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- 2025年09月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4° – 8°C, do not freeze
- 英文名：
Mouse T Cell Nucleofector™ medium 100 ml
- 供应商：
默瑞生物
- 规格：
PC
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文献和实验Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
cells/ml in culture medium in a suitable tissue culture plate or tissue culture flask. 2) Add Dynabeads Mouse T-Activator CD3/CD28 at a bead-to-cell ratio of 1:1. 3) Add 30 U/ml rIL-2. 4) Incubate in a humidified
The Cell Environment (including types of culture medium)
Iscoves DMEM (Prod. No. 13390) Further enriched modification of DMEM which supports high density growth Leibovitz L-15(Prod. No. L5520, liquid) Designed for CO2 free environments TC 100 (Prod. No. T3160
Fusion of Mouse, Rat, or Hamster Cell
-well plate containing 5 ml/well sterile PBS. Transfer the spleen to a Petri dish containing 50 ml sterile PBS. Prepare a single cell suspension by teasing the tissue with sterile forceps
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