- ￥1480
- EASYBIO
- BE2012-100
- 2025年12月29日
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
C-Myc-Tag(HRP Conjugated) Mouse Monoclonal Antibody
- 规格：
支
c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. Myc protein is a transcription factor that activates expression of a great number of genes through binding on consensus sequences (Enhancer Box sequences (E-boxes)) and recruiting histone acetyltransferases (HATs). A recent study demonstrated that temporary inhibition of Myc selectively kills mouse lung cancer cells, making it a potential cancer drug target.
Product Name:c-Myc Mouse Monoclonal Antibody-HRP conjugated
Isotype: IgG1
Clone Number: 4E9
Storage Buffer :PBS, pH 7.4, containing 0.02% kathonas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000-3,000 ELISA: Use at an assay dependent dilution
Optimal dilutions should be determined by the end user.
Specificity: The C-Myc tag antibody can detect C-Myc fusion proteins.
Alternative Names:Myc tag, Myc protein, Myc epitope tag, Myc2
Form: Liquid
Reactivity:ALL
Western blot analysis of c-Myc Recombinant protein with BE2012 diluted at 1:2,000.
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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