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低温运输,-20℃保存
1年
pET-28c(+)
大量
信裕生物
名称 | 编号 | 规格 | 价格 | 手册 |
---|---|---|---|---|
pET-28c(+)(pET28c) | 60908-3425y |
质粒编号:60908-3425
质粒名称: pET-28c(+)
其他名称: pET28c, pet 28c
质粒大小(bp): 5367
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-His, N-Thrombin, N-T7, C-His
5’测序引物:T7
5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Nterm thrombin cleavage site; a,b,c vary by MCS;
质粒图谱:
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
T7_leader | 237 | 205 | |
Xpress_fwd_primer | 238 | 220 | |
T7_transl_en_RBS | 321 | 305 | |
lacO | 366 | 339 | |
T7_promoter | 384 | 366 | |
tet (300 – 563) | 416 | 679 | |
pBRrevBam_primer | 487 | 468 | |
lacI | 762 | 1853 | |
tet (576 – 851) | 1912 | 2187 | |
ROP | 2662 | 2853 | |
pGEX_3_primer | 2869 | 2847 | |
pBR322_origin | 3887 | 3268 | |
KanR2 | 3993 | 4808 |
ORF | Start | End | |
---|---|---|---|
ORF frame 2 | 521 | 1021 | |
ORF frame 3 | 894 | 1853 | |
ORF frame 3 | 3993 | 4808 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
NheI | 229 |
NdeI | 236 |
NcoI | 294 |
XbaI | 333 |
BglII | 399 |
BclI | 1135 |
ApaI | 1332 |
EcoRV | 1571 |
HpaI | 1627 |
FspI | 2203 |
NruI | 4081 |
ClaI | 4115 |
XmaI | 4296 |
SmaI | 4298 |
质粒序列:
1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
201 | tccgacccat | ttgctgtcca | ccagtcatgc | tagccatatg | gctgccgcgc | 250 |
251 | ggcaccaggc | cgctgctgtg | atgatgatga | tgatggctgc | tgcccatggt | 300 |
301 | atatctcctt | cttaaagtta | aacaaaatta | tttctagagg | ggaattgtta | 350 |
351 | tccgctcaca | attcccctat | agtgagtcgt | attaatttcg | cgggatcgag | 400 |
401 | atctcgatcc | tctacgccgg | acgcatcgtg | gccggcatca | ccggcgccac | 450 |
451 | aggtgcggtt | gctggcgcct | atatcgccga | catcaccgat | ggggaagatc | 500 |
501 | gggctcgcca | cttcgggctc | atgagcgctt | gtttcggcgt | gggtatggtg | 550 |
551 | gcaggccccg | tggccggggg | actgttgggc | gccatctcct | tgcatgcacc | 600 |
601 | attccttgcg | gcggcggtgc | tcaacggcct | caacctacta | ctgggctgct | 650 |
651 | tcctaatgca | ggagtcgcat | aagggagagc | gtcgagatcc | cggacaccat | 700 |
701 | cgaatggcgc | aaaacctttc | gcggtatggc | atgatagcgc | ccggaagaga | 750 |
751 | gtcaattcag | ggtggtgaat | gtgaaaccag | taacgttata | cgatgtcgca | 800 |
801 | gagtatgccg | gtgtctctta | tcagaccgtt | tcccgcgtgg | tgaaccaggc | 850 |
851 | cagccacgtt | tctgcgaaaa | cgcgggaaaa | agtggaagcg | gcgatggcgg | 900 |
901 | agctgaatta | cattcccaac | cgcgtggcac | aacaactggc | gggcaaacag | 950 |
951 | tcgttgctga | ttggcgttgc | cacctccagt | ctggccctgc | acgcgccgtc | 1000 |
1001 | gcaaattgtc | gcggcgatta | aatctcgcgc | cgatcaactg | ggtgccagcg | 1050 |
1051 | tggtggtgtc | gatggtagaa | cgaagcggcg | tcgaagcctg | taaagcggcg | 1100 |
1101 | gtgcacaatc | ttctcgcgca | acgcgtcagt | gggctgatca | ttaactatcc | 1150 |
1151 | gctggatgac | caggatgcca | ttgctgtgga | agctgcctgc | actaatgttc | 1200 |
1201 | cggcgttatt | tcttgatgtc | tctgaccaga | cacccatcaa | cagtattatt | 1250 |
1251 | ttctcccatg | aagacggtac | gcgactgggc | gtggagcatc | tggtcgcatt | 1300 |
1301 | gggtcaccag | caaatcgcgc | tgttagcggg | cccattaagt | tctgtctcgg | 1350 |
1351 | cgcgtctgcg | tctggctggc | tggcataaat | atctcactcg | caatcaaatt | 1400 |
1401 | cagccgatag | cggaacggga | aggcgactgg | agtgccatgt | ccggttttca | 1450 |
1451 | acaaaccatg | caaatgctga | atgagggcat | cgttcccact | gcgatgctgg | 1500 |
1501 | ttgccaacga | tcagatggcg | ctgggcgcaa | tgcgcgccat | taccgagtcc | 1550 |
1551 | gggctgcgcg | ttggtgcgga | tatctcggta | gtgggatacg | acgataccga | 1600 |
1601 | agacagctca | tgttatatcc | cgccgttaac | caccatcaaa | caggattttc | 1650 |
1651 | gcctgctggg | gcaaaccagc | gtggaccgct | tgctgcaact | ctctcagggc | 1700 |
1701 | caggcggtga | agggcaatca | gctgttgccc | gtctcactgg | tgaaaagaaa | 1750 |
1751 | aaccaccctg | gcgcccaata | cgcaaaccgc | ctctccccgc | gcgttggccg | 1800 |
1801 | attcattaat | gcagctggca | cgacaggttt | cccgactgga | aagcgggcag | 1850 |
1851 | tgagcgcaac | gcaattaatg | taagttagct | cactcattag | gcaccgggat | 1900 |
1901 | ctcgaccgat | gcccttgaga | gccttcaacc | cagtcagctc | cttccggtgg | 1950 |
1951 | gcgcggggca | tgactatcgt | cgccgcactt | atgactgtct | tctttatcat | 2000 |
2001 | gcaactcgta | ggacaggtgc | cggcagcgct | ctgggtcatt | ttcggcgagg | 2050 |
2051 | accgctttcg | ctggagcgcg | acgatgatcg | gcctgtcgct | tgcggtattc | 2100 |
2101 | ggaatcttgc | acgccctcgc | tcaagccttc | gtcactggtc | ccgccaccaa | 2150 |
2151 | acgtttcggc | gagaagcagg | ccattatcgc | cggcatggcg | gccccacggg | 2200 |
2201 | tgcgcatgat | cgtgctcctg | tcgttgagga | cccggctagg | ctggcggggt | 2250 |
2251 | tgccttactg | gttagcagaa | tgaatcaccg | atacgcgagc | gaacgtgaag | 2300 |
2301 | cgactgctgc | tgcaaaacgt | ctgcgacctg | agcaacaaca | tgaatggtct | 2350 |
2351 | tcggtttccg | tgtttcgtaa | agtctggaaa | cgcggaagtc | agcgccctgc | 2400 |
2401 | accattatgt | tccggatctg | catcgcagga | tgctgctggc | taccctgtgg | 2450 |
2451 | aacacctaca | tctgtattaa | cgaagcgctg | gcattgaccc | tgagtgattt | 2500 |
2501 | ttctctggtc | ccgccgcatc | cataccgcca | gttgtttacc | ctcacaacgt | 2550 |
2551 | tccagtaacc | gggcatgttc | atcatcagta | acccgtatcg | tgagcatcct | 2600 |
2601 | ctctcgtttc | atcggtatca | ttacccccat | gaacagaaat | cccccttaca | 2650 |
2651 | cggaggcatc | agtgaccaaa | caggaaaaaa | ccgcccttaa | catggcccgc | 2700 |
2701 | tttatcagaa | gccagacatt | aacgcttctg | gagaaactca | acgagctgga | 2750 |
2751 | cgcggatgaa | caggcagaca | tctgtgaatc | gcttcacgac | cacgctgatg | 2800 |
2801 | agctttaccg | cagctgcctc | gcgcgtttcg | gtgatgacgg | tgaaaacctc | 2850 |
2851 | tgacacatgc | agctcccgga | gacggtcaca | gcttgtctgt | aagcggatgc | 2900 |
2901 | cgggagcaga | caagcccgtc | agggcgcgtc | agcgggtgtt | ggcgggtgtc | 2950 |
2951 | ggggcgcagc | catgacccag | tcacgtagcg | atagcggagt | gtatactggc | 3000 |
3001 | ttaactatgc | ggcatcagag | cagattgtac | tgagagtgca | ccatatatgc | 3050 |
3051 | ggtgtgaaat | accgcacaga | tgcgtaagga | gaaaataccg | catcaggcgc | 3100 |
3101 | tcttccgctt | cctcgctcac | tgactcgctg | cgctcggtcg | ttcggctgcg | 3150 |
3151 | gcgagcggta | tcagctcact | caaaggcggt | aatacggtta | tccacagaat | 3200 |
3201 | caggggataa | cgcaggaaag | aacatgtgag | caaaaggcca | gcaaaaggcc | 3250 |
3251 | aggaaccgta | aaaaggccgc | gttgctggcg | tttttccata | ggctccgccc | 3300 |
3301 | ccctgacgag | catcacaaaa | atcgacgctc | aagtcagagg | tggcgaaacc | 3350 |
3351 | cgacaggact | ataaagatac | caggcgtttc | cccctggaag | ctccctcgtg | 3400 |
3401 | cgctctcctg | ttccgaccct | gccgcttacc | ggatacctgt | ccgcctttct | 3450 |
3451 | cccttcggga | agcgtggcgc | tttctcatag | ctcacgctgt | aggtatctca | 3500 |
3501 | gttcggtgta | ggtcgttcgc | tccaagctgg | gctgtgtgca | cgaacccccc | 3550 |
3551 | gttcagcccg | accgctgcgc | cttatccggt | aactatcgtc | ttgagtccaa | 3600 |
3601 | cccggtaaga | cacgacttat | cgccactggc | agcagccact | ggtaacagga | 3650 |
3651 | ttagcagagc | gaggtatgta | ggcggtgcta | cagagttctt | gaagtggtgg | 3700 |
3701 | cctaactacg | gctacactag | aaggacagta | tttggtatct | gcgctctgct | 3750 |
3751 | gaagccagtt | accttcggaa | aaagagttgg | tagctcttga | tccggcaaac | 3800 |
3801 | aaaccaccgc | tggtagcggt | ggtttttttg | tttgcaagca | gcagattacg | 3850 |
3851 | cgcagaaaaa | aaggatctca | agaagatcct | ttgatctttt | ctacggggtc | 3900 |
3901 | tgacgctcag | tggaacgaaa | actcacgtta | agggattttg | gtcatgaaca | 3950 |
3951 | ataaaactgt | ctgcttacat | aaacagtaat | acaaggggtg | ttatgagcca | 4000 |
4001 | tattcaacgg | gaaacgtctt | gctctaggcc | gcgattaaat | tccaacatgg | 4050 |
4051 | atgctgattt | atatgggtat | aaatgggctc | gcgataatgt | cgggcaatca | 4100 |
4101 | ggtgcgacaa | tctatcgatt | gtatgggaag | cccgatgcgc | cagagttgtt | 4150 |
4151 | tctgaaacat | ggcaaaggta | gcgttgccaa | tgatgttaca | gatgagatgg | 4200 |
4201 | tcagactaaa | ctggctgacg | gaatttatgc | ctcttccgac | catcaagcat | 4250 |
4251 | tttatccgta | ctcctgatga | tgcatggtta | ctcaccactg | cgatccccgg | 4300 |
4301 | gaaaacagca | ttccaggtat | tagaagaata | tcctgattca | ggtgaaaata | 4350 |
4351 | ttgttgatgc | gctggcagtg | ttcctgcgcc | ggttgcattc | gattcctgtt | 4400 |
4401 | tgtaattgtc | cttttaacag | cgatcgcgta | tttcgtctcg | ctcaggcgca | 4450 |
4451 | atcacgaatg | aataacggtt | tggttgatgc | gagtgatttt | gatgacgagc | 4500 |
4501 | gtaatggctg | gcctgttgaa | caagtctgga | aagaaatgca | taaacttttg | 4550 |
4551 | ccattctcac | cggattcagt | cgtcactcat | ggtgatttct | cacttgataa | 4600 |
4601 | ccttattttt | gacgagggga | aattaatagg | ttgtattgat | gttggacgag | 4650 |
4651 | tcggaatcgc | agaccgatac | caggatcttg | ccatcctatg | gaactgcctc | 4700 |
4701 | ggtgagtttt | ctccttcatt | acagaaacgg | ctttttcaaa | aatatggtat | 4750 |
4751 | tgataatcct | gatatgaata | aattgcagtt | tcatttgatg | ctcgatgagt | 4800 |
4801 | ttttctaaga | attaattcat | gagcggatac | atatttgaat | gtatttagaa | 4850 |
4851 | aaataaacaa | ataggggttc | cgcgcacatt | tccccgaaaa | gtgccacctg | 4900 |
4901 | aaattgtaaa | cgttaatatt | ttgttaaaat | tcgcgttaaa | tttttgttaa | 4950 |
4951 | atcagctcat | tttttaacca | ataggccgaa | atcggcaaaa | tcccttataa | 5000 |
5001 | atcaaaagaa | tagaccgaga | tagggttgag | tgttgttcca | gtttggaaca | 5050 |
5051 | agagtccact | attaaagaac | gtggactcca | acgtcaaagg | gcgaaaaacc | 5100 |
5101 | gtctatcagg | gcgatggccc | actacgtgaa | ccatcaccct | aatcaagttt | 5150 |
5151 | tttggggtcg | aggtgccgta | aagcactaaa | tcggaaccct | aaagggagcc | 5200 |
5201 | cccgatttag | agcttgacgg | ggaaagccgg | cgaacgtggc | gagaaaggaa | 5250 |
5251 | gggaagaaag | cgaaaggagc | gggcgctagg | gcgctggcaa | gtgtagcggt | 5300 |
5301 | cacgctgcgc | gtaaccacca | cacccgccgc | gcttaatgcg | ccgctacagg | 5350 |
5351 | gcgcgtccca | ttcgcca | 5367 |
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