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- 详细信息
- 技术资料
- 免疫原:
The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser1176
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB;IF
- 靶点:
Uniprot:P29474
- 抗体英文名:
eNOS(Phospho-Ser1177) Antibody
- 规格:
50ul/100ul
计算分子量:130-140kD
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:
WB 1:500 - 1:2000. IF 1:200 - 1:1000.
图片:

Western Blot analysis of HuvEc cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000
,

Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using eNOS (Phospho-Ser1176) Antibody. The lane on the right is blocked with the phospho peptide.
,

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
,

Immunofluorescence analysis of Hela cell. 1,NOS3 (phospho Ser1177) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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