Caspase 9 Mouse Monoclonal An

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Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Product Name:Caspase 9 Mouse Monoclonal Antibody

Clone Number: BE-21

Isotype: IgG1

Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol

Storage instructions:-20°C. Do not aliquot the antibody

Recommended dilutions: WB: 1:1,000-5,000 IP: 1:200 IF: 1:100-200

Optimal dilutions should be determined by the end user.

Specificity: Antibody can detects endogenous Caspase 9 protein.

Alternative Names:APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody

Molecular Weight:47KD(H), 51KD(R, M)

Form: Liquid

Reactivity:H, R, M

Western blot analysis of Hela with Caspase 9 (BE-21) Mouse mAb dilutedat

1）1:2,000

2）1:5,000

Western blot analysis of 1）Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 (BE-21) Mouse mAb diluted at 1:3,000

1、Input: Hela Cell Lysate

2、IP product: IP dilute 1:200

Western blot analysis:primary antibody:BE3111 1:1,000

Secondary antibody: Goat anti-Mouse IgG, Light chain specific(BE0105), 1:5,000

IF analysis of Hela with BE3111(Left) and DAPI (Right) diluted at 1:100.