NPHS1 Antibody

别名:nephrosis 1, congenital, Finnish type (nephrin), CNF, NPHN, nephrin
配方:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
应用详情:WB 1:500-1:1000;
IHC 1:50-1:100;
ICC/IF 1:50-1:200;

图片:

Western blot analysis of Nephrin expression in HeLa (A), HEK293T (B), PC12 (C) whole cell lysates.
(Predicted band size:130; 134 kDa; Observed band size: 130 kDa)

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Immunohistochemical analysis of Nephrin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Immunofluorescent analysis of Nephrin staining in mouse kidney. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).