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- ZK-0023694
- 中国
- 2025年11月21日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-30Ek/LIC
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2795
质粒名称: pET-30Ek/LIC
其他名称:
质粒大小（bp）: 5439
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-His, N-S, N-EK, C-His
5′ 测序引物: T7
5′ 测序引物序列:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| EK | 250 | 236 | |
| lacI | 319 | 1925 | |
| T7_transl_en_RBS | 389 | 373 | |
| lacO | 434 | 407 | |
| T7_promoter | 452 | 434 | |
| tet (300 – 563) | 488 | 751 | |
| pBRrevBam_primer | 559 | 540 | |
| tet (576 – 851) | 1984 | 2259 | |
| ROP | 2734 | 2925 | |
| pGEX_3_primer | 2941 | 2919 | |
| pBR322_origin | 3959 | 3340 | |
| KanR2 | 4065 | 4880 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 344 | 1093 | |
| ORF frame 3 | 966 | 1925 | |
| ORF frame 3 | 4065 | 4880 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| EcoRV | 206 |
| NcoI | 211 |
| KpnI | 255 |
| BglII | 258 |
| BstBI | 285 |
| NdeI | 363 |
| XbaI | 401 |
| BclI | 1207 |
| ApaI | 1404 |
| HpaI | 1699 |
| FspI | 2275 |
| NruI | 4153 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccgatatcg | ccatggttga | ggagaagccc | gggctcttgt | cgtcgtcatc | 250 |
| 251 | ggtacccaga | tctgggctgt | ccatgtgctg | gcgttcgaat | ttagcagcag | 300 |
| 301 | cggtttcttt | cataccagaa | ccgcgtggca | ccagaccaga | agaatgatga | 350 |
| 351 | tgatgatggt | gcatatgtat | atctccttct | taaagttaaa | caaaattatt | 400 |
| 401 | tctagagggg | aattgttatc | cgctcacaat | tcccctatag | tgagtcgtat | 450 |
| 451 | taatttcgcg | ggatcgagat | cgatctcgat | cctctacgcc | ggacgcatcg | 500 |
| 501 | tggccggcat | caccggcgcc | acaggtgcgg | ttgctggcgc | ctatatcgcc | 550 |
| 551 | gacatcaccg | atggggaaga | tcgggctcgc | cacttcgggc | tcatgagcgc | 600 |
| 601 | ttgtttcggc | gtgggtatgg | tggcaggccc | cgtggccggg | ggactgttgg | 650 |
| 651 | gcgccatctc | cttgcatgca | ccattccttg | cggcggcggt | gctcaacggc | 700 |
| 701 | ctcaacctac | tactgggctg | cttcctaatg | caggagtcgc | ataagggaga | 750 |
| 751 | gcgtcgagat | cccggacacc | atcgaatggc | gcaaaacctt | tcgcggtatg | 800 |
| 801 | gcatgatagc | gcccggaaga | gagtcaattc | agggtggtga | atgtgaaacc | 850 |
| 851 | agtaacgtta | tacgatgtcg | cagagtatgc | cggtgtctct | tatcagaccg | 900 |
| 901 | tttcccgcgt | ggtgaaccag | gccagccacg | tttctgcgaa | aacgcgggaa | 950 |
| 951 | aaagtggaag | cggcgatggc | ggagctgaat | tacattccca | accgcgtggc | 1000 |
| 1001 | acaacaactg | gcgggcaaac | agtcgttgct | gattggcgtt | gccacctcca | 1050 |
| 1051 | gtctggccct | gcacgcgccg | tcgcaaattg | tcgcggcgat | taaatctcgc | 1100 |
| 1101 | gccgatcaac | tgggtgccag | cgtggtggtg | tcgatggtag | aacgaagcgg | 1150 |
| 1151 | cgtcgaagcc | tgtaaagcgg | cggtgcacaa | tcttctcgcg | caacgcgtca | 1200 |
| 1201 | gtgggctgat | cattaactat | ccgctggatg | accaggatgc | cattgctgtg | 1250 |
| 1251 | gaagctgcct | gcactaatgt | tccggcgtta | tttcttgatg | tctctgacca | 1300 |
| 1301 | gacacccatc | aacagtatta | ttttctccca | tgaagacggt | acgcgactgg | 1350 |
| 1351 | gcgtggagca | tctggtcgca | ttgggtcacc | agcaaatcgc | gctgttagcg | 1400 |
| 1401 | ggcccattaa | gttctgtctc | ggcgcgtctg | cgtctggctg | gctggcataa | 1450 |
| 1451 | atatctcact | cgcaatcaaa | ttcagccgat | agcggaacgg | gaaggcgact | 1500 |
| 1501 | ggagtgccat | gtccggtttt | caacaaacca | tgcaaatgct | gaatgagggc | 1550 |
| 1551 | atcgttccca | ctgcgatgct | ggttgccaac | gatcagatgg | cgctgggcgc | 1600 |
| 1601 | aatgcgcgcc | attaccgagt | ccgggctgcg | cgttggtgcg | gacatctcgg | 1650 |
| 1651 | tagtgggata | cgacgatacc | gaagacagct | catgttatat | cccgccgtta | 1700 |
| 1701 | accaccatca | aacaggattt | tcgcctgctg | gggcaaacca | gcgtggaccg | 1750 |
| 1751 | cttgctgcaa | ctctctcagg | gccaggcggt | gaagggcaat | cagctgttgc | 1800 |
| 1801 | ccgtctcact | ggtgaaaaga | aaaaccaccc | tggcgcccaa | tacgcaaacc | 1850 |
| 1851 | gcctctcccc | gcgcgttggc | cgattcatta | atgcagctgg | cacgacaggt | 1900 |
| 1901 | ttcccgactg | gaaagcgggc | agtgagcgca | acgcaattaa | tgtaagttag | 1950 |
| 1951 | ctcactcatt | aggcaccggg | atctcgaccg | atgcccttga | gagccttcaa | 2000 |
| 2001 | cccagtcagc | tccttccggt | gggcgcgggg | catgactatc | gtcgccgcac | 2050 |
| 2051 | ttatgactgt | cttctttatc | atgcaactcg | taggacaggt | gccggcagcg | 2100 |
| 2101 | ctctgggtca | ttttcggcga | ggaccgcttt | cgctggagcg | cgacgatgat | 2150 |
| 2151 | cggcctgtcg | cttgcggtat | tcggaatctt | gcacgccctc | gctcaagcct | 2200 |
| 2201 | tcgtcactgg | tcccgccacc | aaacgtttcg | gcgagaagca | ggccattatc | 2250 |
| 2251 | gccggcatgg | cggccccacg | ggtgcgcatg | atcgtgctcc | tgtcgttgag | 2300 |
| 2301 | gacccggcta | ggctggcggg | gttgccttac | tggttagcag | aatgaatcac | 2350 |
| 2351 | cgatacgcga | gcgaacgtga | agcgactgct | gctgcaaaac | gtctgcgacc | 2400 |
| 2401 | tgagcaacaa | catgaatggt | cttcggtttc | cgtgtttcgt | aaagtctgga | 2450 |
| 2451 | aacgcggaag | tcagcgccct | gcaccattat | gttccggatc | tgcatcgcag | 2500 |
| 2501 | gatgctgctg | gctaccctgt | ggaacaccta | catctgtatt | aacgaagcgc | 2550 |
| 2551 | tggcattgac | cctgagtgat | ttttctctgg | tcccgccgca | tccataccgc | 2600 |
| 2601 | cagttgttta | ccctcacaac | gttccagtaa | ccgggcatgt | tcatcatcag | 2650 |
| 2651 | taacccgtat | cgtgagcatc | ctctctcgtt | tcatcggtat | cattaccccc | 2700 |
| 2701 | atgaacagaa | atccccctta | cacggaggca | tcagtgacca | aacaggaaaa | 2750 |
| 2751 | aaccgccctt | aacatggccc | gctttatcag | aagccagaca | ttaacgcttc | 2800 |
| 2801 | tggagaaact | caacgagctg | gacgcggatg | aacaggcaga | catctgtgaa | 2850 |
| 2851 | tcgcttcacg | accacgctga | tgagctttac | cgcagctgcc | tcgcgcgttt | 2900 |
| 2901 | cggtgatgac | ggtgaaaacc | tctgacacat | gcagctcccg | gagacggtca | 2950 |
| 2951 | cagcttgtct | gtaagcggat | gccgggagca | gacaagcccg | tcagggcgcg | 3000 |
| 3001 | tcagcgggtg | ttggcgggtg | tcggggcgca | gccatgaccc | agtcacgtag | 3050 |
| 3051 | cgatagcgga | gtgtatactg | gcttaactat | gcggcatcag | agcagattgt | 3100 |
| 3101 | actgagagtg | caccatatat | gcggtgtgaa | ataccgcaca | gatgcgtaag | 3150 |
| 3151 | gagaaaatac | cgcatcaggc | gctcttccgc | ttcctcgctc | actgactcgc | 3200 |
| 3201 | tgcgctcggt | cgttcggctg | cggcgagcgg | tatcagctca | ctcaaaggcg | 3250 |
| 3251 | gtaatacggt | tatccacaga | atcaggggat | aacgcaggaa | agaacatgtg | 3300 |
| 3301 | agcaaaaggc | cagcaaaagg | ccaggaaccg | taaaaaggcc | gcgttgctgg | 3350 |
| 3351 | cgtttttcca | taggctccgc | ccccctgacg | agcatcacaa | aaatcgacgc | 3400 |
| 3401 | tcaagtcaga | ggtggcgaaa | cccgacagga | ctataaagat | accaggcgtt | 3450 |
| 3451 | tccccctgga | agctccctcg | tgcgctctcc | tgttccgacc | ctgccgctta | 3500 |
| 3501 | ccggatacct | gtccgccttt | ctcccttcgg | gaagcgtggc | gctttctcat | 3550 |
| 3551 | agctcacgct | gtaggtatct | cagttcggtg | taggtcgttc | gctccaagct | 3600 |
| 3601 | gggctgtgtg | cacgaacccc | ccgttcagcc | cgaccgctgc | gccttatccg | 3650 |
| 3651 | gtaactatcg | tcttgagtcc | aacccggtaa | gacacgactt | atcgccactg | 3700 |
| 3701 | gcagcagcca | ctggtaacag | gattagcaga | gcgaggtatg | taggcggtgc | 3750 |
| 3751 | tacagagttc | ttgaagtggt | ggcctaacta | cggctacact | agaaggacag | 3800 |
| 3801 | tatttggtat | ctgcgctctg | ctgaagccag | ttaccttcgg | aaaaagagtt | 3850 |
| 3851 | ggtagctctt | gatccggcaa | acaaaccacc | gctggtagcg | gtggtttttt | 3900 |
| 3901 | tgtttgcaag | cagcagatta | cgcgcagaaa | aaaaggatct | caagaagatc | 3950 |
| 3951 | ctttgatctt | ttctacgggg | tctgacgctc | agtggaacga | aaactcacgt | 4000 |
| 4001 | taagggattt | tggtcatgaa | caataaaact | gtctgcttac | ataaacagta | 4050 |
| 4051 | atacaagggg | tgttatgagc | catattcaac | gggaaacgtc | ttgctctagg | 4100 |
| 4101 | ccgcgattaa | attccaacat | ggatgctgat | ttatatgggt | ataaatgggc | 4150 |
| 4151 | tcgcgataat | gtcgggcaat | caggtgcgac | aatctatcga | ttgtatggga | 4200 |
| 4201 | agcccgatgc | gccagagttg | tttctgaaac | atggcaaagg | tagcgttgcc | 4250 |
| 4251 | aatgatgtta | cagatgagat | ggtcagacta | aactggctga | cggaatttat | 4300 |
| 4301 | gcctcttccg | accatcaagc | attttatccg | tactcctgat | gatgcatggt | 4350 |
| 4351 | tactcaccac | tgcgatcccc | gggaaaacag | cattccaggt | attagaagaa | 4400 |
| 4401 | tatcctgatt | caggtgaaaa | tattgttgat | gcgctggcag | tgttcctgcg | 4450 |
| 4451 | ccggttgcat | tcgattcctg | tttgtaattg | tccttttaac | agcgatcgcg | 4500 |
| 4501 | tatttcgtct | cgctcaggcg | caatcacgaa | tgaataacgg | tttggttgat | 4550 |
| 4551 | gcgagtgatt | ttgatgacga | gcgtaatggc | tggcctgttg | aacaagtctg | 4600 |
| 4601 | gaaagaaatg | cataaacttt | tgccattctc | accggattca | gtcgtcactc | 4650 |
| 4651 | atggtgattt | ctcacttgat | aaccttattt | ttgacgaggg | gaaattaata | 4700 |
| 4701 | ggttgtattg | atgttggacg | agtcggaatc | gcagaccgat | accaggatct | 4750 |
| 4751 | tgccatccta | tggaactgcc | tcggtgagtt | ttctccttca | ttacagaaac | 4800 |
| 4801 | ggctttttca | aaaatatggt | attgataatc | ctgatatgaa | taaattgcag | 4850 |
| 4851 | tttcatttga | tgctcgatga | gtttttctaa | gaattaattc | atgagcggat | 4900 |
| 4901 | acatatttga | atgtatttag | aaaaataaac | aaataggggt | tccgcgcaca | 4950 |
| 4951 | tttccccgaa | aagtgccacc | tgaaattgta | aacgttaata | ttttgttaaa | 5000 |
| 5001 | attcgcgtta | aatttttgtt | aaatcagctc | attttttaac | caataggccg | 5050 |
| 5051 | aaatcggcaa | aatcccttat | aaatcaaaag | aatagaccga | gatagggttg | 5100 |
| 5101 | agtgttgttc | cagtttggaa | caagagtcca | ctattaaaga | acgtggactc | 5150 |
| 5151 | caacgtcaaa | gggcgaaaaa | ccgtctatca | gggcgatggc | ccactacgtg | 5200 |
| 5201 | aaccatcacc | ctaatcaagt | tttttggggt | cgaggtgccg | taaagcacta | 5250 |
| 5251 | aatcggaacc | ctaaagggag | cccccgattt | agagcttgac | ggggaaagcc | 5300 |
| 5301 | ggcgaacgtg | gcgagaaagg | aagggaagaa | agcgaaagga | gcgggcgcta | 5350 |
| 5351 | gggcgctggc | aagtgtagcg | gtcacgctgc | gcgtaaccac | cacacccgcc | 5400 |
| 5401 | gcgcttaatg | cgccgctaca | gggcgcgtcc | cattcgcca | 5439 |
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pET-30Ek/LIC
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