pCPOT

Restriction digests of the clone give the following sizes (kb): PstI–14, 4.3; AccI–3.3, 2.7, 2.7, 2.5, 2.5, 2.2, 1.5, 1.3; BglI–11.0, 4.5, 2.5; EcoRI–4.2, 3.3, 2.9, 2.9, 1.7, 1.3, 1.3. (ATCC staff) The presence of the POT1 gene ensures stable maintenance of the plasmid in yeast during growth on nonselective medium using glucose as a carbon source. [1] An episomal Saccharomyces cerevisiae/E.coli shuttle expression vector containing 2 micron circle, pBR322 replication and selection, and Schizosaccharomyces pombe sequences encoding triose phosphate isomerase (POT1). [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli.