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- Signalway Antibody
- 详询
- ek1858
- USA
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
详询
- 供应商：
莱富赛生物
- 检测范围：
详询
- 检测方法：
详询
- 应用：
ELISA
- 适应物种：
Rat
- 标记物：
详询
- 样本：
详询
- 规格：
1x96T
Detect Range:3.12-200 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Sample Volume:100ul
Assay Time: 3-5h
Detection wavelength: 450 nm
产品详细信息请点击：Rat Hemoglobin subunit alpha-1/2 ELISA Kit
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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