Product Name: Plant-Actin Rabbit Polyclonal antibody
Immunogen: Synthetic peptide derived from within N-terminal residues of Oryza sativa actin (NP_001066233) conjugated to KLH
Purification: peptide affinity purification
Tested applications: WB
Recommended dilutions: WB: 1:2000~1:5000
Optimal dilutions/concentrations should be determined by the user.
Molecular Weight: 43KD
Form: Liquid
Concentration: 0.5mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: Plant-Actin PAB is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Reactivity: ArabidopsisNicotiana tabacum Oryza sativa Hordeum vulgare Predicted to work with: Brassica napus Gossypium herbaceum Solanum tuberosum Setaria italic Triticum monococcum Brachypodium distachyon Chrysanthemum seticuspe Glycine max Mangifera Thellungiella halophila Zoysia japonica Isatis tinctoria
Western blot analysis using primary antibody Plant-actin PAB Loading Control (EASYBIO BE0027). Lane1 lysates of Nicotiana tabacum 1:2000 Lane2 lysates of Oryza sativa 1:2000 Lane3 lysates of Nicotiana tabacum 1:5000 Lane4 lysates of Oryza sativa 1:5000 Second antibody using 1:10000 (EASYBIO BE0101) ECL using Millipore(Merck)WBKLS0500