- 询价
- Signalway Antibody
- 12497
- 2025年09月22日
- WB
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human DYRK1A/B (Phospho-Tyr321/273)
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：Q13627
- 抗体英文名：
DYRK1A/B (Phospho-Tyr321/273) Antibody
- 规格：
50ul/100ul
计算分子量:70kd
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Western blotting: 1:1000
图片:
All lanes : DYRK1A/B (Phospho-Tyr321/273) Antibody at 1/500 dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100nM Calyculin A for 30min whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : HepG2 treated with UV for 15min whole cell lysates
Lane 5 : Mouse brain lysates
Lane 6 : Rat brain lysates
Lysates/proteins at 20 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 70-80 kDa
Exposure time: 5 seconds
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文献和实验的关系理不出头绪。GSK-3 beta 总蛋白减少，p-GSK-3 beta (ser 9)增加，是不是足以说明有活性的GSK-3 beta 减少更多，这样理解不知道对不对，希望给以指点。 galaxy22 楼主你好，请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的，active B catenin是减少的，那我有必要做ser9 和tyr216的磷酸化形式吗？我想研究wnt和akt。。。 棉花糖的温柔
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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