- ￥10600
- GeneTex
- 美国
- GTX60952-02
- 2025年07月14日
- ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic GLP-1(7-17)
- 亚型：
IgG2a, Kappa
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4℃.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX60952-02
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ELISA
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
GLP1
- 抗体英文名：
GLP1 antibody [10] (Biotin)
- 抗体名：
GLP1 抗体 [10] (Biotin)
- 规格：
50 μl
The calibration curve of a sandwich assay for Non-amidated GLP-1 using GTX60954 as the capture antibody and GTX60952 -02 as the biotinylated detection antibody.
This uses GTX60951 as capture antibody in combination with biotinylated detection antibody GTX60952 -02 exclusively to measure GLP-1(7-36)amide in biological samples. GLP-1(7-36)amide is the principal active form of the peptide, and has been estimated to make up about 70% of the physiological response. Relative to measuring both active forms of GLP-1 together, the responses are reduced by not measuring the approximately 30% contribution of GLP-1(7-37) to the overall response. There is no cross-reactivity with any extended or truncated physiological form of GLP-1(7-36)amide. Specimens must be collected into tubes containing an adequate amount of DPP-4 inhibitor (in addition to routine peptidase inhibitors) to prevent the rapid in vitro degradation of the active peptide.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
Biotin labeling and hybridization of probe to chromosomes
Biotin labeling and hybridization of probe to chromosomes Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Biotin labeling of plasmid or genomic DNA. Remove slides
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