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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -2480℃ for several months.
- 英文名:
BRL54443
- 库存:
详询
- 供应商:
详询
- CAS号:
57477-39-1
- 规格:
2mg/10mg/50mg/1ml/10mM
| 规格: | 2mg | 产品价格: | ¥198.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥582.0 |
| 规格: | 50mg | 产品价格: | ¥2288.0 |
| 规格: | 1ml/10mM | 产品价格: | ¥815.0 |
ID:T2459
PURITY:99%+
Formula:C14H18N2O
MolWt:230.31
Appearance:灰色粉末
Solubility:G4200 mM in DMSO
Bioactivity:BRL 54443 is a 5-HT1E and 5-HT1F receptor agonist with pKi of 8.7 and 9.25, respectively, with a weak binding affinity for 5-HT1A, 5-HT1B, 5-HT1D receptors.
Status:
Pathways:Neuroscience
Target:5-HT Receptor agonist
Receptor:5-HT1F;5-HT1E;5-HT1A;5-HT1D;5-HT1B
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文献和实验Protocol for competitive RT-PCR
that containes a T7 RNA promoter sequence. The in vitro transcription of the cloned fragments is performed using T7 RNA polymerase (e.g. Gibco BRL). The internal standard RNA is then treated with RNase-free DNase I (e.g. Gibco BRL) to remove the plasmid DNA
DNA Preparation from Adherent Cells
Section of Cancer Genomics,Genetics Branch,NCI National Institutes of Health Reagents Chloroform Mallinckrodt,cat.4440 EDTA,0.5 M Ethanol,100% Ethanol,70% Isoamyl Alcohol Sigma,cat.I-3643 Phenol Gibco BRL,Cat.15513-039 Phosphate Buffered
You will need for 1° cDNA synthesis: 5 x SuperScript RTase buffer (Gibco-BRL) 5mM methyl dNTPs (Pharmacia, substitute 5m dCTP for dCTP) 100mM DTT (Gibco-BRL) oligo dT primer/adaptor [a-32P] dATP (~400Ci/mmol, Amersham or DuPont) Ribonuclease inhibitor
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