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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide corresponding to a region derived from 60-74 amino acids of Human v-Ha-ras Harvey rat sarcoma viral oncogene homolog
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA WB
- 靶点:
Uniprot:P01112
- 抗体英文名:
HRAS Antibody
- 规格:
50ul/100ul
配方:Supplied at 0.7mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
应用详情:
Predicted MW: 21kd
ELISA: 1:1000-1:5000
Western blotting: 1:200-1:1000
图片:

Gel: 10%SDS-PAGE
Lane1: Hela cell lysate
Lane2: HepG2 cell lysate
Lane3: 231 cell lysate
Lysates: 30ug per lane
Primary antibody: 1/400 dilution
Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Exposure time: 20 seconds
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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