgcaa	ggagatggcg	cccaacagtc	ccccggccac	ggggcctgcc	4700
4701	accataccca	cgccgaaaca	agcgctcatg	agcccgaagt	ggcgagcccg	4750
4751	atcttcccca	tcggtgatgt	cggcgatata	ggcgccagca	accgcacctg	4800
4801	tggcgccggt	gatgccggcc	acgatgcgtc	cggcgtagag	gatcgagatc	4850
4851	gatctcgatc	ccgcgaaatt	aatacgactc	actatagggg	aattgtgagc	4900
4901	ggataacaat	tcccctctag	aaataatttt	gtttaacttt	aagaaggaga	4950
4951	tataccatgg	cacatcacca	ccaccatcac	gtggatgacg	acgacaagag	5000
5001	tccgggcttc	tcctcaaatc	tcgagtctgg	taaagaaacc	gctgctgcga	5050
5051	aatttgaacg	ccagcacatg	gactcgtcta	ctagcgcagc	ttaattaacc	5100
5101	taggctgctg	ccaccgctga	gcaataacta	gcataacccc	ttggggcctc	5150
5151	taaacgggtc	ttgaggggtt	ttttgctgaa	aggaggaact	atatccggat	5200