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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
1年
- 英文名:
pBact-myc(pBactmyc)
- 库存:
大量
- 供应商:
信裕生物
质粒编号:
质粒名称: pBact-myc
其他名称:
质粒大小(bp): 5620
抗性:
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
| Notes: |
Restriction digests of the clone give the following sizes (kb): HindIII–5.6, PstI–4.8, 0.45, 0.35; EcoRI–5.6. (ATCC staff) Shuttle expression vector allowing production of a fusion protein containing an amino terminal c-myc epitope tag (FQKLISEEDLN), that can be used for immunological detection of the recombinant protein in transfected cells. [1] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Matus A.L. Hosts: E.coli, E.coli HB101, mammalian cells.
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质粒图谱:
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文献和实验Chromatin Immunoprecipitation Assays for Myc and N-Myc
Myc and N-Myc have widespread impacts on the chromatin state within cells, both in a gene-specific and genome-wide manner. Our laboratory uses functional genomic methods including chromatin immunoprecipitation (ChIP), ChIP-chip
Myc is a transcriptional coordinator of a wide range of intracellular and extracellular processes required for cell proliferation. These processes are tightly regulated in physiological conditions but hijacked when Myc is oncogenically
In this chapter, we discuss in detail two essential methods used to evaluate the interaction of Myc with another protein of interest: co-immunoprecipitation (Co-IP) and in vitro pull-down assays. Co-IP is a method that, by immunoaffinity
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