- ￥1480
- EASYBIO
- BE2074-100
- 2025年12月29日
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- 文献和实验
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
V5-Tag Mouse Mixture Monoclonal Antibody
- 规格：
支
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein.
Product Name:V5-Tag Mouse Mixture Monoclonal Antibody
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:5,000 IP: 1:200 IF: 1:200
Optimal dilutions should be determined by the end user.
Specificity: The V5 tag antibody can recognize C-terminal, internal, and N-terminal V5-tag fusion proteins.
Alternative Names:GKPIPNPLLGLDST epitope tag, protein rev, V5 epitope tag
Form: Liquid
Reactivity:ALL
Western blot analysis of His-MBP-V5 recombinant protein with BE2074 diluted at 1:10,000.
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Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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