pGW7

名称	编号	规格	价格	手册
pGW7

粒编号:
质粒名称: pGW7
其他名称:
质粒大小（bp）: 8002
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Created by Moore, July 1995, under contract with NCBI. Restriction digests of the clone give the following sizes (kb): EcoRI–8.0; BamHI–8.0; EcoRI/BamHI–4.0 (doublet). (ATCC staff) Expression vector permitting thermal regulation of expression from the PL promoter. [1] Constructed from a BamHI/EcoRI fragment of pBR322 and a 3.96 kb BamHI/EcoRI fragment from bacteriophage lambda, containing N, rexB, rexA, cI and modified by deleting a 711 bp BglII fragment containing cII and portions of the cro and O genes. [1] The order of the major features in the plasmid is: EcoRI – partial O – partial cro – lambda R promoter – lambda M promoter – cIts – rexA – rexB – lambda L promoter – HpaI / N – BamHI – SphI – SalI – pMB1 ori – ampR. [1] The vector contains the following restriction sites (bp from the EcoRI site): BglII–352, 2744; HindIII–871, 996, 1560; PstI–1454, 7190; HpaI–3196; BstXI–3853; BamHI–3956; SphI–4143; SalI–4232; AvaI–5006; PvuII–5647; PvuI–7316. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli RR1. Related vectors: pBR322, lambda.
质粒图谱: