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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Newborn Human Foreskin Fibroblasts (NuFF) feeder cells for pluripotent stem cell culturing, Donor 12, Mitomycin-C treated
- 供应商:
默瑞生物
- 规格:
4-5 million cells/vial
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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs
Culturing Mouse Embryonic Fibroblasts
to human fibroblasts (Parrinello S., et al. Nature Cell Biol, 2003 ). A. Isolating Primary MEFs: B. Passaging primary MEFS: PROCEDURE Setup mouse breeder pairs in the afternoon. Ideally one male
Culturing Mouse Embryonic Fibroblasts
to human fibroblasts (Parrinello S., et al. Nature Cell Biol, 2003 ). A. Isolating Primary MEFs: B. Passaging primary MEFS: PROCEDURE Setup mouse breeder pairs in the afternoon. Ideally one male
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