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- 2025年07月12日
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- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
组织甲基化DNA免疫沉淀试剂盒
- 库存：
1
- 供应商：
上海信裕
- 组织甲基化DNA免疫沉淀试剂盒详细信息：
-
Product Overview
The EpiQuik™ Tissue Methylated DNA Immunoprecipitation (MDIP) Kit is a complete set of essential components which uses a proprietary and unique procedure/composition to enrich methylated DNA from various mammalian tissues. In the assay, an antibody specific to methyl cytosine is used to immunoprecipitate methylated genomic DNA. The immunoprecipitated methylated fractions can be then used for a standard DNA detection. The kit is suitable for combining the specificity of methylated DNA immunoprecipitation with qualitative and quantitative PCR, and southern blot as well as DNA microarray.
WHY CHOOSE THE EPIQUIK™ TISSUE MDIP KIT?- Directly immunoprecipitate methylated fractions of DNA from tissue lysates.
- Highly efficient enrichment of methylated DNA: > 95%
- The fastest procedure available, which can be finished within 3 and a half hours.
- Strip microwell format makes the assay flexible: manual or high throughput.
- Columns for DNA purification are included: save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Simple, reliable, and consistent assay conditions.
组织甲基化DNA免疫沉淀试剂盒Principle & Procedure
The EpiQuik™ Tissue Methylated DNA Immunopricipitation (MDIP) Kit contains all reagents required for carrying out a successful methylated DNA immunoprecipitation directly from mammalian tissues. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA in the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
SCHEMATIC PROCEDURE: 
组织甲基化DNA免疫沉淀试剂盒Product Components
CP1 (wash buffer)
CP2 (antibody buffer)
CP3 (lysis buffer)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Homogenizing buffer
Normal mouse IgG (1 mg/ml)*
Anti-5-methylcytosine (1mg/ml)*
Proteinase K (10 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide
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xy-E10671 肾上腺髓质素(ADM)ELISA试剂盒 检测范围
xy-E10672 免疫反应性生长激素(irGH)ELISA试剂盒 检测范围
xy-E10673 硫酸褪黑色素(MS)ELISA试剂盒 检测范围
xy-E10674 长效甲状腺刺激素(LATS)ELISA试剂盒 检测范围
xy-E10675 抗利尿激素/血管加压素/精氨酸加压素(ADH/VP/AVP)ELISA试剂盒 检测范围
xy-E10676 甘丙肽/甘丙素(GAL)ELISA试剂盒 检测范围
xy-E10677 促胰液素/分泌素受体(SR)ELISA试剂盒 检测范围
xy-E10678 雌激素受体(ER)ELISA试剂盒 检测范围
xy-E10679 超敏生长激素(U S-GH)ELISA试剂盒 检测范围
xy-E10680 超敏前列腺特异性抗原(PSA-U S)ELISA试剂盒 检测范围
xy-E10681 苯丙酸诺龙/苯丙酸去甲睾酮(NP)ELISA试剂盒 检测范围
xy-E10682 16α羟基雌酮1(16-α OHE-1)ELISA试剂盒 检测范围
xy-E10683 氢化可的松(HYD)ELISA试剂盒 检测范围
xy-E10684 糖皮质激素受体β(GR-β)ELISA试剂盒 检测范围
xy-E10685 糖皮质激素受体α(GR-α)ELISA试剂盒 检测范围
xy-E10686 高敏三碘甲状腺原氨酸(u-T3)ELISA试剂盒 检测范围
xy-E10687 黄体生成素释放激素(LHRH)ELISA试剂盒 检测范围
xy-E10688 8异前列腺素(8-iso-PG)ELISA试剂盒 检测范围
xy-E10689 肾素(Renin)ELISA试剂盒 检测范围
xy-E10690 肾上腺素(EPI)ELISA试剂盒 检测范围
xy-E10691 雌二醇受体(ER)ELISA试剂盒 检测范围
xy-E10692 甲状旁腺激素相关蛋白(PTHrP)ELISA试剂盒 检测范围
xy-E10693 血管紧张素原(aGT)ELISA试剂盒 检测范围
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xy-E10696 甲状腺素抗体(TAb)ELISA试剂盒 检测范围
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* Spin the solution down to the bottom before use.组织甲基化DNA免疫沉淀试剂盒User Guide & MSDS
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文献和实验多囊卵巢综合征(Polycystic ovary syndrome, PCOS)是一种复杂的、病因不明的疾病，最新研究结果表明，胰岛素耐受(insulin resistance , IR)在PCOS的发生过程中起了重要的作用。本研究主要致力于探究IR在PCOS发生过程中的分子机制，运用基因组DNA甲基化免疫沉淀技术（methylated DNA immunoprecipitation , MeDIP)，分析了正常人与PCOS病人基因组中有差异的甲基化基因，此外，还鉴别了胰岛素不耐
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