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- EASYBIO
- BE0031-10
- 2025年12月24日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
α-tubulin Mouse Monoclonal Antibody
- 规格：
10μl/支
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin
Product Name: α-tubulin Mouse Monoclonal Antibody
Isotype: IgG1
Clone Number: 9F12
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:5,000-10,000 IP: 1:200 IF: 1:100-200
Optimal dilutions should be determined by the end user.
Specificity: The α-tubulin antibody can detects endogenousα-tubulin protein
Alternative Names:alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain
Molecular Weight: 52KD
Form: Liquid
Reactivity:H, R, M, zebra fish
Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with BE0031 diluted at 1:5,000
1、Input: Mouse Brain Tissue Lysate
2、IP product: IP dilute 1:200Western blot analysis: primaryantibody:BE0031 1:5,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific (BE0105), 1:5,000
IF analysis of Hela with BE0031(Left) and DAPI (Right) diluted at 1:100.
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文献和实验[2]A lncRNA fine-tunes salicylic acid biosynthesis to balance plant immunity and growth.Ningkun Liu, Yanzhuo Xu, Qi Li, Yuxin Cao, Dechang Yang, Shasha Liu, Xiaokang Wang, Yingjie Mi, Yang Liu, Chenxi Ding, Yan Liu, Yong Li, Yao-Wu Yuan, Ge Gao, Jinfeng Chen, Weiqiang Qian, Xiaoming Zhang.Cell Host & Microbe.2022(7)
Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
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