HA-Tag Mouse Monoclonal Antibo

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HA tag Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection. HA tag antibody can detect HA tags in internal, C-terminal, or N-terminal recombinant proteins.

Product Name:HA-tag mouse monoclonal antibody

Clone Number: 2B11

Isotype: IgG1

Immunogen: A synthetic peptide YPYDVPDYAcoupled to KLH.

Tested applications and dilutions: WB 1:5000-2000 IP 1:200 IF 1:1000

Produce anti- HA antibody affinity column purify HA recombinant proteins

Form:Liquid

Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol

Storage: HAantibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

A: HEK 293 cell lysate without transfected

B: HEK 293 cell lysate transfected with HA-tag protein

C: IP (HEK 293 cell without transfected + anti- HA mAb 4℃overnight)

D: IP (PBS + anti- HA mAb 4℃overnight)

E: IP (HEK293 transfected+protein G+anti- HA mAb 4℃overnight)

F: IP (HEK293 transfected+RIPA+Protein G 4℃overnight)

G: Multi Tag Recombinant protein (expressed in E.coli)

TP=Target Protein HC=Heavy chain LC=Light Chain

IF analysis of 293T cells transfected with a HA-tagged protein, using easybio HA-Tag (2B11) Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA)