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- EASYBIO
- BE2062-10
- 2026年04月03日
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- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
His-Tag(HRP Conjugated) Mouse Monoclonal Antibody
- 规格：
10μl/支
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Product Name:His-Tag Mouse Monoclonal Antibody-HRP conjugated
Isotype: IgG1
Clone Number: 6E8
Storage Buffer :PBS, pH 7.4, 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:3,000-10,000 ELISA: Use at an assay dependent dilution
Optimal dilutions should be determined by the end user.
Specificity: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
Form: Liquid
Reactivity:ALL
Western blot analysis of His recombinant protein with His Mouse mAb(6E8) HRP Conjugated diluted at
1）1:10,000
2）1:20,000
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文献和实验Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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