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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 服务名称:
抗体及蛋白荧光标记、酶标记服务
- 提供商:
北京博奥森生物技术有限公司
一、服务内容
1、可接收的待标记样品包含抗体IgG、大分子蛋白、小分子多肽、小分子化合物等。
2、可提供标记服务的标记物有:
A.酶标记物:碱性磷酸酶(AP/ALP),辣根过氧化物酶(HRP);
B.生物素(Biotin);
C.常规荧光素:FITC,Rhodamine B(RBITC);
D.Bioss自有知识产权荧光素:AbBy Fluor®系列:AbBy Fluor® 350,405,488,555,594,647,680,750等;
E.荧光素Alexa Fluor® 系列:Alexa Fluor® 350,405,488,555,594,647,680,750等;
F.Cy系列荧光素:Cy3,Cy5,Cy5.5,Cy7;
G.荧光蛋白:藻红蛋白(PE),别藻蓝蛋白(APC);
H.荧光串联标记:PE-Cy荧光素系列:PE-Cy5,PE-Cy5.5,PE-Cy7;
APC-Cy荧光素系列:APC-Cy5.5,APC-Cy7;
PerCP-Cy荧光素系列:PerCP-Cy5.5;
I.胶体金标记Co-Au。
北京博奥森标记抗体发表文献:
文献名称:The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression.
期刊名称:Nature. 2016 Apr 14;532(7598):245-9. doi: 10.1038/nature17403. Epub 2016 Apr 6.
影响因子:(IF)41.456
产品应用:bs-5805R-FITC | RIPK-1 Polyclonal Antibody, FITC Conjugated | IHC-F
产品应用:bs-7596R-A647 | RIP3 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated | IHC-F
研究机构:S. Arthur Localio Laboratory, Department of Surgery, New York University School of Medicine; Department of Cell Biology, New York University School of Medicine; Department of Pathology, New York University School of Medicine; Cold Spring Harbor Laboratories
文献名称:Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a.
期刊名称:Cell Stem Cell. 2012 Jun 14;10(6):759-70. doi: 10.1016/j.stem.2012.02.022.
影响因子:(IF)25.315
产品应用:bs-0008R-PE | Rabbit Anti-Nestin Polyclonal Antibody | FCM
产品应用:bs-2358R-PE | Rabbit Anti-FOXA2/HNF 3beta Polyclonal Antibody, PE Conjugated | FCM
研究机构:Department of Stem Cell Differentiation, Institute for Frontier Medical Sciences, Kyoto University; Laboratory of Reproductive Biology, Graduate School of Agriculture; Experimental Research Center for Infectious Disease, Institute for Virus Research, Kyoto University; Cancer Institute, Japanese Foundation for Cancer Research, Tokyo.
文献名称:Transcriptional profiling of mouse B cell terminal differentiation defines a signature for antibody-secreting plasma cells.
期刊名称:Nat Immunol. 2015 Jun;16(6):663-73. doi: 10.1038/ni.3154. Epub 2015 Apr 20.
影响因子:(IF)24.973
产品应用:bs-9425R-PE | Rabbit Anti-TSPAN15 Polyclonal Antibody, PE Conjugated | FCM
研究机构:The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Department of Computing and Information Systems, The University of Melbourne; Department of Medical Biology, The University of Melbourne; Department of Pathology, The University of Melbourne; Department of Microbiology & Immunology, The University of Melbourne;
文献名称:Pten loss in the bone marrow leads to G-CSF-mediated HSC mobilization.
期刊名称:J Exp Med. 2013 Oct 21;210(11):2337-49. doi: 10.1084/jem.20122768. Epub 2013 Oct 14.
影响引子:(IF)13.912
产品应用:bs-2478R-PE | RABBIT ANTI-CCL12/MCP5 POLYCLONAL ANTIBODY, PE CONJUGATED | FCM
研究机构:Deutsches Krebsforschungszentrum (DKFZ); Heidelberg Institute for Stem Cell Technology and Experimental Medicine; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Science, Ecole Polytechnique Fédérale de Lausanne (EPFL); Department of Molecular and Medical Pharmacology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research; Department of Hematology, Universitätsklinikum Essen, Westdeutsches Tumorzentrum (WTZ); Department of Laboratory Medicine and Comprehensive Cancer Center, University of Californi


常见荧光素参数(Fluorescence Parameters):

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文献和实验酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否,因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体,它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体,其次要有良好的制备方法。目前,高质量的酶(如辣根过氧化物酶,简称HRP)国内已有商品供应。高质量的抗体则可通过提取纯化而获得。在制备方法上,宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。
问:我想知道,用什么方法进行酶标记可以非常有效呐?过碘酸钠标记怎么样?答:第一节 间接法测抗体基本原理将特异性抗原包被在固相载体上,形成固相抗原,加入待检标本(含相应抗体),其中抗体与固相抗原形成抗原-抗体复合物,再加入酶标记的抗抗体(抗人免疫球蛋白抗体,亦称二抗),与上述抗原-抗体复合物结合。此时加入底物,复合物上的酶则催化底物而显色。试剂与设备纯化抗原。酶标抗体(辣根过氧化物酶标记抗人免疫球蛋白)底物 (邻苯二胺OPD或四甲联苯胺TM 底物缓冲液(Na2HPO4 0.184g +构椽酸
2.特异性强 即抗IgG与IgG在免疫电泳上只有一条沉淀线,与IgG的全血清也只有一条沉淀线,这才算是特异性的单价抗体。 3.效价高 一般琼脂扩散鉴定为1︰64以上才算合格。
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