pATH21

名称	编号	规格	价格	手册
pATH21

质粒编号:
质粒名称: pATH21
其他名称:
质粒大小（bp）: 3793
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Created by Moore, July 1995, under contract with NCBI. The nucleotides remaining in the codon after cutting the vector with the given enzyme are (S-not for 5'-juncture because of stop codon in frame): BamHI-2S, EcoRI-2S, HindIII-2, KpnI-3S, PstI-3, SacI-3S, SalI-2, SmaI-3S, SphI-3, XbaI-2S, XmaI-1S. Restriction digests of the clone give the following sizes (kb): BamHI–4.1; EcoRI–4.1; HindIII–4.1. (ATCC staff) Nucleotide 1 is the first in the trp fragment, at the beginning of a PvuII site 261 nucleotides upstream of the transcription start site. The multiple cloning site follows codon 323 of trpE. E.coli containing pATH vectors should be grown on medium supplemented with tryptophan to prevent expression. Storage as DNA at -20C is preferred; long term storage as cells may result in selection of non-expressing promoter mutations. Fusion proteins are produced in E.coli usually in an insoluble form which facilitates purification.Hybrid proteins larger than 90 kDa are not expressed as efficiently as shorter ones. To prevent recircularization of the vector, the depositors recommend including 30 – 50 U of calf alkaline phosphatase in the restriction enzyme digestion used to prepare the vector. This is one of a series of vectors (ATCC 37695-37703) designed to facilitate expression of an open reading frame as a trpE fusion protein. Medium is -1 1065 plus ampicillin (50 ug/ml) & tryptophan (10 ug/ml). Hosts: E.coli RR1, E.coli. Related vectors: pATH1, pUC18, pUC8.
质粒图谱:

质粒序列: /td

1	cagctgtggt	gtcatggtcg	gtgatcgcta	gggtgccgag	cgcatctcga	50
51	ctgcacggtg	caccaatgct	tctggcgtca	ggtagttatt	ggaaagctgt	100
101	ggtatggctg	tgcaggtcgt	aaatcactgc	ataactcgct	gctgcctaag	150
151	gcgcactccc	gttctggata	atgttttttg	cgccgacatc	ataacggttc	200
201	tggcaaatat	tctgaaatga	gctgttgaca	attaatcatc	gaactagtta	250
251	actagtacgc	aagttcacgt	aaaaagggta	tcgacaatga	aagcaatttt	300
301	cgtactgaaa	ggttggtggc	gcacttcctg	aaacgggcag	tgtattcacc	350
351	atgcgtaaag	caatcagata	cccagcccgc	ctaatgagcg	ggcttttttt	400
401	tgaacaaaat	tagagaataa	caatgcaaac	acaaaaaccg	actctcgaac	450
451	tgctaacctg	cgaaggcgct	tatcgcgaca	atcccaccgc	gctttttcac	500
501	cagttgtgtg	gggatcgtcc	ggcaacgctg	ctgctggaat	ccgcagatat	550
551	cgacagcaaa	gatgatttaa	aaagcctgct	gctggtagac	agtgcgctgc	600
601	gcattacagc	tttaggtgac	actgtcacaa	tccaggcact	ttccggcaac	650
651	ggcgaagccc	tcctggcact	actggataac	gccctgcctg	cgggtgtgga	700
701	aagtgaacaa	tcaccaaact	gccgtgtgct	gcgcttcccc	cctgtcagtc	750
751	cactgctgga	tgaagacgcc	cgcttatgct	ccctttcggt	ttttgacgct	800
801	ttccgtttat	tgcagaatct	gttgaatgta	ccgaaggaag	aacgagaagc	850
851	catgttcttc	agcggcctgt	tctcttatga	ccttgtggcg	ggatttgaag	900
901	atttaccgca	actgtcagcg	gaaaataact	gccctgattt	ctgtttttat	950
951	ctcgctgaaa	cgctgatggt	gattgaccat	cagaaaaaaa	gcacccgtat	1000
1001	tcaggccagc	ctgtttgctc	cgaatgaaga	agaaaaacaa	cgtctcactg	1050
1051	ctcgcctgaa	cgaactacgt	cagcaactga	ccgaagccgc	gccgccgctg	1100
1101	ccagtggttt	ccgtgccgca	tatgcgttgt	gaatgtaatc	agagcgatga	1150
1151	agagttcggt	ggcgtagtgc	gtttgttgca	aaaagcgatt	cgcgctggag	1200
1201	aaattttcca	ggtggtgcca	tctcgccgtt	tctctctgcc	ctgcccgtca	1250
1251	ccgctggcgg	cctattacgt	gctgaaaaag	agtaatccca	gcccgtacat	1300
1301	gttttttatg	caggataatg	atttcaccct	atttggcgcg	tcgccggaaa	1350
1351	gctcgctcaa	gtatgatgcc	accagccgcc	agattgagat	ccccgggcga	1400
1401	gctcgaattc	gagctcgccc	agcttgcatg	cctgcaggtc	gactctagag	1450
1451	gatccccggg	taccgagctc	gcgatgataa	gctgtcaaac	atgagaatta	1500
1501	attcttgaag	acgaaagggc	ctcgtgatac	gcctattttt	ataggttaat	1550
1551	gtcatgataa	taatggtttc	ttagacgtca	ggtggcactt	ttcggggaaa	1600
1601	tgtgcgcgga	acccctattt	gtttattttt	ctaaatacat	tcaaatatgt	1650
1651	atccgctcat	gagacaataa	ccctgataaa	tgcttcaata	atattgaaaa	1700
1701	aggaagagta	tgagtattca	acatttccgt	gtcgccctta	ttcccttttt	1750
1751	tgcggcattt	tgccttcctg	tttttgctca	cccagaaacg	ctggtgaaag	1800
1801	taaaagatgc	tgaagatcag	ttgggtgcac	gagtgggtta	catcgaactg	1850
1851	gatctcaaca	gcggtaagat	ccttgagagt	tttcgccccg	aagaacgttt	1900
1901	tccaatgatg	agcactttta	aagttctgct	atgtggcgcg	gtattatccc	1950
1951	gtgttgacgc	cgggcaagag	caactcggtc	gccgcataca	ctattctcag	2000
2001	aatgacttgg	ttgagtactc	accagtcaca	gaaaagcatc	ttacggatgg	2050
2051	catgacagta	agagaattat	gcagtgctgc	cataaccatg	agtgataaca	2100
2101	ctgcggccaa	cttacttctg	acaacgatcg	gaggaccgaa	ggagctaacc	2150
2151	gcttttttgc	acaacatggg	ggatcatgta	actcgccttg	atcgttggga	2200
2201	accggagctg	aatgaagcca	taccaaacga	cgagcgtgac	accacgatgc	2250
2251	ctgtagcaat	ggcaacaacg	ttgcgcaaac	tattaactgg	cgaactactt	2300
2301	actctagctt	cccggcaaca	attaatagac	tggatggagg	cggataaagt	2350
2351	tgcaggacca	cttctgcgct	cggcccttcc	ggctggctgg	tttattgctg	2400
2401	ataaatctgg	agccggtgag	cgtgggtctc	gcggtatcat	tgcagcactg	2450
2451	gggccagatg	gtaagccctc	ccgtatcgta	gttatctaca	cgacggggag	2500
2501	tcaggcaact	atggatgaac	gaaatagaca	gatcgctgag	ataggtgcct	2550
2551	cactgattaa	gcattggtaa	ctgtcagacc	aagtttactc	atatatactt	2600
2601	tagattgatt	taaaacttca	tttttaattt	aaaaggatct	aggtgaagat	2650
2651	cctttttgat	aatctcatga	ccaaaatccc	ttaacgtgag	ttttcgttcc	2700
2701	actgagcgtc	agaccccgta	gaaaagatca	aaggatcttc	ttgagatcct	2750
2751	ttttttctgc	gcgtaatctg	ctgcttgcaa	acaaaaaaac	caccgctacc	2800
2801	agcggtggtt	tgtttgccgg	atcaagagct	accaactctt	tttccgaagg	2850
2851	taactggctt	cagcagagcg	cagataccaa	atactgtcct	tctagtgtag	2900
2901	ccgtagttag	gccaccactt	caagaactct	gtagcaccgc	ctacatacct	2950
2951	cgctctgcta	atcctgttac	cagtggctgc	tgccagtggc	gataagtcgt	3000
3001	gtcttaccgg	gttggactca	agacgatagt	taccggataa	ggcgcagcgg	3050
3051	tcgggctgaa	cggggggttc	gtgcacacag	cccagcttgg	agcgaacgac	3100
3101	ctacaccgaa	ctgagatacc	tacagcgtga	gcattgagaa	agcgccacgc	3150
3151	ttcccgaagg	gagaaaggcg	gacaggtatc	cggtaagcgg	cagggtcgga	3200
3201	acaggagagc	gcacgaggga	gcttccaggg	ggaaacgcct	ggtatcttta	3250
3251	tagtcctgtc	gggtttcgcc	acctctgact	tgagcgtcga	tttttgtgat	3300
3301	gctcgtcagg	ggggcggagc	ctatggaaaa	acgccagcaa	cgcggccttt	3350
3351	ttacggttcc	tggccttttg	ctggcctttt	gctcacatgt	tctttcctgc	3400
3401	gttatcccct	gattctgtgg	ataaccgtat	taccgccttt	gagtgagctg	3450
3451	ataccgctcg	ccgcagccga	acgaccgagc	gcagcgagtc	agtgagcgag	3500
3501	gaagcggaag	agcgcctgat	gcggtatttt	ctccttacgc	atctgtgcgg	3550
3551	tatttcacac	cgcatatggt	gcactctcag	tacaatctgc	tctgatgccg	3600
3601	catagttaag	ccagtataca	ctccgctatc	gctacgtgac	tgggtcatgg	3650
3651	ctgcgccccg	acacccgcca	acacccgctg	acgcgccctg	acgggcttgt	3700
3701	ctgctcccgg	catccgctta	cagacaagct	gtgaccgtct	ccgggagctg	3750
3751	catgtgtcag	aggttttcac	cgtcatcacc	gaaacgcgcg	agg	3793