- 询价
- TIANDZ
- 中国
- 1978
- 2025年07月13日
14 年钻石会员
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
pBD9
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: pBD9
其他名称:
质粒大小（bp）: 8276
抗性:
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Created by Moore, July 1995, under contract with NCBI. A general purpose plasmid vector for cloning in Bacillus subtilis. (ATCC staff) This plasmid is maintained at a high copy number. Medium is 1179 V Y medium. Hosts: Bacillus subtilis BD170, Bacillus, Staphylococcus aureus. Related vectors: pUB110, pE194, pBD8, pBD6, pBD10, pBD11, pBD12, pBD20, pBD35.
质粒图谱:
质粒序列:
| 1 | gaattcctta | gtgctttcat | agattaaact | cacatcacgc | tttaaatcgc | 50 |
| 51 | ttattttaga | ctttaaagac | ttgttttctt | caagcaactc | attataatca | 100 |
| 101 | tttacatttt | cattaaatcg | ctctacaaga | ccactatatt | tttctttaac | 150 |
| 151 | ttgcccatgt | tctttactta | attttttata | ttctctcgcc | atatcagtac | 200 |
| 201 | tcatgagatt | tctaacatgc | tgttttaacc | tatcgttatc | tctcgcagca | 250 |
| 251 | gtcactaagt | ttttataatc | acgctccgat | ataacaacat | ttttggttgg | 300 |
| 301 | tttcttttct | gttttcatta | tttcttttcc | caaaccaaac | atggactttt | 350 |
| 351 | cacccgttgg | cacttcaaca | cttttcatgt | gtcgtttcgc | tggtacttct | 400 |
| 401 | aaatctgatt | taactttatc | gctataagca | gtccattcat | cttttttaac | 450 |
| 451 | tgctaaattt | ttttctagaa | aatgaatacg | gtaaggaaaa | tatgctgtat | 500 |
| 501 | gcgactgtcc | atctggatga | aagagtccca | catatgcact | ttggttttgt | 550 |
| 551 | ccctttaaca | gaggacggga | gattgtctgc | aaaagaacag | ttaggcaaca | 600 |
| 601 | agaaagactt | tactcaatta | caagatagat | ttaatgagta | tgtgaatgag | 650 |
| 651 | aaaggttatg | aacttgaaag | aggcacgtcc | aaagaggtta | cagaacgaga | 700 |
| 701 | acataaagcg | atggatcagt | acaagaaaga | tactgtattt | cataaacagg | 750 |
| 751 | aactgcaaga | agttaaggat | gagttacaga | aggcaaataa | gcagttacag | 800 |
| 801 | agtggaatag | agcatatgag | gtctacgaaa | ccctttgatt | atgaaaatga | 850 |
| 851 | gcgtacaggt | ttgttctctg | gacgtgaaga | gactggtaga | aagatattaa | 900 |
| 901 | ctgctgatga | atttgaacgc | ctgcaagaaa | caatctcttc | tgcagaacgg | 950 |
| 951 | attgttgatg | attacgaaaa | tattaagagc | acagactatt | acacagaaaa | 1000 |
| 1001 | tcaagaatta | aaaaaacgta | gagagagttt | gaaagaagta | gtgaatacat | 1050 |
| 1051 | ggaaagaggg | gtatcacgaa | aaaagtaaag | aggttaataa | attaaagcga | 1100 |
| 1101 | gagaatgata | gtttgaatga | gcagttgaat | gtatcagaga | aatttcaagc | 1150 |
| 1151 | tagtacagtg | actttatatc | gtgctgcgag | ggcgaatttc | cctgggtttg | 1200 |
| 1201 | agaaagggtt | taataggctt | aaagagaaat | tctttaatga | ttccaaattt | 1250 |
| 1251 | gagcgtgtgg | gacagtttat | ggatgttgta | caggataatg | tccagaaggt | 1300 |
| 1301 | cgatagaaag | cgtgagaaac | agcgtacaga | cgatttagag | atgtagaggt | 1350 |
| 1351 | acttttatgc | cgagaaaact | ttttgcgtgt | gacagtcctt | aaaatatact | 1400 |
| 1401 | tagagcgtaa | gcgaaagtag | tagcgacagc | tattaacttt | cggttgcaaa | 1450 |
| 1451 | gctctaggat | ttttaatgga | cgcagcgcat | cacacgcaaa | aaggaaattg | 1500 |
| 1501 | gaataaatgc | gaaatttgag | atgttaatta | aagacctttt | tgaggtcttt | 1550 |
| 1551 | ttttcttaga | tttttggggt | tatttagggg | agaaaacata | ggggggtact | 1600 |
| 1601 | acgacctccc | ccctaggtgt | ccattgtcca | ttgtccaaac | aaataaataa | 1650 |
| 1651 | atattgggtt | tttaatgtta | aaaggttgtt | ttttatgtta | aagtgaaaaa | 1700 |
| 1701 | aacagatgtt | gggaggtaca | gtgatggttg | tagatagaaa | agaagagaaa | 1750 |
| 1751 | aaagttgctg | ttactttaag | acttacaaca | gaagaaaatg | agatattaaa | 1800 |
| 1801 | tagaatcaaa | gaaaaatata | atattagcaa | atcagatgca | accggtattc | 1850 |
| 1851 | taataaaaaa | atatgcaaag | gaggaatacg | gtgcatttta | aacaaaaaaa | 1900 |
| 1901 | gatagacagc | actggcatgc | tgcctatcta | tgactaaatt | ttgttaagtg | 1950 |
| 1951 | tattagcacc | gttattatat | catgagcgaa | aatgtaataa | aagaaactga | 2000 |
| 2001 | aaacaagaaa | aattcaagag | gacgtaattg | gacatttgtt | ttatatccag | 2050 |
| 2051 | aatcagcaaa | agccgagtgg | ttagagtatt | taaaagagtt | acacattcaa | 2100 |
| 2101 | tttgtagtgt | ctccattaca | tgatagggat | actgatacag | aaggtaggat | 2150 |
| 2151 | gaaaaaagag | cattatcata | ttctagtgat | gtatgagggt | aataaatctt | 2200 |
| 2201 | atgaacagat | aaaaataatt | aacagaagaa | ttgaatgcga | ctattccgca | 2250 |
| 2251 | gattgcagga | agtgtgaaag | gtcttgtgag | atatatgctt | cacatggacg | 2300 |
| 2301 | atcctaataa | atttaaatat | caaaaagaag | atatgatagt | ttatggcggt | 2350 |
| 2351 | gtagatgttg | atgaattatt | aaagaaaaca | acaacagata | gatataaatt | 2400 |
| 2401 | aattaaagaa | atgattgagt | ttattgatga | acaaggaatc | gtagaattta | 2450 |
| 2451 | agagtttaat | ggattatgca | atgaagttta | aatttgatga | ttggttcccg | 2500 |
| 2501 | cttttatgtg | ataactcggc | gtatgttatt | caagaatata | taaaatcaaa | 2550 |
| 2551 | tcggtataaa | tctgaccgat | agattttgaa | tttaggtgtc | acaagacact | 2600 |
| 2601 | cttttttcgc | accagcgaaa | actggtttaa | gccgactgcg | caaaagacat | 2650 |
| 2651 | aatcgattca | caaaaaatag | gcacacgaaa | aacaagttaa | gggatgcagt | 2700 |
| 2701 | ttatgcatcc | cttaacttac | ttattaaata | atttatagct | attgaaaaga | 2750 |
| 2751 | gataagaatt | gttcaaagct | aatattgttt | aaatcgtcaa | ttcctgcatg | 2800 |
| 2801 | ttttaaggaa | ttgttaaatt | gattttttgt | aaatattttc | ttgtattctt | 2850 |
| 2851 | tgttaaccca | tttcataacg | aaataattat | acttttgttt | atctttgtgt | 2900 |
| 2901 | gatattcttg | atttttttct | acttaatctg | ataagtgagc | tattcacttt | 2950 |
| 2951 | aggtttagga | tgaaaatatt | ctcttggaac | catacttaat | atagaaatat | 3000 |
| 3001 | caacttctgc | cattaaaagt | aatgccaatg | agcgttttgt | atttaataat | 3050 |
| 3051 | cttttagcaa | acccgtattc | cacgattaaa | taaatctcat | tagctatact | 3100 |
| 3101 | atcaaaaaca | attttgcgta | ttatatccgt | acttatgtta | taaggtatat | 3150 |
| 3151 | taccatatat | tttataggat | tggtttttag | gaaatttaaa | ctgcaatata | 3200 |
| 3201 | tccttgttta | aaacttggaa | attatcgtga | tcaacaagtt | tattttctgt | 3250 |
| 3251 | agttttgcat | aatttatggt | ctatttcaat | ggcagttacg | aaattacacc | 3300 |
| 3301 | tctttactaa | ttcaagggta | aaatggcctt | ttcctgagcc | gatttcaaag | 3350 |
| 3351 | atattatcat | gttcatttaa | tcttatattt | gtcattattt | tatctatatt | 3400 |
| 3401 | atgttttgaa | gtaataaagt | tttgactgtg | ttttatattt | ttctcgttca | 3450 |
| 3451 | ttataaccct | ctttaatttg | gttatatgaa | ttttgcttat | taacgattca | 3500 |
| 3501 | ttataaccac | ttattttttg | tttggttgat | aatgaactgt | gctgattaca | 3550 |
| 3551 | aaaatactaa | aaatgcccat | attttttcct | ccttataaaa | ttagtataat | 3600 |
| 3601 | tatagcacga | gctctgataa | atatgaacat | gatgagtgat | cgttaaattt | 3650 |
| 3651 | atactgcaat | cggatgcgat | tattgaataa | aagatatgag | agatttatct | 3700 |
| 3701 | aatttctttt | ttcttgtaaa | aaaagaaagt | tcttaaaggt | tttatagttt | 3750 |
| 3751 | tggtcgtaga | gcacacggtt | taacgactta | attacgaagt | aaataagtct | 3800 |
| 3801 | agtgtgttag | actttatgaa | atctatatac | gtttatatat | atttattatc | 3850 |
| 3851 | cggaggtgta | gcatgtctca | ttcaattttg | agggttgcca | gagttaaagg | 3900 |
| 3901 | atcaagtaat | acaaacggga | tacaaagaca | taatcaaaga | gagaataaaa | 3950 |
| 3951 | actataataa | taaagacata | aatcatgagg | aaacatataa | aaattatgat | 4000 |
| 4001 | ttgattaacg | cacaaaatat | aaagtataaa | gataaaattg | atgaaacgat | 4050 |
| 4051 | tgatgagaat | tattcaggga | aacgtaaaat | tcggtcagat | gcaattcgac | 4100 |
| 4101 | atgtggacgg | actggttaca | agtgataaag | atttctttga | tgatttaagc | 4150 |
| 4151 | ggagaagaaa | tagaacgatt | ttttaaagat | agcttggagt | ttctagaaaa | 4200 |
| 4201 | tcaatctctt | tttccaaagt | ttgtttttta | aatttagctg | tctcaatatg | 4250 |
| 4251 | tttacggtca | gagccacgtt | caccacgctt | caactcaaaa | ccctgttttt | 4300 |
| 4301 | tcatatgctc | ggggaattta | tcttgtagcc | ataacagttc | ttgacgatta | 4350 |
| 4351 | aacacatttt | ttccttgcag | ttttccatca | cgcataggca | caacacctaa | 4400 |
| 4401 | atgcatgtga | ggggtttgct | catcattatg | aactgttgca | taagcaatat | 4450 |
| 4451 | tttgcttgcc | atatcgttcg | gaaaataatt | tataactttc | ctcaaaaaat | 4500 |
| 4501 | cgtttttgtt | ctcctggatc | cagttgctca | aaaaaatctc | ggtcagatgt | 4550 |
| 4551 | tactagcaac | tcatttacaa | gaacagcatc | tttcctccgt | ttttcttgta | 4600 |
| 4601 | cctgtttttt | gtgattcaat | aatttctttg | acacgttcgt | tgtaatcaat | 4650 |
| 4651 | atttttatca | tttttcaaat | cataattttc | acgtgttcgc | tcatggtcaa | 4700 |
| 4701 | tatcatcatt | cgttctactt | tttcgctctc | tttgattatg | aaattgcatg | 4750 |
| 4751 | ccttttagtc | cagctgattt | cactttttgc | attctacaaa | ctgcataact | 4800 |
| 4801 | catatgtaaa | tcgctccttt | ttaggtggca | caaatgtgag | gcattttcgc | 4850 |
| 4851 | tctttccggc | aaccacttcc | aagtaaagta | taacacacta | tactttatat | 4900 |
| 4901 | tcataaagtg | tgtgctctgc | gaggctgtcg | gcagtgccga | ccaaaaccat | 4950 |
| 4951 | aaaaccttta | agacctttct | tttttttacg | agaaaaaaga | aacaaaaaaa | 5000 |
| 5001 | cctgccctct | gccacctcag | caaagggggg | ttttgctctc | gtgctcgttt | 5050 |
| 5051 | aaaaatcagc | aagggacagg | tagtattttt | tgagaagatc | actcaaaaaa | 5100 |
| 5101 | tctccacctt | taaacccttg | ccaattttta | ttttgtccgt | tttgtctagc | 5150 |
| 5151 | ttaccgaaag | ccagactcag | caagaataaa | atttttattg | tctttcggtt | 5200 |
| 5201 | ttctagtgta | acggacaaaa | ccactcaaaa | taaaaaagat | acaagagagg | 5250 |
| 5251 | tctctcgtat | cttttattca | gcaatcgcgc | ccgattgctg | aacagattaa | 5300 |
| 5301 | taatagattt | tagcttttta | tttgttgaaa | aaagctaatc | aaattgttgt | 5350 |
| 5351 | cgggatcaat | tactgcaaag | tctcgttcat | cccaccactg | atcttttaat | 5400 |
| 5401 | gatgtattgg | ggtgcaaaat | gcccaaaggc | ttaatatgtt | gatataattc | 5450 |
| 5451 | atcaattccc | tctacttcaa | tgcggcaact | agcagtacca | gcaataaacg | 5500 |
| 5501 | actccgcacc | tgtacaaacc | ggtgaatcat | tactacgaga | gcgccagctt | 5550 |
| 5551 | catcacttgc | ctcccataga | tgaatccgaa | cctcattaca | cattagaact | 5600 |
| 5601 | gcgaatccat | cttcatggtg | aaccaaagtg | aaacctagtt | tatcgcaata | 5650 |
| 5651 | aaaacctata | ctctttttaa | tatccccgac | tggcaatgcc | gggatagact | 5700 |
| 5701 | gtaacattct | cacgcataaa | atcccctttc | attttctaat | gtaaatctat | 5750 |
| 5751 | taccttatta | ttaattcaat | tcgctcataa | ttaatccttt | ttcttattac | 5800 |
| 5801 | gcaaaatggc | ccgatttaag | cacacccttt | attccgttaa | tgcgccatga | 5850 |
| 5851 | cagccatgat | aattactaat | actaggagaa | gttaataaat | acgtaaccaa | 5900 |
| 5901 | catgattaac | aattattaga | ggtcatcgtt | caaaatggta | tgcgttttga | 5950 |
| 5951 | cacatccact | atatatccgt | gtcgttctgt | ccactcctga | atcccattcc | 6000 |
| 6001 | agaaattctc | tagcgattcc | agaagtttct | cagagtcgga | aagttgacca | 6050 |
| 6051 | gacattacga | actggcacag | atggtcataa | cctgaaggaa | gatctgattg | 6100 |
| 6101 | cttaactgct | tcagttaaga | ccgaagcgct | cgtcgtataa | cagatgcgat | 6150 |
| 6151 | gatgcagacc | aatcaacatg | gcacctgcca | ttgctacctg | tacagtcaag | 6200 |
| 6201 | gatggtagaa | atgttgtcgg | tccttgcaca | cgaatattac | gccatttgcc | 6250 |
| 6251 | tgcatattca | aacagctctt | ctacgataag | ggcacaaatc | gcatcgtgga | 6300 |
| 6301 | acgtttgggc | ttctaccgat | ttagcagttt | gatacacttt | ctctaagtat | 6350 |
| 6351 | ccacctgaat | cataaatcgg | caaaatagag | aaaaattgac | catgtgtaag | 6400 |
| 6401 | cggccaatct | gattccacct | gagatgcata | atctagtaga | atctcttcgc | 6450 |
| 6451 | tatcaaaatt | cacttccacc | ttccactcac | cggttgtcca | ttcatggctg | 6500 |
| 6501 | aactctgctt | cctctgttga | catgacacac | atcatctcaa | tatccgaata | 6550 |
| 6551 | gggcccatca | gtctgacgac | caagagagcc | ataaacacca | atagccttaa | 6600 |
| 6601 | catcatcccc | atatttatcc | aatattcgtt | ccttaatttc | atgaacaatc | 6650 |
| 6651 | ttcattcttt | cttctctagt | cattattatt | ggtccattca | ctattctcat | 6700 |
| 6701 | tcccttttca | gataatttta | gatttgcttt | tctaaataag | aatatttgga | 6750 |
| 6751 | gagcaccgtt | cttattcagc | tattaataac | tcgtcttcct | aagcatcctt | 6800 |
| 6801 | caatcctttt | aataacaatt | atagcatcta | atcttcaaca | aactggcccg | 6850 |
| 6851 | tttgttgaac | tactctttaa | taaaataatt | tttccgttcc | caattccaca | 6900 |
| 6901 | ttgcaataat | agaaaatcca | tcttcatcgg | ctttttcgtc | atcatctgta | 6950 |
| 6951 | tgaatcaaat | cgccttcttc | tgtgtcatca | aggtttaatt | ttttatgtat | 7000 |
| 7001 | ttcttttaac | aaaccaccat | aggagattaa | ccttttacgg | tgtaaacctt | 7050 |
| 7051 | cctccaaatc | agacaaacgt | ttcaaattct | tttcttcatc | atcggtcata | 7100 |
| 7101 | aaatccgtat | cctttacagg | atattttgca | gtttcgtcaa | ttgccgattg | 7150 |
| 7151 | tatatccgat | ttatatttat | ttttcggtcg | aatcatttga | acttttacat | 7200 |
| 7201 | ttggatcata | gtctaatttc | attgcctttt | tccaaaattg | aatccattgt | 7250 |
| 7251 | ttttgattca | cgtagttttc | tgtattctta | aaataagttg | gttccacaca | 7300 |
| 7301 | taccaataca | tgcatgtgct | gattataaga | attatcttta | ttatttattg | 7350 |
| 7351 | tcacttccgt | tgcacgcata | aaaccaacaa | gatttttatt | aattttttta | 7400 |
| 7401 | tattgcatca | ttcggcgaaa | tccttgagcc | atatctgaca | aactcttatt | 7450 |
| 7451 | taattcttcg | ccatcataaa | catttttaac | tgttaatgtg | agaaacaacc | 7500 |
| 7501 | aacgaactgt | tggcttttgt | ttaataactt | cagcaacaac | cttttgtgac | 7550 |
| 7551 | tgaatgccat | gtttcattgc | tctcctccag | ttgcacattg | gacaaagcct | 7600 |
| 7601 | ggatttacaa | aaccacactc | gatacaactt | tctttcgcct | gtttcacgat | 7650 |
| 7651 | tttgtttata | ctctaatatt | tcagcacaat | cttttactct | ttcagccttt | 7700 |
| 7701 | ttaaattcaa | gaatatgcag | aagttcaaag | taatcaacat | tagcgatttt | 7750 |
| 7751 | cttttctctc | catggtctca | cttttccact | ttttgtcttg | tccactaaaa | 7800 |
| 7801 | cccttgattt | ttcatctgaa | taaatgctac | tattaggaca | cataatatta | 7850 |
| 7851 | aaagaaaccc | ccatctattt | agttatttgt | ttagtcactt | ataactttaa | 7900 |
| 7901 | cagatggggt | ttttctgtgc | aaccaatttt | aagggttttc | aatactttaa | 7950 |
| 7951 | aacacataca | taccaacact | tcaacgcacc | tttcagcaac | taaaataaaa | 8000 |
| 8001 | atgacgttat | ttctatatgt | atcaagataa | gaaagaacaa | gttcaaaacc | 8050 |
| 8051 | atcaaaaaaa | gacacctttt | caggtgcttt | ttttatttta | taaactcatt | 8100 |
| 8101 | ccctgatctc | gacttcgttc | tttttttacc | tctcggttat | gagttagttc | 8150 |
| 8151 | aaattcgttc | tttttaggtt | ctaaatcgtg | tttttcttgg | aattgtgctg | 8200 |
| 8201 | ttttatcctt | taccttgtct | acaaacccct | taaaaacgtt | tttaaaggct | 8250 |
| 8251 | tttaagccgt | ctgtacgttc | cttaag | 8276 |
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文献和实验相关实验
” (PBD), by which the enzyme binds to its intracellular anchorage sites and to at least a fraction of its substrates. Recent evidence indicates that the inhibition of the PBD by small molecules is feasible and might allow for the discovery of highly
300 30 0-14 200 ZR03 ZirChrom-PBD 反相
-89 个氨基酸的N末端调节区的部分p21-activated kinase (PAK-1)相结合。PAK-1的这个区域,被认为是p21偶联区域即P21binding domain (PBD),特异性的与活性形式的Rac1相作用,最终提供了一个优秀的工具以分离细胞样本中的活性Rac1. 细胞被镁盐缓冲溶液（ magnesium lysis buffer)所裂解; 接着裂解液需要采用GST-agarose beads (not provided)进行预清除.在这一步，少量的细胞裂解液能够储存
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pBD9
询价
