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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
多克隆
- 抗体英文名:
α-Skeletal Muscle Actin Rabbit Polyclonal Antibody
- 规格:
支
Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Product Name:α Skeletal Muslce Actin Rabbit Polyclonal Antibody
Isotype: IgG
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000-2,000
Optimal dilutions should be determined by the end user.
Specificity: TheαSkeletal Muscle Actin antibody can detects endogenousαSkeletal Muscle Actin protein.
Alternative Names:ACTA antibody, ACTA1 antibody, ACTC antibody, ACTC1 antibody, Actin alpha cardiac muscle antibody, ACTS antibody, Alpha actin 1 antibody, Cardiac muscle alpha actin 1 antibody, Skeletal muscle alpha actin 1 antibody
Form: Liquid
Reactivity:H, R, M
Molecular Weight: 42KD

Western blot analysis of 1) Hela, 2)3T3, 3) Rat Kidney tissue with BE3197 diluted at 1:2,000.
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文献和实验Preparation of Conventional Actin from Skeletal Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866. 1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min. Use a big stir bar or overhead stirrer. Buffer G: 0.2 mM ATP, 0.5 mM DTT, 0.2 mM
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
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