- ￥980
- EASYBIO
- BE3173-50
- 2026年03月20日
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- 详细信息
- 文献和实验
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
Cleaved PARP Mouse Monoclonal Antibody
- 规格：
支
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.
Product Name:Cleaved PARP Mouse Monoclonal Antibody
Clone Number: M4
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000-3,000
Optimal dilutions should be determined by the end user.
Specificity: Antibody can detects endogenous pro and active PARP protein.
Alternative Names:PARP-1, Poly(ADP ribose) polymerase 1, sPARP1,ADPRT1, ADP ribosyltransferase NAD(+)
Molecular Weight:116KD（Pro） 89KD（Active）
Form: Liquid
Reactivity:H
Western blot analysis of 1) Jurkat ,2) Hela, with BE3173 diluted at 1:2,000
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文献和实验Monoclonal Antibody Production
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Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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