- ￥200
- EASYBIO
- BE0025-10
- 2026年01月23日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
β-Tubulin Mouse Monoclonal Antibody
- 规格：
10μl/支
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulinmay not be stable in certain cells. For example, expression of β-Tubulinin adipose tissue is very low and therefore β-Tubulinshould not be used as loading control for these tissues.
Product Name:β-Tubulinmouse monoclonal antibody
Clone Number: 6G4
Isotype: IgG1
Immunogen: Full-lengthβ-Tubulin protein of human.
Tested applications: WB, IHC. Not yet tested in other applications.
Recommended dilutions: WB: 1:2000-5000 IHC: 1:200IF: 1:100-200
Optimal dilutions/concentrations should be determined by the end user.
Molecular Weight: 55KD
Form:Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: β-Tubulin antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Reactivity: H, R, M, Mk,Zebrafish
Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(6G4) diluted at 1:5000.
Immunohistochemistry Staining of Human colon tissue with β-Tubulin mouse mAb(6G4) diluted at 1:200.
IF analysis of Hela with BE0025(Left) and DAPI (Right) diluted at 1:100.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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