- 询价
- TIANDZ
- 中国
- 2368
- 2025年07月08日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
pCW-LIC
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: pCW-LIC
其他名称:
质粒大小（bp）: 6980
抗性: Ampicillin
质粒类型: Bacterial Expression
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
质粒图谱:
质粒序列:
>Author sequence
gcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgacc
gctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccg
gctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcga
ccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccct
ttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatct
cgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta
acaaaaatttaacgcgaattttaacaaaatattaacgtttacaatcaggtggcacttttcggggaaatgt
gcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccc
tgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattc
ccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctga
agatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagtttt
cgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtg
ttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcacc
agtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagt
gataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcaca
acatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacga
gcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttact
ctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcgg
cccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgc
agcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatg
gatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaag
tttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcct
ttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaa
aagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccac
cgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcag
cagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgta
gcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtc
ttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtg
cacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagc
gccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgca
cgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttga
gcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggccttttta
cggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggata
accgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagt
gagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgc
atatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcg
ctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtc
tgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcacc
gtcatcaccgaaacgcgcgaggcagaacgccatcaaaaataattcgcgtctggccttcctgtagccagct
ttcatcaacattaaatgtgagcgagtaacaacccgtcggattctccgtgggaacaaacggcggattgacc
gtaatgggataggttacgttggtgtagatgggcgcatcgtaaccgtgcatctgccagtttgaggggacga
cgacagtatcggcctcaggaagatcgcactccagccagctttccggcaccgcttctggtgccggaaacca
ggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgcta
ttacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagt
cacgacgttgtaaaacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgtt
atccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagt
gagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctg
cattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttc
accagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtcca
cgctggtttgccccagcaggcgaaaatcctgtttgatggtggttgacggcgggatataacatgagctgtc
ttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgc
attgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcattt
gcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgatt
gcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaac
agcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggaga
aaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagc
ttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgaga
agattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcac
ccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggt
ggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagc
tccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcggg
aaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccac
cctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcaccattcgatggtgtcc
tggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcactc
attaggcaccccaggctttacactttatgcttccggctcgtataatgtgtggaattgtgagcggataaca
atttcacacaggaaacaggatcgatccatcgatgagcttactccccatccccctgttgacaattaatcat
cggctcgtataatgtgtggaattgtgagcggataacaatttcacacaggaaacaggatcagcttactccc
catccccctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttcaca
caggaaacaggatccatcgatgcttaggaggtcatatgattatgagttctcctcctgaaagatccataac
ttcgtatagcatacattatacgaagttatgcggccgcgacgtccacatatacctgccgttcactattatt
tagtgaaatgagatattatgatattttctgaattgtgattaaaaaggcaactttatgcccatgcaacaga
aactataaaaaatacagagaatgaaaagaaacagatagattttttagttctttaggcccgtagtctgcaa
atccttttatgattttctatcaaacaaaagaggaaaatagaccagttgcaatccaaacgagagtctaata
gaatgaggtcgaaaagtaaatcgcgcgggtttgttactgataaagcaggcaagacctaaaatgtgtaaag
ggcaaagtgtatactttggcgtcaccccttacatattttaggtctttttttattgtgcgtaactaacttg
ccatcttcaaacaggagggctggaagaagcagaccgctaacacagtacataaaaaaggagacatgaacga
tgaacatcaaaaagtttgcaaaacaagcaacagtattaacctttactaccgcactgctggcaggaggcgc
aactcaagcgtttgcgaaagaaacgaaccaaaagccatataaggaaacatacggcatttcccatattaca
cgccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaagttcctgagttcgattcgt
ccacaattaaaaatatctcttctgcaaaaggcctggacgtttgggacagctggccattacaaaacgctga
cggcactgtcgcaaactatcacggctaccacatcgtctttgcattagccggagatcctaaaaatgcggat
gacacatcgatttacatgttctatcaaaaagtcggcgaaacttctattgacagctggaaaaacgctggcc
gcgtctttaaagacagcgacaaattcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtc
aggttcagccacatttacatctgacggaaaaatccgtttattctacactgatttctccggtaaacattac
ggcaaacaaacactgacaactgcacaagttaacgtatcagcatcagacagctctttgaacatcaacggtg
tagaggattataaatcaatctttgacggtgacggaaaaacgtatcaaaatgtacagcagttcatcgatga
aggcaactacagctcaggcgacaaccatacgctgagagatcctcactacgtagaagataaaggccacaaa
tacttagtatttgaagcaaacactggaactgaagatggctaccaaggcgaagaatctttatttaacaaag
catactatggcaaaagcacatcattcttccgtcaagaaagtcaaaaacttctgcaaagcgataaaaaacg
cacggctgagttagcaaacggcgctctcggtatgattgagctaaacgatgattacacactgaaaaaagtg
atgaaaccgctgattgcatctaacacagtaacagatgaaattgaacgcgcgaacgtctttaaaatgaacg
gcaaatggtacctgttcactgactcccgcggatcaaaaatgacgattgacggcattacgtctaacgatat
ttacatgcttggttatgtttctaattctttaactggcccatacaagccgctgaacaaaactggccttgtg
ttaaaaatggatcttgatcctaacgatgtaacctttacttactcacacttcgctgtacctcaagcgaaag
gaaacaatgtcgtgattacaagctatatgacaaacagaggattctacgcagacaaacaatcaacgtttgc
gcctagcttcctgctgaacatcaaaggcaagaaaacatctgttgtcaaagacagcatccttgaacaagga
caattaacagttaacaaataaaaacgcaaaagaaaatgccgatatcctattggcattgacgtcaggtggc
acttttcgaggagatcatgcacatgatgacgaagcttatcgatgataagctgtcaaacatgagcagatct
gagcccgcctaatgagcgggcttttttttcagatctgcttgaagacgaaagggcctcgtgatacgcctat
ttttataggttaatgtcatgataataatggtttcttaggtgctcgtcaaa
>26098 with LacI-R
ACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGT
CCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCAC
TCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAA
CAATTTCACACAGGAAACAGGATCGATCCATCGATGAGCTTACTCCCCATCCCCCTGTTGACAATTAATC
ATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGGATCAGCTTACTC
CCCATCCCCCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCA
CACAGGAAACAGGATCCATCGATGCTTAGGAGGTCATATGATTATGAGTTCTCCTCCTGAAAGATCCATA
ACTTCGTATAGCATACATTATACGAAGTTATGCGGCCGCGACGTCCACATATACCTGCCGTTCACTATTA
TTTAGTGAAATGAGATATTATGATATTTTCTGAATTGTGATTAAAAAGGCAACTTTATGCCCATGCAACA
GAAACTATAAAAAATACAGAGAATGAAAAGAAACAGATAGATTTTTTAGTTCTTTAGGCCCGTAGTCTGC
AAATCCTTTTATGATTTTCTATCAAACAAAAGAGGAAAATAGACCAGTTGCAATCCAAACGAGAGTCTAA
TAGAATGAGGTCGAAAAGTAAATCGCGCGGGTTTGTTACTGATAAAGCAGGCAAGACCTAAAATGTGTAA
AGGGCAAAGTGTATACTTTGGCGTCA
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文献和实验The protocol for LIC by Exonuclease III
The protocol for LIC by Exonuclease III 梁耀极 1. Design the primers with 15-bp overlap; 2. Digest the vector by proper restriction enzyme; For getting high quality vectors, there are some good advices as follows: 1) The restriction
（ doisyno-lic acid）系物质，或雌烷的 D环变为六环的同型雌酮等。有关这些结构不同而作用相同的原因目前尚不清楚。雌激素的鉴定法有 Allen-Doisy试验和子宫重量法。由于试验方法和给予激素方法的差异，所得结果不一定相同。都是以 0.1微克纯雌酮所引起的动作用（阴道上皮角质化）作为一国际单位（ IU）。而对苯甲酸盐的制剂，则从雌二醇的苯甲酸盐 0.1微克作为标准。
High-Throughput Cloning for Proteomics Research
Ligation-independent cloning (LIC) is a simple, rapid, and efficient method for high-throughput cloning. In this system, linear plasmid vector and insert DNA are treated to generate complementary single-stranded overhangs that anneal
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