- ￥1480
- EASYBIO
- BE3182-100
- 2026年01月23日
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
α skeletal muscle actin Mouse Monoclonal Antibody
- 规格：
支
Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Product Name:α Skeletal Muslce Actin Mouse Monoclonal Antibody
Clone Number: 5B10
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:5,000-10,000 IP: 1:200
Optimal dilutions should be determined by the end user.
Specificity: Antibody can detects endogenous αSkeletal Muscle Actin protein.
Alternative Names:ACTA antibody, ACTA1 antibody, ACTC antibody, ACTC1 antibody, Actin alpha cardiac muscle antibody, ACTS antibody, Alpha actin 1 antibody, Cardiac muscle alpha actin 1 antibody, Skeletal muscle alpha actin 1 antibody
Molecular Weight:42KD
Form: Liquid
Reactivity:H, R, M
Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue with BE3182 diluted at 1:20,000.
1、Input: Mouse Brain Tissue Lysate 2、IP product: IP dilute 1:200
Western blot analysis: primary antibody:BE3182 1:10,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific (BE0105), 1:5,000
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
describes vital staining of neuromuscular junctions in the mouse triangularis sterni muscle in one incubation step, combining presynaptic, motor nerve terminal staining with the styryl dye FM1?43, which labels recycling synaptic vesicles, and TRITC
Determination of Muscle Fiber Type in Rodents
2: Histological Staining for Succinate Dehydrogenase (SDH) Alternate Protocol 1: Fiber Typing of Mouse Skeletal Muscle Using Antibodies Against Specific Myosin Isoforms Commentary Literature Cited
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