- ￥880
- EASYBIO
- BE3311-100
- 2025年12月29日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
EBFP-Tag Mouse Monoclonal Antibody
- 规格：
支
Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.
Product Name: EBFP Mouse Mixture Monoclonal Antibody
Isotype: IgG
Storage Buffer : PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:5,000 IHC: 1:200 IP: 1:200
Optimal dilutions should be determined by the end user.
Specificity: The EBFP Mouse Polyclonal Antibody can detects EBFP & EBFP Tag protein.
Alternative Names: BFP, EBFP, Blue Fluorescent Protein, Enhanced Blue Fluorescent Protein
Form: Liquid
Reactivity: ALL
Western blot analysis of recombinant EBFP protein with BE3311 diluted at 1:5,000.
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
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